LOCUS       V016545                 8118 bp    DNA     circular SYN 20-JUN-2020
DEFINITION  Exported.
ACCESSION   V016545
VERSION     V016545
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Yuzhen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2019) Department of Public Health Laboratory
            Sciences, West China School of Public HealthSichuan University, No.
            17, Section 3, Renmin South Road, Wuhou District, Chengdu, Sichuan
            610041, China
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2019) Department of Public Health Laboratory Sciences, West
            China School of Public HealthSichuan University, No. 17, Section 3,
            Renmin South Road, Wuhou District, Chengdu, Sichuan 610041, China"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             5798..6529
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga
       61 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      121 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      181 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      241 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
      301 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
      361 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
      421 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
      481 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct
      541 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
      601 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
      661 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
      901 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
      961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     2221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     2521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca
     3001 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt
     3061 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca
     3121 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct
     3181 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt
     3241 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa
     3301 atgaaaaact ccgcagatac acttaaaaaa gttctagaag taaggcaaat cctcctcgga
     3361 gcacgcgttc ctggtcgctt agttctcggc cggatatcca atcttttcca gtaactgctg
     3421 aagaggcttc tagtgcgcca aatcctactc caagtgctaa tccggctggt gggcagacga
     3481 tacttacgcc cacaatcacc acggcggcaa cgatattgac ccagaactct ttctcttctt
     3541 gttcgtcttg gacggattta taatcaaacg cacgagtatg taacatcccc attttaaaat
     3601 cggcttcgct aaccttttgg tccggatttt catgcttcca gacttcgtat tggagggtca
     3661 tttggttggc gtagaagtta tcgccattca ttaagtcctc aatagttgca ttttctttta
     3721 ccgattcaat cacttggggc tgattactgt acgaagacat atacgtttgt gtcgacttag
     3781 ccccaatacg gtttgtagtt gagtatgtcg tgatactcaa gtcttgcatc ccatctacaa
     3841 atgcatccaa cttcttgtaa aaaggctcat caatcagcgt ttcgattttc tcccctgcgt
     3901 gacaactaaa ctgatacagc acctttaagt cttcctcgat ttccccgtac ttgcttcgat
     3961 ggtcggtatg ttggaaatga atcgcgtgat cggtgtctaa gtcacggata tcgtcttccg
     4021 cattctccat atttttcgac actttttcta aatgattaaa taggccacca aaccaattgg
     4081 ctgcattaaa tggagctagg gcacttttgg ctttttacca gtcgtcttct cggtagttta
     4141 cgtctttcat tcatctgtcc tcgactttct ttgttatatg agctgtatat taatgaaagt
     4201 tcatctccaa atcttgttat atgtttcttt taatcaatat aagcaaccat ccttttaaga
     4261 tggttgccca aacctcaaga cactccttgc tagttaattt attggaacta tctaaaaact
     4321 taattaaata gagaaactgc cgcaaaacta agctttgcag cagttaattt tttcaattat
     4381 aacccttact ttgctaattt gtaaagcgct tctgcgtaaa ttgctgtcgc ttttaacaaa
     4441 tcgtcaaaat aactgaattc gtctttttgg tgcatcgtat cttcgcggcc tgggaatagt
     4501 gcgccaaacg ccacacctgt ttccatatga cgtgcataag ttccgccacc aattgcaagt
     4561 aaagtagctt cttctcctgt ttgtttcgta taaacttctt gtaaagtttg aattaatggg
     4621 tggtcttttg gtacgaaaag cggtttggaa tcactgtaat gtgtgtactg tgcattatat
     4681 tcatatacga cagtttgcat tttatttttc agcttatcca tgtttgcagt aactgggtaa
     4741 cggaaattaa gtccgtattt cccaccttcc ccaacatcat aacggataac gccaacattc
     4801 atcgttaatt cgccactttc tttatcttcg tagctaatac caagttttac agcacgagaa
