LOCUS       V014504                10259 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014504
VERSION     V014504
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        191..209
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        294..312
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        654..837
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     CDS             858..2198
                     /gene="tubA"
                     /label="Tubulin beta chain"
                     /note="Tubulin beta chain from Botryotinia fuckeliana.
                     Accession#: P53373"
     terminator      2577..2829
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(3175..3193)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(3262..3293)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3308..3329)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    3697..3721
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5021..5647
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6084..7148
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7217..7411
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7755..7895
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8081..8669)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8759..9550)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9975..9999
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10077..10251)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 aacattaacg tttacaattt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
       61 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
      121 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      181 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
      241 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatccg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgcga cgttaactga
      361 tattgaagga gcactttttg ggcttggctg gagctagtgg aggtcaacaa tgaatgccta
      421 ttttggttta gtcgtccagg cggtgagcac aaaatttgtg tcgtttgaca agatggttca
      481 tttaggcaac tggtcagatc agccccactt gtagcagtag cggcggcgct cgaagtgtga
      541 ctcttattag cagacaggaa ctaggacatt atcatcatct gctgcttggt gcacgataac
      601 ttggtgcgtt tgtcaagcaa ggtaagtgaa cgacccggtc ataccttctt aaggggtttc
      661 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
      721 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
      781 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
      841 gatctggatc gtttcgcatg cgtgagattg tccatcttca aaccggccaa tgtggtaacc
      901 aaattggtgc tgctttctgg caaactatct ctggcgagca cggtcttgac ggttccggtg
      961 tctataatgg tacatccgat ctccaacttg agcgtatgaa cgtctacttc aacgaggctt
     1021 ctggcaacaa gtatgttccc cgtgccgtcc tcgtcgattt ggagccaggt accatggatg
     1081 ctgtccgtgc cggtcctttc ggtcaactct tccgtcccga caacttcgtt ttcggtcaat
     1141 ctggtgctgg taacaactgg gctaagggtc attacactga gggtgctgag cttgtcgacc
     1201 aagttcttga tgttgtccgt cgtgaagctg aaggctgtga ctgccttcaa ggattccaaa
     1261 ttacccactc tctcggtggt ggaactggtg ccggtatggg tacgcttttg atctccaaga
     1321 tccgcgagga gttcccagat cgtatgatgg ctaccttctc cgtcgtccca tcgccaaagg
     1381 tttccgatac cgttgtcgag ccatataacg caactctctc tgtccatcaa ttggttgaga
     1441 actctgacgc gaccttctgt atcgataacg aggctcttta cgatatttgc atgagaacct
     1501 tgaagctcag caacccatct tacggagatc ttaaccactt ggtttccgcc gtcatgtccg
     1561 gtgttaccac ctgtctccgt ttccctggtc aacttaactc agatctccga aagttggctg
     1621 ttaacatggt tccattcccc cgtctccatt tcttcatggt tggatttgct cctttgacca
     1681 gtcgtggcgc acactctttc cgtgctgtca ccgttccaga gttgactcaa caaatgtacg
     1741 accctaagaa catgatggcc gcttccgatt tccgtaacgg tcgttacttg acatgctctg
     1801 ccattttccg tggtaaggtt tccatgaagg aggttgagga ccaaatgcgc aacgtccaaa
     1861 acaagaactc atcctacttc gttgagtgga tccctaacaa cgtccaaacc gccctttgct
     1921 ccattcctcc ccgtggtctc aagatgtcct ccaccttcgt tggtaactcg acatccatcc
     1981 aagaactttt caagcgtgtc ggtgatcaat tcactgctat gttcagaaga aaggctttct
     2041 tgcattggta cactggtgaa ggtatggacg agatggagtt cactgaggct gagtccaaca
     2101 tgaacgattt ggtttccgag tatcaacaat accaggatgc ctcgatctct gagggagagg
     2161 aggagtacga agaggaggtc ccaattgagg gcgaggaata gttcgaaatg accgaccaag
     2221 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     2281 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     2341 tggagttctt cgcccacggg atctctgcgg aacaggcggt cgaaggtgcc gatatcatta
     2401 cgacagcaac ggccgacaag cacaacgcca cgatcctgag cgacaatatg atcgggcccg
     2461 gcgtccacat caacggcgtc ggcggcgact gcccaggcaa gaccgagatg caccgcgata
     2521 tcttgctgcg ttcggatatt ttcgtggagt tcccgccaca gacccggatg atccccgatc
     2581 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     2641 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     2701 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     2761 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     2821 tactagatca cgcgttttcg ggtttacctc ttccagatac agctcaactg caatgcatta
     2881 atgcattgga cctcgcaacc ctagtacgcc cttcaggctc cggcgaagca gaagaatagc
     2941 ttagcagagt ctattttcat tttcgggaga cgagatcaag cagatcaacg gtcgtcaaga
     3001 gacctacgag actgaggaat ccgctcttgg ctccacgcga ctatatattt gtctctaatt
     3061 gtactttgac atgctcctct tctttactct gatagcttga ctatgaaaat tccgtcacca
     3121 gccctgacta gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     3181 gtgagggtta attccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3241 ttatccgctc acaattccac acaacatacg agccggaagh cataaagtgt aaagcctggg
     3301 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3361 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3421 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3481 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3541 ataacgcagg aaagaacatg aaggctggcg taatagcgaa gaggcccgca ccgatcgccc
     3601 ttcccaacag ttgcgcagcc tgaatggcga atgctagagc agcttgagct tggatcagat
     3661 tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     3721 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta
     3781 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     3841 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     3901 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     3961 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     4021 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     4081 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     4141 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     4201 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     4261 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     4321 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     4381 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     4441 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     4501 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     4561 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     4621 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     4681 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     4741 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     4801 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     4861 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     4921 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     4981 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     5041 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     5101 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     5161 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     5221 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     5281 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     5341 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     5401 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     5461 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     5521 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     5581 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     5641 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     5701 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     5761 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     5821 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     5881 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     5941 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     6001 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     6061 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg
     6121 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     6181 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     6241 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     6301 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     6361 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     6421 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     6481 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     6541 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     6601 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     6661 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     6721 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     6781 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     6841 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     6901 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     6961 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     7021 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     7081 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     7141 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     7201 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     7261 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     7321 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     7381 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     7441 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     7501 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     7561 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     7621 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7681 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7741 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     7801 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     7861 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     7921 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7981 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8041 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8101 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8161 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8221 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8281 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8341 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8401 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8461 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8521 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8581 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8641 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8701 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
     8761 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     8821 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
     8881 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     8941 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     9001 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     9061 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     9121 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     9181 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     9241 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     9301 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     9361 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     9421 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     9481 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     9541 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     9601 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
     9661 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     9721 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     9781 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     9841 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     9901 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     9961 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    10021 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc
    10081 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
    10141 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
    10201 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa acttggcaa
//
