LOCUS       V025817                 7155 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025817
VERSION     V025817
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        123..1301
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     CDS             1364..3871
                     /label="DACT1(NM_016651)"
                     /note="DACT1(NM_016651)"
                     /gene="DACT1"
     polyA_signal    3996..4117
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4124..4579)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4606..4710
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4712..5069
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5104..5898
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6130..6177
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6506..7094
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      481 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      541 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      601 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      661 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      721 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
      781 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
      841 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
      901 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
      961 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1021 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1081 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1141 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1201 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1261 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgct
     1321 agcgtttaaa cttaagcttg gtaccgagct cggatccgcc accatgaagc cgagtccggc
     1381 cgggacggcg aaggagctgg agcctccggc gccggcccga ggcgagcagc gcacggcgga
     1441 gcccgagggg cgctggcggg agaagggcga ggcagacacc gagcggcagc gcacccggga
     1501 gcggcaggag gccacgctgg ccgggctggc ggagctggag tacctgcgcc agcgccaaga
     1561 gctgctggtc aggggcgccc tgcgcggcgc cgggggtgcg ggagccgctg cgccccgcgc
     1621 tggggagcta ctgggggagg cggcgcagcg cagtcgcctg gaggagaagt tcttggagga
     1681 gaacatcttg ctgctaagaa agcaattgaa ctgtttgagg cgaagagatg ctggtttgtt
     1741 gaatcagttg caagagcttg acaagcagat aagtgacctg agactggatg tagaaaagac
     1801 atctgaagag cacctggaga cagacagtcg gcctagctca gggttttatg agctgagtga
     1861 tggggcttca ggatcccttt ccaattcctc taactcggtg ttcagtgagt gtttatccag
     1921 ttgtcattcc agcacctgct tttgcagccc cttggaggcg accttgagtc tctcagatgg
     1981 ttgccccaaa tctgcagatc tcataggatt gttggaatat aaagaaggcc actgtgaaga
     2041 ccaggcctca ggggcagttt gccgttccct ctccacacca caatttaatt cccttgatgt
     2101 cattgcagat gtgaatccca agtaccagtg tgatctggtg tctaaaaacg ggaatgatgt
     2161 atatcgctat cccagtccac ttcatgctgt ggctgtgcag agcccaatgt ttctcctttg
     2221 tctgacgggc aaccctctga gggaagagga caggcttgga aaccatgcca gtgacatttg
     2281 cggtggatct gagctagatg ccgtcaaaac agacagttcc ttaccgtccc caagcagtct
     2341 gtggtctgct tcccatcctt catccagcaa gaaaatggat ggctacattc tgagcctggt
     2401 ccagaaaaaa acacaccctg taaggaccaa caaaccaaga accagcgtga acgctgaccc
     2461 cacgaaaggg cttctgagga acgggagcgt ttgtgtcaga gccccgggcg gtgtctcaca
     2521 gggcaacagt gtgaacctta agaattcgaa acaggcgtgt ctgccctctg gcgggatacc
     2581 ttctctgaac aatgggacat tctccccacc gaagcagtgg tcgaaagaat caaaggccga
     2641 acaagccgaa agcaagaggg tgcccctgcc agagggctgc ccctcaggcg ctgcctccga
     2701 ccttcagagt aagcacctgc caaaaacggc caagccagcc tcgcaagaac atgctcggtg
     2761 ttccgccatt gggacagggg agtcccctaa ggaaagcgct cagctctcag gggcctctcc
     2821 aaaagagagt cctagcagag gccctgcccc gccgcaggag aacaaagttg tacagcccct
     2881 gaaaaagatg tcacagaaaa acagcctgca gggcgtcccc ccggccactc ctcccctgct
     2941 gtctacagct ttccccgtgg aagagaggcc tgccttggat ttcaagagcg agggctcttc
     3001 ccaaagcctg gaggaagcgc acctggtcaa ggcccagttt atcccggggc agcagcccag
     3061 tgtcaggctc caccggggcc acaggaacat gggcgtcgtg aagaactcca gcctgaagca
     3121 ccgcggccca gccctccagg ggctggagaa cggcttgccc accgtcaggg agaaaacgcg
     3181 ggccgggagc aagaagtgtc gcttcccaga tgacttggat acaaataaga aactcaagaa
     3241 agcctcctcc aaggggagga agagtggggg cgggcccgag gctggtgttc ccggcaggcc
     3301 cgcgggcggg ggccacaggg cggggagcag ggcgcatggc cacggacggg aggcggtggt
     3361 ggccaaacct aagcacaagc gaactgacta ccggcggtgg aagtcctcgg ccgagatttc
     3421 ctacgaagag gccctgagga gggcccggcg cggtcgccgg gagaatgtgg ggctgtaccc
     3481 cgcgcctgtg cctctgccct acgccagccc ctacgcctac gtggctagcg actccgagta
     3541 ctcggccgag tgcgagtccc tgttccactc caccgtggtg gacaccagtg aggacgagca
     3601 gagcaattac accaccaact gcttcgggga cagcgagtcg agtgtgagcg agggcgagtt
     3661 cgtgggggag agcacaacca ccagcgactc tgaagaaagc gggggcttaa tttggtccca
     3721 gtttgtccag actctgccca ttcaaacggt aacggcccca gaccttcaca accaccccgc
     3781 aaaaaccttt gtcaaaatta aggcctcaca taacctcaag aagaagatcc tccgctttcg
     3841 gtctggctct ttgaaactga tgacgacggt ttgagcggcc gcgactctag atcataatca
     3901 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3961 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4021 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4081 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     4141 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4201 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4261 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4321 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     4381 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4441 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4501 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4561 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4621 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4681 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     4741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4981 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5041 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     5101 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     5161 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     5221 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     5281 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     5341 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     5401 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     5461 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5521 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5581 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     5641 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5701 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5761 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5821 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5881 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5941 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6001 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6061 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     6121 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     6181 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     6241 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     6301 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     6361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7141 cttttgctca catgt
//
