LOCUS       40924_13225        4919 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCR2.1-kanR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     A System for Site-Specific Genetic Manipulation of the Relapsing 
            Fever Spirochete Borrelia hermsii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky 
            Mountain Laboratories, National Institute of Allergy and Infectious 
            Diseases, National Institutes of Health, 903 S. 4th Street, 
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 4919)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4919)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky Mountain 
            Laboratories, National Institute of Allergy and Infectious Diseases,
            National Institutes of Health, 903 S. 4th Street, Hamilton, MT 
            59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(372..1178)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1179..1299)
                     /label=flgB promoter derived from Borrelia hermsii
                     /note="flgB promoter derived from Borrelia hermsii"
                     /regulatory_class="promoter"
     promoter        complement(1376..1394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1401..1417)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1559..1939
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2307..3098
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             3119..3976
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4150..4738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg cttggcgaat
      301 gagctagcgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc
      361 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
      421 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
      481 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
      541 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
      601 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
      661 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
      721 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
      781 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
      841 tcaggatatt cttctaatac ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac
      901 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
      961 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     1021 ttcagaaaca actctggcgc atcgggcttc ccatacaagc gatagattgt cgcacctgat
     1081 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     1141 aatcgcggcc tcgacgtttc ccgttgaata tggctcatat gaacaccctc tatatcacaa
     1201 attgcttttt actatattat ataataattt aaatcttttt gttcgttaat ttttactttt
     1261 tcattaacat agtccaagtt tatttcaatt ttctttaacg ccggcaaggg cgaattctgc
     1321 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat
     1381 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     1441 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     1501 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     1561 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1621 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1681 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttaga
     1741 gctttacggc acctcgaccg caaaaaactt gatttgggtg atggttcacg tagtgggcca
     1801 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1861 ctcttgttcc aaactggaac aacactcaac cctatcgcgg tctattcttt tgatttataa
     1921 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aattcagggc
     1981 gcaagggctg ctaaaggaac cggaacacgt agaaagccag tccgcagaaa cggtgctgac
     2041 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
     2101 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
     2161 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
     2221 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc
     2281 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     2341 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     2401 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     2461 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     2521 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     2581 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcgccttgct cctgccgaga
     2641 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     2701 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     2761 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     2821 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgatccat ggcgatgcct
     2881 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcaacgac tgtggccggc
     2941 tgggtgtggc ggaccgctat caggacatag cgttggatac ccgtgatatt gctgaagagc
     3001 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3061 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattgaaaaa ggaagagtat
     3121 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3181 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3241 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3301 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtcatacac tattatcccg
     3361 tattgacgcc gggcaagagc aactcggtcg ccgggcgcgg tattctcaga atgacttggt
     3421 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3481 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3541 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3601 tcgttgggaa ccggagctga atgaagccat accaaacgac gagagtgaca ccacgatgcc
     3661 tgtagcaatg ccaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3721 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3781 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3841 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3901 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3961 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4021 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4081 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4261 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     4321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     4561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4741 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4801 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4861 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
