LOCUS       V038613                14604 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038613
VERSION     V038613
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             756..9284
                     /label="APC(NM_000038)"
                     /note="APC(NM_000038)"
                     /gene="APC"
     CDS             9291..9356
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    9380..9968
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    10034..10082
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      10284..10712
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10726..11055
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             11122..11916
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    12092..12213
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(12262..12278)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12286..12302
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12310..12340)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12355..12376
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12664..13252)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13423..14283)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14284..14388)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgag ctcgaattcg ccaccatggc tgcagcttca tatgatcagt
      781 tgttaaagca agttgaggca ctgaagatgg agaactcaaa tcttcgacaa gagctagaag
      841 ataattccaa tcatcttaca aaactggaaa ctgaggcatc taatatgaag gaagtactta
      901 aacaactaca aggaagtatt gaagatgaag ctatggcttc ttctggacag attgatttat
      961 tagagcgtct taaagagctt aacttagata gcagtaattt ccctggagta aaactgcggt
     1021 caaaaatgtc cctccgttct tatggaagcc gggaaggatc tgtatcaagc cgttctggag
     1081 agtgcagtcc tgttcctatg ggttcatttc caagaagagg gtttgtaaat ggaagcagag
     1141 aaagtactgg atatttagaa gaacttgaga aagagaggtc attgcttctt gctgatcttg
     1201 acaaagaaga aaaggaaaaa gactggtatt acgctcaact tcagaatctc actaaaagaa
     1261 tagatagtct tcctttaact gaaaattttt ccttacaaac agatatgacc agaaggcaat
     1321 tggaatatga agcaaggcaa atcagagttg cgatggaaga acaactaggt acctgccagg
     1381 atatggaaaa acgagcacag cgaagaatag ccagaattca gcaaatcgaa aaggacatac
     1441 ttcgtatacg acagctttta cagtcccaag caacagaagc agagaggtca tctcagaaca
     1501 agcatgaaac cggctcacat gatgctgagc ggcagaatga aggtcaagga gtgggagaaa
     1561 tcaacatggc aacttctggt aatggtcagg gttcaactac acgaatggac catgaaacag
     1621 ccagtgtttt gagttctagt agcacacact ctgcacctcg aaggctgaca agtcatctgg
     1681 gaaccaaggt ggaaatggtg tattcattgt tgtcaatgct tggtactcat gataaggatg
     1741 atatgtcgcg aactttgcta gctatgtcta gctcccaaga cagctgtata tccatgcgac
     1801 agtctggatg tcttcctctc ctcatccagc ttttacatgg caatgacaaa gactctgtat
     1861 tgttgggaaa ttcccggggc agtaaagagg ctcgggccag ggccagtgca gcactccaca
     1921 acatcattca ctcacagcct gatgacaaga gaggcaggcg tgaaatccga gtccttcatc
     1981 ttttggaaca gatacgcgct tactgtgaaa cctgttggga gtggcaggaa gctcatgaac
     2041 caggcatgga ccaggacaaa aatccaatgc cagctcctgt tgaacatcag atctgtcctg
     2101 ctgtgtgtgt tctaatgaaa ctttcatttg atgaagagca tagacatgca atgaatgaac
     2161 tagggggact acaggccatt gcagaattat tgcaagtgga ctgtgaaatg tatgggctta
     2221 ctaatgacca ctacagtatt acactaagac gatatgctgg aatggctttg acaaacttga
     2281 cttttggaga tgtagccaac aaggctacgc tatgctctat gaaaggctgc atgagagcac
     2341 ttgtggccca actaaaatct gaaagtgaag acttacagca ggttattgcg agtgttttga
     2401 ggaatttgtc ttggcgagca gatgtaaata gtaaaaagac gttgcgagaa gttggaagtg
     2461 tgaaagcatt gatggaatgt gctttagaag ttaaaaagga atcaaccctc aaaagcgtat
     2521 tgagtgcctt atggaatttg tcagcacatt gcactgagaa taaagctgat atatgtgctg
     2581 tagatggtgc acttgcattt ttggttggca ctcttactta ccggagccag acaaacactt
     2641 tagccattat tgaaagtgga ggtgggatat tacggaatgt gtccagcttg atagctacaa
     2701 atgaggacca caggcaaatc ctaagagaga acaactgtct acaaacttta ttacaacact
     2761 taaaatctca tagtttgaca atagtcagta atgcatgtgg aactttgtgg aatctctcag
     2821 caagaaatcc taaagaccag gaagcattat gggacatggg ggcagttagc atgctcaaga
     2881 acctcattca ttcaaagcac aaaatgattg ctatgggaag tgctgcagct ttaaggaatc
     2941 tcatggcaaa taggcctgcg aagtacaagg atgccaatat tatgtctcct ggctcaagct
     3001 tgccatctct tcatgttagg aaacaaaaag ccctagaagc agaattagat gctcagcact
     3061 tatcagaaac ttttgacaat atagacaatt taagtcccaa ggcatctcat cgtagtaagc
     3121 agagacacaa gcaaagtctc tatggtgatt atgtttttga caccaatcga catgatgata
     3181 ataggtcaga caattttaat actggcaaca tgactgtcct ttcaccatat ttgaatacta
