LOCUS       V029337                 6324 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029337
VERSION     V029337
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2471
                     /label="Akt2(NM_007434)"
                     /note="Akt2(NM_007434)"
                     /gene="Akt2"
     CDS             2490..2519
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2538..2561
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2581..2597)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2608..3230
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3379..3967)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4296..4343
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4575..5369)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5404..5761)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5763..5867)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5894..6322
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaatgaggta tctgtcatca aagaaggctg gctccacaaa cgtggtgaat
     1081 acatcaagac ctggaggcca cggtacttcc ttctgaagag tgatggatct ttcattgggt
     1141 ataaggagag gcccgaggcc cctgaccaga ccttaccccc cctgaacaat ttctctgtag
     1201 cagaatgcca gctgatgaag actgagaggc cacgacccaa cacctttgtc atacgctgcc
     1261 tgcagtggac cacagtcatc gagaggacct tccatgtaga ctctccagat gagagggaag
     1321 agtggatgcg ggctatccag atggtcgcca acagtctgaa gcagcggggc ccaggtgagg
     1381 acgccatgga ttacaagtgt ggctccccca gtgactcttc cacatctgag atgatggagg
     1441 tagctgtcaa caaggcacgg gccaaagtga ccatgaatga cttcgattat ctcaaactcc
     1501 tcggcaaggg caccttcggc aaggtcattc tggttcgaga gaaggccact ggccgctatt
     1561 atgccatgaa gatcctgcgc aaggaggtca tcattgcaaa ggatgaagtc gcccacacag
     1621 tcacagagag ccgggttctg cagaatacca ggcacccctt ccttacagcc ctcaagtatg
     1681 ccttccagac ccatgaccgc ctatgctttg tgatggagta tgccaacggg ggtgagctgt
     1741 ttttccacct ctctcgggag cgagtcttca cggaggatcg ggcgcgcttt tatggagcag
     1801 agattgtgtc agctctggag tatttgcact cgagagatgt ggtgtaccgt gacatcaagc
     1861 tggaaaacct tatgttggac aaagatggcc acatcaagat cactgacttt ggcttgtgca
     1921 aagagggcat cagtgatgga gccaccatga aaaccttctg tggtaccccg gagtacttgg
     1981 cgcctgaggt gctagaggac aatgactatg ggcgagcagt ggactggtgg gggctgggtg
     2041 tggtcatgta tgagatgatg tgtggccgcc tgccattcta caaccaggac cacgagcgcc
     2101 tctttgagct cattcttatg gaggagatcc gcttcccgcg cacactcggg ccagaggcca
     2161 agtccctgct ggctggactg ctgaagaagg acccaaagca gaggctcggc ggaggtccca
     2221 gtgatgcgaa ggaggtcatg gagcatagat tcttcctcag catcaactgg caggacgtgg
     2281 tacagaaaaa gctcctgcca cccttcaaac ctcaggtcac ttcagaagtg gacacaaggt
     2341 actttgatga cgagttcacc gcccagtcca tcacaatcac acccccagac cgatatgaca
     2401 gcctggaccc gctggaactg gaccagcgga cgcacttccc ccagttctcc tactcagcca
     2461 gcatccgaga gacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
     2521 cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaaacg gccggccgcg
     2581 gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc cccagtgcct
     2641 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2701 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2761 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2821 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2881 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2941 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3001 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3061 accactgctc ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc
     3121 cagacacagc ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt
     3181 ggcactgtcc tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg
     3241 ccagcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4081 ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg ctatggcagg
     4141 gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg gggggtgggg
     4201 tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg gggtatcgac
     4261 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa caccgtgcgt
     4321 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     4381 tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat ccccgcgctg
     4441 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     4501 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     4561 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     4621 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     4681 tcagcaatat cacgggtagc caacgctatg tcctgatagc gatccgccac acccagccgg
     4741 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     4801 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     4861 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     4921 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     4981 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     5041 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     5101 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     5161 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     5221 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     5281 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     5341 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatcg
     5401 atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     5461 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     5521 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     5581 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5641 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5701 gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     5761 ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5881 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5941 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6001 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6061 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6121 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6181 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6241 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6301 taacaaaaat ttaacgcgaa tttt
//
