LOCUS       V014011                 5912 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014011
VERSION     V014011
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             83..100
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        214..232
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             672..842
                     /gene="mcjA"
                     /label="Microcin J25"
                     /note="Microcin J25 from Escherichia coli. Accession#:
                     Q9X2V7"
     CDS             858..902
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      954..1001
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1038..1493
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1611..2468)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2469..2561)
                     /label="AmpR promoter"
     rep_origin      2642..3230
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3416..3558)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3663..3851)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(4389..4410)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4426..5505)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5506..5583)
                     /label="lacI promoter"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
      121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
      181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
      241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
      301 tgaccatgta cgcaacgctt gaagaagcca ttgacgctgc acgcgaagaa tttcttgcag
      361 acaaccccgg catcgacgcc gaagatgcga atgtgcaaca gttcaatgcc caaaaatacg
      421 ttttgcagga cggcgacatc atgtggcaag ttgagttttt tgccgacgaa ggggaagaag
      481 gtgaatgttt acctatgctt agcggtgaag ccgcgcaaag tgtttttgat ggcgactatg
      541 atgagataga gatacgccag gagtggcagg aagagaatac attacatgaa tgggacgagg
      601 gggaatttca gcttgagcca ccgctggata ccgaggaagg acgcgcagca gctgatgagt
      661 gggatgaacg tattaagcat tttcatttta ataaactgtc ttctggtaaa aaaaataatg
      721 ttccatctcc tgcaaagggg gttatacaaa taaaaaaatc agcatcgcaa ctcacaaaag
      781 gtggtgcagg acatgtgcct gagtattttg tggggattgg tacacctata tctttctatg
      841 gctgactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact
      901 cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa taactagcat
      961 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     1021 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1081 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1141 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1201 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1261 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1321 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1381 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1441 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttctggcgg
     1501 cacgatggca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1921 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2221 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2461 atactcatac tcttcctttt tcaatcatga ttgaagcatt tatcagggtt attgtctcat
     2521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggtcatg accaaaatcc
     2581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2761 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     3421 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct
     3481 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     3541 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3601 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3661 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     3721 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     3781 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     3841 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     3901 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     3961 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     4021 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     4081 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     4141 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     4201 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     4261 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgct agtcatgccc
     4321 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     4381 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     4441 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4501 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     4561 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     4621 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     4681 atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact cggtaatggc
     4741 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     4801 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     4861 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     4921 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     4981 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     5041 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     5101 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     5161 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     5221 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     5281 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     5341 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     5401 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     5461 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     5521 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     5581 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     5641 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     5701 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     5761 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     5821 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatcgatc
     5881 tcgatcccgc gaaattaata cgactcacta ta
//
