LOCUS       V034854                 5010 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034854
VERSION     V034854
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(4978..5010,1..72))
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     546..562
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        589..892
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        893..1096
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          1234..1330
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             1394..1420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1448..2686
                     /label="ZDHHC6(NM_001351084)"
                     /note="ZDHHC6(NM_001351084)"
                     /gene="ZDHHC6"
     polyA_signal    2712..2846
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2956..2972)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2980..2996
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3004..3034)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3049..3070
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3358..3946)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4117..4977)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
       61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact
      601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
     1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
     1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg
     1441 aattcccatg ggtacattct gttcggttat caagtttgaa aatctacaag aattaaagag
     1501 actgtgtcac tggggtccca tcatagccct tggtgttata gcaatatgtt ctaccatggc
     1561 catgattgac tctgtgttgt ggtattggcc cttacataca actggaggaa gtgtgaattt
     1621 catcatgttg ataaattgga ctgtcatgat tctttataat tacttcaatg ccatgtttgt
     1681 cggtccgggc tttgtccctc tggggtggaa accggaaatt tctcaggata ccatgtatct
     1741 ccagtattgt aaagtctgcc aagcatacaa ggcaccacgt tcacatcact gcagaaagtg
     1801 taacagatgt gtgatgaaga tggaccatca ctgtccttgg atcaacaact gttgtggtta
     1861 ccaaaatcat gcttcgttca cactgtttct ccttttagca ccactgggtt gtatccatgc
     1921 tgctttcatt tttgtgatga ctatgtacac acagctttat catcggctct cctttgggtg
     1981 gaacacagtg aagatcgaca tgagtgcagc ccggagagat cctcttccaa ttgttccatt
     2041 tggattagct gcatttgcta ccaccttgtt tgccttggga ttagctttag gaacaaccat
     2101 agctgttggg atgttgtttt ttatccagat gaaaataatt ctcagaaaca aaacttctat
     2161 tgagtcatgg attgaagaga aggctaaaga tcgaattcag tattatcaac tagatgaagt
     2221 ctttgttttt ccatatgata tgggaagtag atggaggaac tttaaacagg tatttacgtg
     2281 gtcaggggtc cctgaaggag atggacttga gtggccagta agagaaggct gtcaccaata
     2341 cagcttaaca atagaacagt tgaaacaaaa agcagataag agagtcagaa gtgttcgcta
     2401 taaagtaata gaagattata gtggtgcctg ctgccctctg aataaaggaa tcaaaacctt
     2461 cttcacaagt ccctgcaccg aagagcctcg aatacagctg caaaaagggg aattcatttt
     2521 agccacaaga ggtttacgat actggttata tggagacaaa attcttgatg attcctttat
     2581 agaaggtgtt tcaagaataa ggggttggtt ccctagaaaa tgtgtggaaa agtgtccctg
     2641 tgatgctgaa acagatcaag ccccagaggg ggagaagaaa aatagatagc tcgaggtacc
     2701 gcggccgcgg ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     2761 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2821 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2881 ttcaggggga ggtgtgggag gtttttcgga tcctctagag tcgatctgca ggcatgctag
     2941 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3001 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3061 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3781 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     4921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     4981 cttccttttt caatattatt gaagcattta
//
