LOCUS       V008239                 5008 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008239
VERSION     V008239
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     An efficient vector system for economical and convenient
            epitope-tagging and promoters switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Chang P, Wang W, Chen J.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5008)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5008)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2018) Research Center of Bioenergy "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(195..1052)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1053..1157)
                     /label="AmpR promoter"
     CDS             1272..1298
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1302..1328
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1332..1358
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2871..3989
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     protein_bind    complement(4242..4275)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(join(4441..5008,1..21))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
       61 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
      181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     1021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     1081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaactcgag
     1201 ccgcggaggc ctgggcccca ggtcgactct agaggtaccg ctagctcgcg agcgcgccaa
     1261 ttagatccgt gtacccatac gatgttccag attacgctgg ttacccatac gatgttccag
     1321 attacgctgg ttacccatac gatgttccag attacgctat cgatatccca tggagatcta
     1381 cgctaggatc gatatctcga gacgcgtgat cttggacacc agtaaataag caaatagcta
     1441 aatcatatac gaattaatgt tatgattaag tgtttacgtg agtgcgatat ttttattact
     1501 atcttataca gttgtatata ctctataaaa tgagttgtct attaattaac gatgaatgct
     1561 ttctgggttt acctctccaa cgactctagt ttacttctca atacattcaa ttgtatttga
     1621 tttgtcaata cttcatcatt aatcaattct atagttttgt ttttctcgtt tatttccaaa
     1681 tttaatgcat caattttatt attcaatttg tcgttgattt tggttaatga ttttatggtt
     1741 tgatctctgg cattgattgt ttgtgttagt ttttcattat tgataattaa actatttaag
     1801 ttagttatca actcggtgtt ttcaagtttt aagttttcaa tttctttaga gtttataaga
     1861 tttgtcaaag tttctgaatt gcttgattgg tcctgtagaa gcgtacagtc tgccagactt
     1921 gccacctgac gtctaagaaa ataacttcgt atagcataca ttatacgaag ttatagctcg
     1981 gatccactag taacggccgc cagtgtgctg gaattcgccc ttggatccaa tcatcactgg
     2041 tgggggtcaa gaaagaggtt tgagatcagg tactttggca ccaccattag tagctgggtt
     2101 tggtgaagct gcaagattaa tgaaacaaga atctgcattt gacaagaaac atattgagag
     2161 gttatctact aaattgaaga acggtttgtt atccattcca tctactcagt ttaacggttg
     2221 taataaccca acatatcaat acccaggttg tgtgaatgtt tcctttgcat acattgaagg
     2281 ggaatctttg ttaatggcat tgaaagatat tgcattgagt tctggttctg catgtacctc
     2341 tgcatctttg gaaccatcgt atgttttaca tgctttgggt gccgatgatg ccttggctca
     2401 ttcttccatt agattcggta ttggtagatt caccactgaa gcagaagttg actatgttat
     2461 tcaagcaatt aatgaaagag ttgatttctt gagaaagatg tctccattat gggaaatggt
     2521 tcaagaaggt attgacttga actctatcga atggagtgga cattaaggtt ttttgttttt
     2581 ttgtaatttg tgttgttatg tgtaattatc gtgtatagag taatttcgga aattgtactt
     2641 tcgttcatgt atctgaacca gatgaataat acgttcgtat tgttgaagaa tgactaactc
     2701 ctgtaacggc gtgcgtgggt tccgcagccg ggttcaattt tttttttctt gttcactata
     2761 aaaatatata ataaacgaaa aaaatccgag ggactcaggc tatgaaattt ttcactcaag
     2821 aatttttttt ccttcatcct taattccttt attcactacc caaacccaca atgtcagtta
     2881 aaactaaaac tattacaatt ttaccaggtg atcacgtcgg taccgaaatt gtcaatgaag
     2941 ctatcaaagt tttagaagca attgaagctg ccactcctta ccaaaaaatc catttcgatt
     3001 tcaaacatca tttaatcggt ggtgctgcca ttgatgccac tggtgtccca ttgccagatg
     3061 acgcacttga aagtgccaaa aaatctgatg ccgtcttatt gggtgctgtt ggtggaccaa
     3121 aatgggggac tggcgccgtt cgtccagaac aaggtttatt aaagattcgt aaagaattga
     3181 acctttatgc taacatcaga ccatgtaact ttgccagtga ctccttattg gaattatccc
     3241 cattgagacc agaagtcgtt aaaggtacaa acttgatcat tgttcgtgaa ttagttggtg
     3301 gtatttattt cggtgatcgt gaagaacaag aagaaagtga agataaacaa accgcatggg
     3361 atactgaaaa atacaccgtc gatgaagtca ccagaatcac ccgtatggct gccttcatgg
     3421 ctttacaaca taacccacca ttaccaatct ggtcattgga taaggctaat gttttagctt
     3481 cttctagatt atggagaaga actgtcgata aagtgatttc tgaagagttc ccaactttgt
     3541 ctgttcaaca tcaattgatt gactccgccg ccatgatttt aattcaaaac ccaaccaaat
     3601 tgaatggtat aatcatcact tctaacatgt ttggtgatat catttccgat gaagcttcag
     3661 ttattccagg ttccttgggt ttattaccat ctgcatcttt ggcttcattg ccagatacca
     3721 acaccgcttt tggtctttat gaaccatgtc acggttctgc tcctgattta ccagccaata
     3781 aagttaaccc aattgctact attttatctg ctgcttctat gttgagatta tctttggatt
     3841 gtgtgaaaga agctgaagct ttagaagaag ccgtgaaaca agtcttggat aaaggtatca
     3901 gaactgcaga tttaagaggt accagttcag ctactgaagt tggtgacgcg attgtcgaag
     3961 ctgttactaa aatcttaaaa gaaaaagctt aagtaaagta tacacaaaat ttaacgaata
     4021 ttattaatac aaaatgaaca ccacctaaat actttttttt aaaaatgtac cgactcttct
     4081 aaaaattgcg ataattcaat atcggaaatt tctctcaccg gtttacttgg atcttcggaa
     4141 ataaatctat ttgaagtaat ggtagcctca cttgattctg attggctctt tctagacatg
     4201 ggtaggtaag ggcgaattct gcagatatcc atcacactgg tataacttcg tatagcatac
     4261 attatacgaa gttatggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4321 catgtgagca aaaggcctaa ctagatagca gtcagtcagt caggataacg caggaaagaa
     4381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4861 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4981 tttttttgtt tgcaagcagc agattacg
//
