LOCUS       40924_14875        4938 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDNR-Dual, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Kalnine N, Farmer A, Lin Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella 
            ave., Mountain View, CA 94043, USA
REFERENCE   2  (bases 1 to 4938)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2006) Informatics, Clontech, 1290 Terra Bella ave., Mountain
            View, CA 94043, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..42
                     /label=loxP recombination site
                     /note="loxP recombination site"
                     /regulatory_class="other"
     protein_bind    9..42
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    45..129
                     /note="MCS; multiple cloning site"
     misc_feature    132..136
                     /label=splice donor (SD) site
                     /note="splice donor (SD) site"
     CDS             141..176
                     /label=6xHN
                     /note="6x(His-Asn) affinity tag"
     terminator      complement(212..306)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(330..986)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     protein_bind    complement(1015..1048)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1063..1508
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             1509..2927
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        2998..3102
                     /label=AmpR promoter
     CDS             3103..3960
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4134..4722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4839..4855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4863..4881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgcat aacttcgtat agcatacatt atacgaagtt atcagtcgac ggtaccggac
       61 atatgcccgg gaattcctgc aggatccgct cgagaagctt tctagaccat tcgtttggcg
      121 cgcgggccca ggtgagtggt cataatcata atcataatca taatcataat cacaactagc
      181 ctaggagatc ctggtcatga ctagtgcttg gattctcacc aataaaaaac gcccggcggc
      241 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      301 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
      361 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      421 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      481 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      541 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      601 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      661 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      721 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
      781 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
      841 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
      901 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
      961 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaag atccataact
     1021 tcgtatagca tacattatac gaagttatgc ggccgcgacg tccacatata cctgccgttc
     1081 actattattt agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac
     1141 tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt
     1201 ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga
     1261 ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat
     1321 cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta
     1381 tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc
     1441 catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga
     1501 catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg
     1561 cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata
     1621 aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc
     1681 aaaaaaatga aaaatatcaa gttcctgagt tcgattcgtc cacaattaaa aatatctctt
     1741 ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg
     1801 caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg
     1861 acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa
     1921 acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag
     1981 accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat
     2041 tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta
     2101 acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct
     2161 ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca
     2221 gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat
     2281 acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat
     2341 ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc
     2401 tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc
     2461 taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa
     2521 cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg
     2581 actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg
     2641 gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt
     2701 taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc
     2761 aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag
     2821 acaaacaatc aacgtttgcg cctagcttcc tgctgaacat caaaggcaag aaaacatctg
     2881 ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa aaacgcaaaa
     2941 gaaaatgccg atatcctatt ggcattgacg tcaggtggca cttttcgggg aaatgtgcgc
     3001 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3061 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3121 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3181 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3241 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3301 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3361 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3421 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3481 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3541 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3601 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3661 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3721 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3781 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3841 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3901 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3961 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4021 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4081 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4141 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4201 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4261 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4321 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4381 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4441 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4501 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4561 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4621 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4681 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4741 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4801 cctgattctg tggataaccg tattaccgcc ttacgcgtgt aaaacgacgg ccagtagatc
     4861 tgtaatacga ctcactatag ggcgctagct gctcgccgca gccgaacgac cgagcgcagc
     4921 gagtcagtga gcgaggaa
//
