LOCUS       pDC316-Opa1(mouse)      6845 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018601
VERSION     V018601
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_signal     190..340
                     /note="packaging signal for adenovirus serotype 5"
                     /label="Ad5 Ψ"
     promoter        470..993
                     /label="mCMV promoter"
     CDS             1006..3939
                     /label="Opa1(NM_001199177)"
                     /note="Opa1(NM_001199177)"
                     /gene="Opa1"
     polyA_signal    3968..4102
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     protein_bind    complement(4122..4155)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
     primer_bind     complement(4177..4193)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4201..4217
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4225..4255)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4270..4291
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4579..5167)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5338..6198)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6199..6303)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gatatactga gtcattaggg
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
      661 taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt actttcccac
      721 cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg tactttccca
      781 tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg aagtgaaagg
      841 gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg gcacgcattc
      901 tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg gtaccgtcgc
      961 agtcttcggt ctgaccaccg tagaacgcag atcgaattcg ccaccatgtg gcgagcaggt
     1021 cgggcggccg tggcctgtga agtctgccaa tccttagtga aacacagttc tggaatacaa
     1081 agaaacgtac cgctccaaaa actccatctg gtttcacgaa gtatttatcg ttcacatcat
     1141 cctgccctca agcttcaaag accccaacta aggacaccat ttcagcagtt ctcttctcta
     1201 actcaccttt cattacataa attgaaactt tctccaatta aatatggcta ccagccccgc
     1261 aggaactttt ggccagcaag gttagctgca agactcttaa aacttcgata tatcatactg
     1321 ggatctgctg ttggaggtgg ctatacagcc aaaaaaacct tcgatgaatg gaaagatatg
     1381 ataccagacc ttagtgacta taagtggatt gtgcctgact ttatatggga aattgatgag
     1441 tatattgatt tggagaaaat tagaaaagcc ctgcccagct cagaagacct tgccagttta
     1501 gctcccgacc tggacaagat tactgagagc ctcagcttgt tgaaggactt cttcactgca
     1561 ggtcccaaat tggttagtga agtcctagaa gtttctgagg cccttctctt gttaggttca
     1621 cctggagaaa cagcatttcg agcaacagat catggatctg aaagtgacaa gcattacagg
     1681 aaggtgtcag acaaagaaaa gattgaccaa cttcaagaag aacttctgca tactcagtta
     1741 aagtatcaga ggatcttgga gcgcctggaa aaggagaaca aagagctgcg gaagctggtg
     1801 ctgcagaagg acgacaaagg catccaccac aggaagctca agaaatcttt gattgatatg
     1861 tattctgaag ttcttgatgt tctttctgat tatgatgcca gttacaatac acaagatcac
     1921 ctaccacggg ttgttgtggt tggagatcag agtgctggga aaaccagtgt gctggaaatg
     1981 attgctcagg cccggatctt cccgagaggg tccggcgaga tgatgacacg ctctccagtg
     2041 aaggtgactc tcagtgaagg ccctcaccat gtggccttgt ttaaagatag ctctcgggaa
     2101 tttgatctca ccaaggagga agatcttgca gcattaagac atgaaatcga actccgaatg
     2161 aggaaaaatg tgaaagaagg ttgtactgtt agccccgaga ccatatctct aaatgtcaaa
     2221 ggccctgggc tgcagaggat ggtgctcgtg gacttgcctg gtgtcatcaa caccgtgaca
     2281 tcaggcatgg ctcccgacac aaaggaaact attttcagta tcagcaaagc ttacatgcag
     2341 aatcctaacg ccatcatcct gtgcatccaa gacggatccg tagatgctga gcgcagtatt
