LOCUS       V031493                 6350 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031493
VERSION     V031493
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             998..2512
                     /label="ATF2(NM_001880)"
                     /note="ATF2(NM_001880)"
                     /gene="ATF2"
     CDS             2516..2545
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2564..2587
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2607..2623)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2634..3256
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3405..3993)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4322..4369
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4601..5395)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5430..5787)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5789..5893)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5920..6348
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccatg aaattcaagt tacatgtgaa
     1021 ttctgccagg caatacaagg acctgtggaa tatgagtgat gacaaaccct ttctatgtac
     1081 tgcgcctgga tgtggccagc gttttaccaa cgaggatcat ttggctgtcc ataaacataa
     1141 acatgagatg acactgaaat ttggtccagc acgtaatgac agtgtcattg tggctgatca
     1201 gaccccaaca ccaacaagat tcttgaaaaa ctgtgaagaa gtgggtttgt ttaatgagtt
     1261 ggcgagtcca tttgagaatg aattcaagaa agcttcagaa gatgacatta aaaaaatgcc
     1321 tctagattta tcccctcttg caacacctat cataagaagc aaaattgagg agccttctgt
     1381 tgtagaaaca actcaccagg atagtccttt acctcaccca gagtctacta ccagtgatga
     1441 gaaggaagta ccattggcac aaactgcaca gcccacatca gctattgttc gtccagcatc
     1501 attacaggtt cccaatgtgc tgcttacaag ttctgactca agtgtaatta ttcagcaggc
     1561 agtaccttca ccaacctcaa gtactgtaat cacccaggca ccatcctcta acaggccaat
     1621 tgtccctgta ccaggcccat ttcctcttct gttacatctt cctaatggac aaaccatgcc
     1681 tgttgctatt cctgcatcaa ttacaagttc taatgtgcat gttccagctg cagtcccact
     1741 cgttcgacca gtcaccatgg tgcctagtgt tccaggaatc ccaggtcctt cctctcccca
     1801 accagtacag tcagaagcaa aaatgagatt aaaagctgct ttgacccagc aacatcctcc
     1861 agttaccaat ggtgatactg tcaaaggtca tggtagcgga ttggttagga ctcagtcaga
     1921 ggaatctcga ccgcagtcat tacaacagcc agccacatcc actacagaaa ctccggcttc
     1981 tccagctcac acaactccac agacccaaag tacaagtggt cgtcggagaa gagcagctaa
     2041 cgaagatcct gatgaaaaaa ggagaaagtt tttagagcga aatagagcag cagcttcaag
     2101 atgccgacaa aaaaggaaag tctgggttca gtctttagag aagaaagctg aagacttgag
     2161 ttcattaaat ggtcagctgc agagtgaagt caccctgctg agaaatgaag tggcacagct
     2221 gaaacagctt cttctggctc ataaagattg ccctgtaacc gccatgcaga agaaatctgg
     2281 ctatcatact gctgataaag atgatagttc agaagacatt tcagtgccga gtagtccaca
     2341 tacagaagct atacagcata gttcggtcag cacatccaat ggagtcagtt caacctccaa
     2401 ggcagaagct gtagccactt cagtcctcac ccagatggcg gaccagagta cagagcctgc
     2461 tctttcacag atcgttatgg ctccttcctc ccagtcacag ccctcaggaa gtctcgagca
     2521 gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca aggatgacga
     2581 cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat cccgggtggc
     2641 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     2701 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     2761 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     2821 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     2881 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     2941 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     3001 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     3061 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa
     3121 aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac
     3181 cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt
     3241 agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca ctcaaaggcg
     3301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4141 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
     4201 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
     4261 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     4321 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     4381 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
     4441 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     4501 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     4561 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     4621 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     4681 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     4741 gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     4801 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     4861 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     4921 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     4981 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     5041 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     5101 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     5161 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     5221 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     5281 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     5341 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     5401 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct
     5461 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     5521 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     5581 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     5641 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     5701 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     5761 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
     5821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     5941 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6001 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6061 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6121 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6181 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6241 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6301 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