     3241 cagtgttacc cagctcctct tcatcaagag gaagcttaga tagttctcgt tctgaaaaag
     3301 atagaagttt ggagagagaa cgcggaattg gtctaggcaa ctaccatcca gcaacagaaa
     3361 atccaggaac ttcttcaaag cgaggtttgc agatctccac cactgcagcc cagattgcca
     3421 aagtcatgga agaagtgtca gccattcata cctctcagga agacagaagt tctgggtcta
     3481 ccactgaatt acattgtgtg acagatgaga gaaatgcact tagaagaagc tctgctgccc
     3541 atacacattc aaacacttac aatttcacta agtcggaaaa ttcaaatagg acatgttcta
     3601 tgccttatgc caaattagaa tacaagagat cttcaaatga tagtttaaat agtgtcagta
     3661 gtagtgatgg ttatggtaaa agaggtcaaa tgaaaccctc gattgaatcc tattctgaag
     3721 atgatgaaag taagttttgc agttatggtc aatacccagc cgacctagcc cataaaatac
     3781 atagtgcaaa tcatatggat gataatgatg gagaactaga tacaccaata aattatagtc
     3841 ttaaatattc agatgagcag ttgaactctg gaaggcaaag tccttcacag aatgaaagat
     3901 gggcaagacc caaacacata atagaagatg aaataaaaca aagtgagcaa agacaatcaa
     3961 ggaatcaaag tacaacttat cctgtttata ctgagagcac tgatgataaa cacctcaagt
     4021 tccaaccaca ttttggacag caggaatgtg tttctccata caggtcacgg ggagccaatg
     4081 gttcagaaac aaatcgagtg ggttctaatc atggaattaa tcaaaatgta agccagtctt
     4141 tgtgtcaaga agatgactat gaagatgata agcctaccaa ttatagtgaa cgttactctg
     4201 aagaagaaca gcatgaagaa gaagagagac caacaaatta tagcataaaa tataatgaag
     4261 agaaacgtca tgtggatcag cctattgatt atagtttaaa atatgccaca gatattcctt
     4321 catcacagaa acagtcattt tcattctcaa agagttcatc tggacaaagc agtaaaaccg
     4381 aacatatgtc ttcaagcagt gagaatacgt ccacaccttc atctaatgcc aagaggcaga
     4441 atcagctcca tccaagttct gcacagagta gaagtggtca gcctcaaaag gctgccactt
     4501 gcaaagtttc ttctattaac caagaaacaa tacagactta ttgtgtagaa gatactccaa
     4561 tatgtttttc aagatgtagt tcattatcat ctttgtcatc agctgaagat gaaataggat
     4621 gtaatcagac gacacaggaa gcagattctg ctaataccct gcaaatagca gaaataaaag
     4681 aaaagattgg aactaggtca gctgaagatc ctgtgagcga agttccagca gtgtcacagc
     4741 accctagaac caaatccagc agactgcagg gttctagttt atcttcagaa tcagccaggc
     4801 acaaagctgt tgaattttct tcaggagcga aatctccctc caaaagtggt gctcagacac
     4861 ccaaaagtcc acctgaacac tatgttcagg agaccccact catgtttagc agatgtactt
     4921 ctgtcagttc acttgatagt tttgagagtc gttcgattgc cagctccgtt cagagtgaac
     4981 catgcagtgg aatggtaagt ggcattataa gccccagtga tcttccagat agccctggac
     5041 aaaccatgcc accaagcaga agtaaaacac ctccaccacc tcctcaaaca gctcaaacca
     5101 agcgagaagt acctaaaaat aaagcaccta ctgctgaaaa gagagagagt ggacctaagc
     5161 aagctgcagt aaatgctgca gttcagaggg tccaggttct tccagatgct gatactttat
     5221 tacattttgc cacggaaagt actccagatg gattttcttg ttcatccagc ctgagtgctc
     5281 tgagcctcga tgagccattt atacagaaag atgtggaatt aagaataatg cctccagttc
     5341 aggaaaatga caatgggaat gaaacagaat cagagcagcc taaagaatca aatgaaaacc
     5401 aagagaaaga ggcagaaaaa actattgatt ctgaaaagga cctattagat gattcagatg
     5461 atgatgatat tgaaatacta gaagaatgta ttatttctgc catgccaaca aagtcatcac
     5521 gtaaagcaaa aaagccagcc cagactgctt caaaattacc tccacctgtg gcaaggaaac
     5581 caagtcagct gcctgtgtac aaacttctac catcacaaaa caggttgcaa ccccaaaagc
     5641 atgttagttt tacaccgggg gatgatatgc cacgggtgta ttgtgttgaa gggacaccta
     5701 taaacttttc cacagctaca tctctaagtg atctaacaat cgaatcccct ccaaatgagt
     5761 tagctgctgg agaaggagtt agaggagggg cacagtcagg tgaatttgaa aaacgagata
     5821 ccattcctac agaaggcaga agtacagatg aggctcaagg aggaaaaacc tcatctgtaa
     5881 ccatacctga attggatgac aataaagcag aggaaggtga tattcttgca gaatgcatta
     5941 attctgctat gcccaaaggg aaaagtcaca agcctttccg tgtgaaaaag ataatggacc
     6001 aggtccagca agcatctgcg tcttcttctg cacccaacaa aaatcagtta gatggtaaga
     6061 aaaagaaacc aacttcacca gtaaaaccta taccacaaaa tactgaatat aggacacgtg
     6121 taagaaaaaa tgcagactca aaaaataatt taaatgctga gagagttttc tcagacaaca
     6181 aagattcaaa gaaacagaat ttgaaaaata attccaaggt cttcaatgat aagctcccaa
     6241 ataatgaaga tagagtcaga ggaagttttg cttttgattc acctcatcat tacacgccta
     6301 ttgaaggaac tccttactgt ttttcacgaa atgattcttt gagttctcta gattttgatg
     6361 atgatgatgt tgacctttcc agggaaaagg ctgaattaag aaaggcaaaa gaaaataagg
     6421 aatcagaggc taaagttacc agccacacag aactaacctc caaccaacaa tcagctaata
     6481 agacacaagc tattgcaaag cagccaataa atcgaggtca gcctaaaccc atacttcaga
     6541 aacaatccac ttttccccag tcatccaaag acataccaga cagaggggca gcaactgatg
     6601 aaaagttaca gaattttgct attgaaaata ctccggtttg cttttctcat aattcctctc
     6661 tgagttctct cagtgacatt gaccaagaaa acaacaataa agaaaatgaa cctatcaaag
