LOCUS V023248 7792 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023248 VERSION V023248 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 141..157 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 318..697 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 698..901 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 1009..2442 /label="SCARB2(NM_005506)" /note="SCARB2(NM_005506)" /gene="SCARB2" CDS 2458..2484 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2488..2514 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2518..2544 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 3215..3512 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3566..4360 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5012..5146 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" promoter complement(5152..5170) /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" primer_bind complement(5184..5200) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5208..5224 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5232..5262) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5277..5298 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5586..6174) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6345..7205) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7206..7310) /gene="bla" /label="AmpR promoter" rep_origin 7337..7765 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa 961 gatcatgaca tcgattacaa ggatgacgat gacggaggtg gaggaagcat gggccgatgc 1021 tgcttctaca cggcggggac gttgtccctg ctcctgctgg tgaccagcgt cacgctgctg 1081 gtggcccggg tcttccagaa ggctgtagac cagagtatcg agaagaaaat tgtgttaagg 1141 aatggtactg aggcatttga ctcctgggag aagccccctc tgcctgtgta tactcagttc 1201 tatttcttca atgtcaccaa tccagaggag atcctcagag gggagacccc tcgggtggaa 1261 gaagtggggc catacaccta cagggaactc agaaacaaag caaatattca atttggagat 1321 aatggaacaa caatatctgc tgttagcaac aaggcctatg tttttgaacg agaccaatct 1381 gttggagacc ctaaaattga cttaattaga acattaaata ttcctgtatt gactgtcata 1441 gagtggtccc aggtgcactt cctcagggag atcatcgagg ccatgttgaa agcctatcag 1501 cagaagctct ttgtgactca cacagttgac gaattgctct ggggctacaa agatgaaatc 1561 ttgtccctta tccatgtttt caggcccgat atctctccct attttggcct attctatgag 1621 aaaaatggga ctaatgatgg agactatgtt tttctaactg gagaagacag ttaccttaac 1681 tttacaaaaa ttgtggaatg gaatgggaaa acgtcacttg actggtggat aacagacaag 1741 tgcaatatga ttaatggaac agatggagat tcttttcacc cactaataac caaagatgag 1801 gtcctttatg tcttcccatc tgacttttgc aggtcagtgt atattacttt cagtgactat 1861 gagagtgtac agggactgcc tgcctttcgg tataaagttc ctgcagaaat attagccaat 1921 acgtcagaca atgccggctt ctgtatacct gagggaaact gcctgggctc aggagttctg 1981 aatgtcagca tctgcaagaa tggtgcaccc atcattatgt ctttcccaca cttttaccaa 2041 gcagatgaga ggtttgtttc tgccatagaa ggcatgcacc caaatcagga agaccatgag 2101 acatttgtgg acattaatcc tttgactgga ataatcctaa aagcagccaa gaggttccaa 2161 atcaacattt atgtcaaaaa attagatgac tttgttgaaa cgggagacat tagaaccatg 2221 gttttcccag tgatgtacct caatgagagt gttcacattg ataaagagac ggcgagtcga 2281 ctgaagtcta tgattaacac tactttgatc atcaccaaca taccctacat catcatggcg 2341 ctgggtgtgt tctttggttt ggtttttacc tggcttgcat gcaaaggaca gggatccatg 2401 gatgagggaa cagcggatga aagagcaccc ctcattcgaa ccggaggagg gggaagctac 2461 ccatacgacg tacctgatta tgcaacttat ccttatgatg tacctgatta tgctagctat 2521 ccttatgatg tcccagacta cgcataactc gagtggcatc cctgtgaccc ctccccagtg 2581 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 2641 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 2701 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 2761 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 2821 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 2881 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 2941 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 3001 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 3061 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 3121 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 3181 cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3241 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3301 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3361 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3421 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 3481 ctattccaga agtagtgagg aggctttttt ggaggaattg atcagcttgg gatctgatca 3541 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 3601 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 3661 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 3721 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 3781 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 3841 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 3901 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 3961 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 4021 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 4081 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 4141 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 4201 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 4261 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 4321 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 4381 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 4441 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 4501 ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc gagttggttc 4561 agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa atccgtcggc 4621 atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca ggagtgggga 4681 ggcacgatgg ccgctttggt cgacccggac gggacgctcc tgcgcctgat acagaacgaa 4741 ttgcttgcag gcatctcatg agtgtgtctt cccgttttcc gcctgaggtc actgcgtgga 4801 tggagcgctg gcgcctgctg cgcgacggcg agctgctcac cacccactcg ccaagctgga 4861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgccgatca taatcagcca 4921 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 4981 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 5041 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 5101 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaatt ccctatagtg 5161 agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc 5221 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 5281 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 5341 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 5401 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 5461 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 5521 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 5581 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 5641 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 5701 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 5761 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 5821 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 5881 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 5941 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6001 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 6061 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6121 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 6181 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 6241 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 6301 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 6361 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 6421 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 6481 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 6541 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 6601 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 6661 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 6721 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 6781 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 6841 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 6901 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 6961 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7021 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7081 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7141 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 7201 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7261 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7321 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 7381 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 7441 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 7501 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 7561 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 7621 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 7681 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 7741 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat tt //