LOCUS 40924_17129 8865 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pEGFP-2.attB, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8865) AUTHORS Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K. TITLE A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila JOURNAL Development 140 (11), 2434-2442 (2013) PUBMED 23637332 REFERENCE 2 (bases 1 to 8865) AUTHORS Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K. TITLE Direct Submission JOURNAL Submitted (13-APR-2013) Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland REFERENCE 3 (bases 1 to 8865) TITLE Direct Submission REFERENCE 4 (bases 1 to 8865) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Development"; date: "2013"; volume: "140"; issue: "11"; pages: "2434-2442" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (13-APR-2013) Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8865 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..4136 /label=white dominant eye marker /note="white dominant eye marker" CDS join(479..550,952..1225,1300..1954,2016..2331,2552..2683, 2754..3368) /codon_start=1 /gene="white" /product="Drosophila white gene eye color pigment" /label=mini-white /note="This ia a modified version of the white gene lacking part of the first intron." /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE" protein_bind 4143..4176 /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." misc_feature 4189..4258 /label=multiple cloning site /note="multiple cloning site" CDS 4264..4980 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" intron 5073..5138 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 5268..5288 /codon_start=1 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" polyA_signal 5560..5694 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" protein_bind 5723..5792 /label=attB /note="attB site for the phi-C31 integrase (Groth et al., 2000)" promoter complement(6011..6029) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6050..6066) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(6074..6090) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6098..6128) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(6143..6164) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6452..7040) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7214..8071) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8072..8176) /label=AmpR promoter rep_origin complement(8202..8657) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 8799..8815 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 8822..8840 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" gene 8865 /label=mini-white /note="This modified version of the white gene lacks part of the first intron." ORIGIN 1 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt 61 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat 121 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac 181 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc 241 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct 301 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta 361 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga 421 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat 481 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca 541 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact 601 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc 661 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata 721 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta 781 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa 841 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt 901 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc 961 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg 1021 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac 1081 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga 1141 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg 1201 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga 1261 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg 1321 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc 1381 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg 1441 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc 1501 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaggccatg gtgcggatgc 1561 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt 1621 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg 1681 gcggagaaag gaagcgtctg gcattcgcct ccgaggcact aaccgatccg ccgcttctga 1741 tctgcgatga gcccacctcc ggactggact catttaccgc ccacagcgtc gtccaggtgc 1801 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg 1861 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg 1921 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt 1981 atctagcatt acattacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac 2041 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag 2101 tcccgtgatc ggatcgccaa gatatgcgac aattttgcta ttagcaaagt agcccgggat 2161 atggagcagt tgttggccac caaaaatttg gagaagccac tggagcagcc ggagaatggg 2221 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg 2281 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt 2341 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta 2401 gttagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata 2461 gttttgcaga aaataaataa atttcattta actcgcgaac atgttgaaga tatgaatatt 2521 aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg gcctcatctt 2581 tttgggccaa caactcacgc aagtgggcgt gatgaatatc aacggagcca tcttcctctt 2641 cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc ttgtttagaa 2701 tacatttgca tattaataat ttactaactt tctaatgaat cgattcgatt taggtgttca 2761 cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc tgtgacacat 2821 actttctggg caaaacgatt gccgaattac cgctttttct cacagtgcca ctggtcttca 2881 cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc ttcaactgcc 2941 tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta atatcctgcg 3001 ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata ccattcctgc 3061 tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt 3121 acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg 3181 agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca 3241 tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca 3301 ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca 3361 aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc attattacca 3421 tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa 3481 aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca caagtagact 3541 ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt 3601 ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc 3661 cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca 3721 cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt 3781 ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta 3841 gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat atttccctca 3901 cagctatatt tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt 3961 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta 4021 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa 4081 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactaggcta 4141 gcataacttc gtataatgta tgctatacga agttatgcta gcggatccaa gcttcgcagg 4201 cctcgcaccg gtgaattcgt taacagatct gcggccgcgg ctcgagggta cctctagata 4261 catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 4321 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 4381 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 4441 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 4501 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 4561 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 4621 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 4681 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 4741 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 4801 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 4861 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 4921 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 4981 tgacctagga ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg 5041 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat 5101 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg 5161 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc 5221 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga 5281 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg 5341 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac 5401 tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata 5461 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 5521 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 5581 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 5641 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccactag 5701 tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg 5761 gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt 5821 ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc 5881 tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc 5941 ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca 6001 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt 6061 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 6121 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 6181 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6241 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 6901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 7021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 7081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 7141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 7201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 8041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 8101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 8161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat 8221 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 8281 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 8341 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 8401 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 8461 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 8521 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 8581 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 8641 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc 8701 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 8761 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 8821 gtaatacgac tcactatagg gcgaattggg tacgtaccgg gcccc //