LOCUS       V008155                 8420 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008155
VERSION     V008155
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(149..308)
                     /label="CaMV35S polyA terminator"
                     /note="CaMV35S polyA terminator"
                     /regulatory_class="terminator"
     CDS             complement(366..1202)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     transit_peptide complement(1203..1304)
                     /gene="Sul1"
                     /note="pea rbcS ribulose-bisphosphate carboxylase small
                     subunit transit peptide for plastid targeting"
     promoter        complement(1311..1982)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    2142..2166
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3467..4093
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4530..5594
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      5663..5857
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6201..6341
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6527..7115)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7205..7996)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
      121 gcgcgccaag cttgcatgcc tgcaggtcac tggattttgg ttttaggaat tagaaatttt
      181 attgatagaa gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac
      241 atgagcgaaa ccctataaga accctaattc ccttatctgg gaactactca cacattattc
      301 tggagaaaaa tagagagaga tagatttgta gagagagact ggtgattttt gcggactcta
      361 gactaggcat gatctaaccc tcggtctctg gcgtcgcgac tgcgaaattt cgcgagggtt
      421 tccgagatgg tgattgcgct tcgcagatct ccaggcgcgt gggtgcggac gtagtcagcg
      481 ccattgccga tcgcgtgaag ttccgccgca aggctcgctg gacccagatc ctttacagga
      541 aggccaacgg tggcgcccaa gaaggatttc cgcgacaccg agaccaatag cggaagcccc
      601 aacgccgact tcagcttttg aaggttcgac agcacgtgca gcgatgtttc cggtgcgggg
      661 ctcaagaaaa atcccatccc cggatcgagg attagccggt cggcagcgac cccgctccgt
      721 cgcaaggcgg aaacccgcgc ctcgaagaac cgcacaatct cgtcgagcgc gtcttcgggt
      781 cgaaggtgac cggtgcgggt ggcgatgcca tcccgctgcg ctgagtgcat aaccaccagc
      841 ctgcagtccg cctcagcaat atcgggatag agcgcagggt caggaaatcc ttggatatcg
      901 ttcaggtagc ccacgccgcg cttgagcgca tagcgctggg tttccggttg gaagctgtcg
      961 attgaaacac ggtgcatctg atcggacagg gcgtctaaga gcggcgcaat acgtctgatc
     1021 tcatcggccg gcgatacagg cctcgcgtcc ggatggctgg cggccggtcc gacatccacg
     1081 acgtctgatc cgactcgcag catttcgatc gccgcggtga cagcgccggc ggggtctagc
     1141 cgccggctct catcgaagaa ggagtcctcg gtgagattca gaatgccgaa caccgtcacc
     1201 atgcacttta ctcttccacc attgcttgta atggaagtaa tgtcagtgtt gaccttctta
     1261 actgggaatc cagtcatgga tttgaggccg ccgaatgggc ccatctcgag gtcctctcca
     1321 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
     1381 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
     1441 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     1501 cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg taggagccac
     1561 cttccttttc cactatcttc acaataaagt gacagatagc tgggcaatgg aatccgagga
     1621 ggtttccgga tattaccctt tgttgaaaag tctcaattgc cctttggtct tctgagactg
     1681 tatctttgat atttttggag tagacaagtg tgtcgtgctc caccatgtta tcacatcaat
     1741 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     1801 ggggtccatc tttgggacca ctgtcggcag aggcatcttc aacgatggcc tttcctttat
     1861 cgcaatgatg gcatttgtag gagccacctt ccttttccac tatcttcaca ataaagtgac
     1921 agatagctgg gcaatggaat ccgaggaggt ttccggatat taccctttgt tgaaaagtct
     1981 caattgccct ttggtcttct gagactgtat ctttgatatt tttggagtag acaagtgtgt
     2041 cgtgctccac catgttgacc tgcaggcatg caagcttggc tgcaggtcga gatccccggg
     2101 aattctaaga ggagtccacc atggtagatc tgactagtgt ttgacaggat atattggcgg
     2161 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     2221 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     2281 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     2341 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     2401 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     2461 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     2521 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     2581 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     2641 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     2701 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     2761 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     2821 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     2881 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     2941 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     3001 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     3061 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     3121 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     3181 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     3241 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     3301 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     3361 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     3421 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     3481 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     3541 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     3601 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     3661 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     3721 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     3781 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     3841 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     3901 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     3961 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     4021 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     4081 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     4141 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     4201 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     4261 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     4321 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     4381 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     4441 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     4501 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     4561 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     4621 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     4681 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     4741 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     4801 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     4861 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     4921 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     4981 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     5041 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     5101 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     5161 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     5221 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     5281 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     5341 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     5401 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     5461 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     5521 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     5581 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     5641 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     5701 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     5761 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     5821 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     5881 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     5941 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     6001 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     6061 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     6121 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     6181 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     6241 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     6301 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     6361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6961 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     7201 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
     7261 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
     7321 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
     7381 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
     7441 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
     7501 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
     7561 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
     7621 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
     7681 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
     7741 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
     7801 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
     7861 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
     7921 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
     7981 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
     8041 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     8101 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     8161 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     8221 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     8281 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     8341 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     8401 gggagctgtt ggctggctgg
//