     2401 gttacagact tggtcagtca aatggatcct catggaagaa gaaccatatt tgttttgacc
     2461 aaagtagacc tggcagaaaa aaatgtagcc agtccaagca ggatacaaca gataattgaa
     2521 ggcaagctct tcccaatgaa agctctgggt tattttgctg tcgtaacagg aaaaggaaac
     2581 agctctgaaa gtattgaagc tataagagag tatgaagagg aattttttca gaattcaaaa
     2641 ctgctaaaga caagcatgct aaaggcacac caggtcacca cgagaaatct cagccttgct
     2701 gtgtcagact gcttttggaa aatggttcga gagtcagttg aacaacaggc tgatagtttt
     2761 aaagccacgc gctttaacct agagacggaa tggaagaata actacccgcg cctgcgagag
     2821 ctcgacagga atgaactctt tgaaaaagct aaaaatgaga tcctcgatga ggtcatcagt
     2881 ctgagccagg tcacgccaaa gcactgggag gaaatcctgc agcaatccct gtgggaacga
     2941 gtgtcaacac atgtgattga gaacatctac cttccagctg cccagaccat gaattcggga
     3001 acatttaaca ccacagtaga catcaagctt aaacagtgga ctgacaagca gcttcctaat
     3061 aaagcagtcg aggttgcctg ggagactcta caagaggaat tttcccgctt catgacagaa
     3121 cccaaaggaa aggaacacga cgacatattt gacaaactta aggaggctgt gaaggaggag
     3181 agtatcaagc ggcacaagtg gaacgacttt gccgaggata gcttgagggt tattcagcac
     3241 aatgctttgg aagaccggtc catatcagat aagcaacagt gggatgcagc catttacttc
     3301 atggaagagg cgcttcaagg tcgtctcaag gatactgaaa atgctattga aaacatgatt
     3361 gggccagact ggaaaaagag gtggatgtac tggaagaatc ggacccaaga gcagtgtgtt
     3421 cacaacgaaa ccaagaacga gttggagaag atgctgaagg ttaatgatga gcacccagct
     3481 tacctggcaa gtgatgagat taccacagtc cggaagaacc tggagtctcg aggagtggaa
     3541 gtcgatccaa gcttgattaa ggatacttgg catcaagttt atagaagaca tttcttaaaa
     3601 acagctctaa atcattgtaa cctttgtcgc agaggttttt attactacca gaggcatttt
     3661 atagattctg agctggaatg caatgacgtg gtcctgtttt ggcgaataca gcgcatgctc
     3721 gctatcactg ccaatacatt aaggcagcag cttacaaaca ctgaagttag gcgactagag
     3781 aaaaacgtta aagaggtatt agaagatttt gcagaagacg gtgagaagaa ggttaaattg
     3841 ctcactggca aacgagttca gctggcagaa gatctcaaga aagttagaga aattcaagaa
     3901 aagcttgatg ctttcattga agctcttcac caggagaagt agaagcttga gctcgtcgac
     3961 ttcgagcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4021 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4081 tgtatcttat catgtctgga tcgtctagca tcgaagatcc aataacttcg tatagcatac
     4141 attatacgaa gttataagta gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4201 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4261 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4321 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4381 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4741 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     4801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4981 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     5041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5941 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6301 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     6361 aacctataaa aataggcgta tcactctagg caaaatagca ccctcccgct ccagaacaac
     6421 atacagcgct tcacagcggc agcctaacag tcagccttac cagtaaaaaa gaaaacctat
     6481 taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc acagtgtaaa aaagggccaa
     6541 gtgcagagcg agtatatata ggactaaaaa atgacgtaac ggttaaagtc cacaaaaaac
     6601 acccagaaaa ccgcacgcga acctacgccc agaaacgaaa gccaaaaaac ccacaacttc
     6661 ctcaaatcgt cacttccgtt ttcccacgtt acgtaacttc ccattttaag aaaactacaa
     6721 ttcccaacac atacaagtta ctccgcccta aaacctacgt cacccgcccc gttcccacgc
     6781 cccgcgccac gtcacaaact ccaccccctc attatcatat tggcttcaat ccaaaataag
     6841 gtata
//
