LOCUS       Exported                3303 bp DNA     circular SYN 16-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   11111111
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(457..3303,1..456)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        108..126
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             188..220
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      382..429
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        560..664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             665..1525
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1696..2284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2470..2609
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2711..2902)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
       61 cccgtggcca ggacccaacg ctgcccgaga tctcgatccc gcgaaattaa tacgactcac
      121 tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga
      181 tatacatatg gctagcatga ctggtggaca gcaaatgggt cgggattcaa gcttggtacc
      241 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
      301 ctggcggccg ctcgagcaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      361 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      421 ggttttttgc tgaaaggagg aactatatcc ggataattct tgaagacgaa agggcctcgt
      481 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      901 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1201 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1801 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2341 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2401 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2461 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     2521 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     2581 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     2641 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2701 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     2761 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     2821 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     2881 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     2941 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     3001 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     3061 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     3121 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     3181 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     3241 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     3301 aac
//
