LOCUS V020505 9128 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020505 VERSION V020505 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..3808 /label="ATP2A2(NM_170665)" /note="ATP2A2(NM_170665)" /gene="ATP2A2" CDS 3815..3880 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 3904..4492 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4558..4606 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4808..5236 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5250..5579 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5646..6440 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6616..6737 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6786..6802) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6810..6826 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6834..6864) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6879..6900 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7188..7776) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7947..8807) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8808..8912) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatggagaa cgcgcacacc aagacggtgg aggaggtgct 721 gggccacttc ggcgtcaacg agagtacggg gctgagcctg gaacaggtca agaagcttaa 781 ggagagatgg ggctccaacg agttaccggc tgaagaagga aaaaccttgc tggaacttgt 841 gattgagcag tttgaagact tgctagttag gattttatta ctggcagcat gtatatcttt 901 tgttttggct tggtttgaag aaggtgaaga aacaattaca gcctttgtag aaccttttgt 961 aattttactc atattagtag ccaatgcaat tgtgggtgta tggcaggaaa gaaatgctga 1021 aaatgccatc gaagccctta aggaatatga gcctgaaatg ggcaaagtgt atcgacagga 1081 cagaaagagt gtgcagcgga ttaaagctaa agacatagtt cctggtgata ttgtagaaat 1141 tgctgttggt gacaaagttc ctgctgatat aaggttaact tccatcaaat ctaccacact 1201 aagagttgac cagtcaattc tcacaggtga atctgtctct gtcatcaagc acactgatcc 1261 cgtccctgac ccacgagctg tcaaccaaga taaaaagaac atgctgtttt ctggtacaaa 1321 cattgctgct gggaaagcta tgggagtggt ggtagcaact ggagttaaca ccgaaattgg 1381 caagatccgg gatgaaatgg tggcaacaga acaggagaga acaccccttc agcaaaaact 1441 agatgaattt ggggaacagc tttccaaagt catctccctt atttgcattg cagtctggat 1501 cataaatatt gggcacttca atgacccggt tcatggaggg tcctggatca gaggtgctat 1561 ttactacttt aaaattgcag tggccctggc tgtagcagcc attcctgaag gtctgcctgc 1621 agtcatcacc acctgcctgg ctcttggaac tcgcagaatg gcaaagaaaa atgccattgt 1681 tcgaagcctc ccgtctgtgg aaacccttgg ttgtacttct gttatctgct cagacaagac 1741 tggtacactt acaacaaacc agatgtcagt ctgcaggatg ttcattctgg acagagtgga 1801 aggtgatact tgttccctta atgagtttac cataactgga tcaacttatg cacctattgg 1861 agaagtgcat aaagatgata aaccagtgaa ttgtcaccag tatgatggtc tggtagaatt 1921 agcaacaatt tgtgctcttt gtaatgactc tgctttggat tacaatgagg caaagggtgt 1981 gtatgaaaaa gttggagaag ctacagagac tgctctcact tgcctagtag agaagatgaa 2041 tgtatttgat accgaattga agggtctttc taaaatagaa cgtgcaaatg cctgcaactc 2101 agtcattaaa cagctgatga aaaaggaatt cactctagag ttttcacgtg acagaaagtc 2161 aatgtcggtt tactgtacac caaataaacc aagcaggaca tcaatgagca agatgtttgt 2221 gaagggtgct cctgaaggtg tcattgacag gtgcacccac attcgagttg gaagtactaa 2281 ggttcctatg acctctggag tcaaacagaa gatcatgtct gtcattcgag agtggggtag 2341 tggcagcgac acactgcgat gcctggccct ggccactcat gacaacccac tgagaagaga 2401 agaaatgcac cttgaggact ctgccaactt tattaaatat gagaccaatc tgaccttcgt 2461 tggctgcgtg ggcatgctgg atcctccgag aatcgaggtg gcctcctccg tgaagctgtg 2521 ccggcaagca ggcatccggg tcatcatgat cactggggac aacaagggca ctgctgtggc 2581 catctgtcgc cgcatcggca tcttcgggca ggatgaggac gtgacgtcaa aagctttcac 2641 aggccgggag tttgatgaac tcaacccctc cgcccagcga gacgcctgcc tgaacgcccg 2701 ctgttttgct cgagttgaac cctcccacaa gtctaaaatc gtagaatttc ttcagtcttt 2761 tgatgagatt acagctatga ctggcgatgg cgtgaacgat gctcctgctc tgaagaaagc 2821 cgagattggc attgctatgg gctctggcac tgcggtggct aaaaccgcct ctgagatggt 2881 cctggcggat gacaacttct ccaccattgt ggctgccgtt gaggaggggc gggcaatcta 2941 caacaacatg aaacagttca tccgctacct catctcgtcc aacgtcgggg aagttgtctg 3001 tattttcctg acagcagccc ttggatttcc cgaggctttg attcctgttc agctgctctg 3061 ggtcaatctg gtgacagatg gcctgcctgc cactgcactg gggttcaacc ctcctgatct 3121 ggacatcatg aataaacctc cccggaaccc aaaggaacca ttgatcagcg ggtggctctt 3181 tttccgttac ttggctattg gctgttacgt cggcgctgct accgtgggtg ctgctgcatg 3241 gtggttcatt gctgctgacg gtggtccaag agtgtccttc taccagctga gtcatttcct 3301 acagtgtaaa gaggacaacc cggactttga aggcgtggat tgtgcaatct ttgaatcccc 3361 atacccgatg acaatggcgc tctctgttct agtaactata gaaatgtgta acgccctcaa 3421 cagcttgtcc gaaaaccagt ccttgctgag gatgcccccc tgggagaaca tctggctcgt 3481 gggctccatc tgcctgtcca tgtcactcca cttcctgatc ctctatgtcg aacccttgcc 3541 actcatcttc cagatcacac cgctgaacgt gacccagtgg ctgatggtgc tgaaaatctc 3601 cttgcccgtg attctcatgg atgagacgct caagtttgtg gcccgcaact acctggaacc 3661 tggtaaagag tgtgtgcagc ctgccaccaa atcctgctcg ttctcggcat gcaccgatgg 3721 gatttcctgg ccgtttgtgc tgctcataat gcccctggtg atctgggtct atagcacaga 3781 cactaacttt agcgatatgt tctggtctct cgaggactac aaagaccatg acggtgatta 3841 taaagatcat gacatcgact acaaggatga cgatgacaag tagtgagggc ccgatatctc 3901 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 3961 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 4021 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 4081 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 4141 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 4201 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 4261 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 4321 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 4381 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 4441 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaaac 4501 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg 4561 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt 4621 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 4681 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 4741 gccaacgtcg gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg 4801 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc 4861 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt 4921 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc 4981 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt 5041 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt 5101 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt 5161 gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa 5221 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 5281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 5341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 5401 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 5461 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 5521 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 5581 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt 5641 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 5701 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 5761 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 5821 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 5881 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 5941 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 6001 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 6061 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 6121 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 6181 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 6241 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 6301 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 6361 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 6421 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg 6481 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 6541 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 6601 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6721 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 6781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 6841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 6901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 7021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 7081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 7141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 7201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 7261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 7321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 7381 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 7441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 7501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 7561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 7621 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 7681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 7741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 7801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 7861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 8941 gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg 9001 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 9061 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 9121 tgcttagg //