     4861 tcgccaaata agtaatcacg accaaatgta acgaaatcat tagcagcacc agttaattta
     4921 aatttaccta agaaggctac taaatgaaga cctgcattga taccattatt tggttccatt
     4981 gcatgagcag attttccaac catgttaatt ttaactgttt taccgtcttc ttctaacgta
     5041 ccttctactg gatggttagc taagaaagtt ttgaaagtac ttgttagttt atcgtagtct
     5101 ttcacgtttt cgatgatggc actagcatga tctggtaact agtcccgggg cgaattgcat
     5161 ctgaccgaat tttacgtttc cctgaataat tctcatcaat cgtttcatca attttatctt
     5221 tatactttat attttgtgcg ttaatcaaat cataattttt atatgtttcc tcatgattta
     5281 tgtctttatt attatagttt ttattctctc tttgattatg tctttgtatc ccgtttgtat
     5341 tacttgatcc tttaactctg gcaaccctca aaattgaatg agacatgcta cacctccgga
     5401 taataaatat atataaacgt ataaagattt cataaagtct aacacactag acttatttac
     5461 ttcgtaatta agtcgttaaa ccgtgtgctc tacgaccaaa actataaaac ctttaagaac
     5521 tttctttttt tacaagaaaa aagaaattag ataaatctct catatctttt attcaataat
     5581 cgcatccgat tgcagtataa atttaacgat cactcatcat gttcatattt atcagagctc
     5641 gtgctataat tatactaatt ttataaggag gaaaaaatat gggcattttt agtatttttg
     5701 taatcagcac agttcattat caaccaaaca aaaaataagt ggttataatg aatcgttaat
     5761 aagcaaaatt catataacca aattaaagag ggttataatg aacgagaaaa atataaaaca
     5821 cagtcaaaac tttattactt caaaacataa tatagataaa ataatgacaa atataagatt
     5881 aaatgaacat gataatatct ttgaaatcgg ctcaggaaaa ggccatttta cccttgaatt
     5941 agtaaagagg tgtaatttcg taactgccat tgaaatagac cataaattat gcaaaactac
     6001 agaaaataaa cttgttgatc acgataattt ccaagtttta aacaaggata tattgcagtt
     6061 taaatttcct aaaaaccaat cctataaaat atatggtaat ataccttata acataagtac
     6121 ggatataata cgcaaaattg ttcttgatag tatagctaat gagatttatt taatcgtgga
     6181 atacgggttt gctaaaagat tattaaatac aaaacgctca ttggcattac ttttaatggc
     6241 agaagttgat atttctatat taagtatggt tccaagagaa tattttcatc ctaaacctaa
     6301 agtgaatagc tcacttatca gattaagtag aaaaaaatca agaatatcac acaaagataa
     6361 acaaaagtat aattatttcg ttctgaaatg ggttaacaaa gaatacaaga aaatatttac
     6421 aaaaaatcaa tttaacaatt ccttaaaaca tgcaggaatt gacgatttaa acaatattag
     6481 ctttgaacaa ttcttatctc ttgtcaatag ctataaatta tttaataagt aagttaaggg
     6541 atgcataaac tgcatccctt aacttgtttt tcgtgtgcct attttttgtg aatcgtcgac
     6601 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
     6661 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata
     6721 ggcttggtta tgccggtact gccgggcctc ttgcgggatc gatagaaagc gtgagaaaca
     6781 gcgtacagac gatttagaga tgtagaggta cttttatgcc gagaaaactt tttgcgtgtg
     6841 acagtcctta aaatatactt agagcgtaag cgaaagtagt agcgacagct attaactttc
     6901 ggttgcaaag ctctaggatt tttaatggac gcagcgcatc acacgcaaaa aggaaattgg
     6961 aataaatgcg aaatttgaga tgttaattaa agaccttttt gaggtctttt tttcttagat
     7021 ttttggggtt atttagggga gaaaacatag gggggtacta cgacctcccc cctaggtgtc
     7081 cattgtccat tgtccaaaca aataaataaa tattgggttt ttaatgttaa aaggttgttt
     7141 tttatgttaa agtgaaaaaa acagatgttg ggaggtacag tgatagttgt agatagaaaa
     7201 gaagagaaaa aagttgctgt tactttaaga cttacaacag aagaaaatga gatattaaat
     7261 agaatcaaag aaaaatataa tattagcaaa tcagatgcaa ccggtattct aataaaaaaa
     7321 tatgcaaagg aggaatacgg tgcattttaa acaaaaaaag atagacagca ctggcatgct
     7381 gcctatctat gactaaattt tgttaagtgt attagcaccg ttattatatc atgagcgaaa
     7441 atgtaataaa agaaactgaa aacaagaaaa attcaagagg acgtaattgg acatttgttt
     7501 tatatccaga atcagcaaag ccgagtggtt agagtattta aaagagttac acattcaatt
     7561 tgtagtgctc cattacatga tagggatact gatacagaag gtaggatgaa aaaagagcat
     7621 tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa aataataaca
     7681 gaagaattga atgcgactat tccgcagatt gcaggaagtg tgaaaggtct tgtgagatat
     7741 atgcttcaca tggacgatcc taataaattt aaatatcaaa aagaagatat gatagtttat
     7801 ggcggtgtag atgttgatga attattaaag aaaacaacaa cagatagata taaattaatt
     7861 aaagaaatga ttgagtttat tgatgaacaa ggaatcgtag aatttaagag tttaatggat
     7921 tatgcaatga agtttaaatt tgatgattgg ttcccgcttt tatgtgataa ctcggcgtat
     7981 gttattcaag aatatataaa atcaaatcgg tataaatctg accgatagat tttgaattta
     8041 ggtgtcacaa gacactcttt tttcgcacca gcgaaaactg gtttaagccg agtgcgcaaa
     8101 agacataatc gggaattc
//