     6721 agactgagcc ccctgactca cagggagaac caagtaaacc tcaagcatca ggctatgctc
     6781 ctaaatcatt tcatgttgaa gataccccag tttgtttctc aagaaacagt tctctcagtt
     6841 ctcttagtat tgactctgaa gatgacctgt tgcaggaatg tataagctcc gcaatgccaa
     6901 aaaagaaaaa gccttcaaga ctcaagggtg ataatgaaaa acatagtccc agaaatatgg
     6961 gtggcatatt aggtgaagat ctgacacttg atttgaaaga tatacagaga ccagattcag
     7021 aacatggtct atcccctgat tcagaaaatt ttgattggaa agctattcag gaaggtgcaa
     7081 attccatagt aagtagttta catcaagctg ctgctgctgc atgtttatct agacaagctt
     7141 cgtctgattc agattccatc ctttccctga aatcaggaat ctctctggga tcaccatttc
     7201 atcttacacc tgatcaagaa gaaaaaccct ttacaagtaa taaaggccca cgaattctaa
     7261 aaccagggga gaaaagtaca ttggaaacta aaaagataga atctgaaagt aaaggaatca
     7321 aaggaggaaa aaaagtttat aaaagtttga ttactggaaa agttcgatct aattcagaaa
     7381 tttcaggcca aatgaaacag ccccttcaag caaacatgcc ttcaatctct cgaggcagga
     7441 caatgattca tattccagga gttcgaaata gctcctcaag tacaagtcct gtttctaaaa
     7501 aaggcccacc ccttaagact ccagcctcca aaagccctag tgaaggtcaa acagccacca
     7561 cttctcctag aggagccaag ccatctgtga aatcagaatt aagccctgtt gccaggcaga
     7621 catcccaaat aggtgggtca agtaaagcac cttctagatc aggatctaga gattcgaccc
     7681 cttcaagacc tgcccagcaa ccattaagta gacctataca gtctcctggc cgaaactcaa
     7741 tttcccctgg tagaaatgga ataagtcctc ctaacaaatt atctcaactt ccaaggacat
     7801 catcccctag tactgcttca actaagtcct caggttctgg aaaaatgtca tatacatctc
     7861 caggtagaca gatgagccaa cagaacctta ccaaacaaac aggtttatcc aagaatgcca
     7921 gtagtattcc aagaagtgag tctgcctcca aaggactaaa tcagatgaat aatggtaatg
     7981 gagccaataa aaaggtagaa ctttctagaa tgtcttcaac taaatcaagt ggaagtgaat
     8041 ctgatagatc agaaagacct gtattagtac gccagtcaac tttcatcaaa gaagctccaa
     8101 gcccaacctt aagaagaaaa ttggaggaat ctgcttcatt tgaatctctt tctccatcat
     8161 ctagaccagc ttctcccact aggtcccagg cacaaactcc agttttaagt ccttcccttc
     8221 ctgatatgtc tctatccaca cattcgtctg ttcaggctgg tggatggcga aaactcccac
     8281 ctaatctcag tcccactata gagtataatg atggaagacc agcaaagcgc catgatattg
     8341 cacggtctca ttctgaaagt ccttctagac ttccaatcaa taggtcagga acctggaaac
     8401 gtgagcacag caaacattca tcatcccttc ctcgagtaag cacttggaga agaactggaa
     8461 gttcatcttc aattctttct gcttcatcag aatccagtga aaaagcaaaa agtgaggatg
     8521 aaaaacatgt gaactctatt tcaggaacca aacaaagtaa agaaaaccaa gtatccgcaa
     8581 aaggaacatg gagaaaaata aaagaaaatg aattttctcc cacaaatagt acttctcaga
     8641 ccgtttcctc aggtgctaca aatggtgctg aatcaaagac tctaatttat caaatggcac
     8701 ctgctgtttc taaaacagag gatgtttggg tgagaattga ggactgtccc attaacaatc
     8761 ctagatctgg aagatctccc acaggtaata ctcccccggt gattgacagt gtttcagaaa
     8821 aggcaaatcc aaacattaaa gattcaaaag ataatcaggc aaaacaaaat gtgggtaatg
     8881 gcagtgttcc catgcgtacc gtgggtttgg aaaatcgcct gaactccttt attcaggtgg
     8941 atgcccctga ccaaaaagga actgagataa aaccaggaca aaataatcct gtccctgtat
     9001 cagagactaa tgaaagttct atagtggaac gtaccccatt cagttctagc agctcaagca
     9061 aacacagttc acctagtggg actgttgctg ccagagtgac tccttttaat tacaacccaa
     9121 gccctaggaa aagcagcgca gatagcactt cagctcggcc atctcagatc ccaactccag
     9181 tgaataacaa cacaaagaag cgagattcca aaactgacag cacagaatcc agtggaaccc
     9241 aaagtcctaa gcgccattct gggtcttacc ttgtgacatc tgttggatcc gactacaaag
     9301 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt
     9361 gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9421 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9481 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9541 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9601 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9661 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9721 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     9781 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9841 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9901 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9961 ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat accggaagga
    10021 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
    10081 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
    10141 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
    10201 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag atctgcgcag
    10261 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    10321 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    10381 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    10441 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    10501 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    10561 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    10621 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa
    10681 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
    10741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    10801 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10861 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10921 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10981 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    11041 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
    11101 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
    11161 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    11221 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    11281 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
    11341 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    11401 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    11461 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    11521 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    11581 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    11641 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
    11701 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    11761 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    11821 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    11881 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat
    11941 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
    12001 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    12061 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
    12121 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    12181 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
    12241 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    12301 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    12361 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    12421 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    12481 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    12541 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    12601 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    12661 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    12721 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    12781 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    12841 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    12901 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    12961 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13021 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    13081 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    13141 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    13201 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    13261 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    13321 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    13381 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    13441 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    13501 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    13561 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    13621 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    13681 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    13741 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    13801 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    13861 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    13921 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    13981 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    14041 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    14101 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    14161 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    14221 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    14281 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    14341 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    14401 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtcgactctc
    14461 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    14521 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
    14581 aattgcatga agaatctgct tagg
//
