LOCUS       pCMV-XRCC1(human)-HA    5660 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031437
VERSION     V031437
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             844..2742
                     /label="XRCC1(NM_006297)"
                     /note="XRCC1(NM_006297)"
                     /gene="XRCC1"
     CDS             2758..2784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2799..2933
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3044..3060)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3068..3084
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3092..3122)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3137..3158
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3446..4034)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4205..5065)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5066..5170)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5644..5660
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc
      841 accatgccgg agatccgcct ccgccatgtc gtgtcctgca gcagccagga ctcgactcac
      901 tgtgcagaaa atcttctcaa ggcagacact taccgaaaat ggcgggcagc caaggcaggc
      961 gagaagacca tctctgtggt cctacagttg gagaaggagg agcagataca cagtgtggac
     1021 attgggaatg atggctcagc tttcgtggag gtgctggtgg gcagttcagc tggaggcgct
     1081 ggggagcaag actatgaggt ccttctggtc acctcatctt tcatgtcccc ttccgagagc
     1141 cgcagtggct caaaccccaa ccgcgttcgc atgtttgggc ctgacaagct ggtccgggca
     1201 gccgccgaga agcgctggga ccgggtcaaa attgtttgca gccagcccta cagcaaggac
     1261 tccccctttg gcttgagttt tgtacggttt catagccccc cagacaaaga tgaggcagag
     1321 gccccgtccc agaaggtgac agtgaccaag cttggccagt tccgtgtgaa ggaggaggat
     1381 gagagcgcca actctctgag gccgggggct ctcttcttca gccggatcaa caagacatcc
     1441 ccagtcacag ccagcgaccc agcaggacct agctatgcag ctgctaccct ccaggcttct
     1501 agtgctgcct cctcagcctc tccagtctcc agggccatag gcagcacctc caagccccag
     1561 gagtctccca aagggaagag gaagttggat ttgaaccaag aagaaaagaa gacccccagc
     1621 aaaccaccag cccagctgtc gccatctgtt cccaagagac ctaaattgcc agctccaact
     1681 cgtaccccag ccacagcccc agtccctgcc cgagcacagg gggcagtgac aggcaaaccc
     1741 cgaggagaag gcaccgagcc cagacgaccc cgagctggcc cagaggagct ggggaagatc
     1801 cttcagggtg tggtagtggt gctgagtggc ttccagaacc ccttccgctc cgagctgcga
     1861 gataaggccc tagagcttgg ggccaagtat cggccagact ggacccggga cagcacgcac
     1921 ctcatctgtg cctttgccaa cacccccaag tacagccagg tcctaggcct gggaggccgc
     1981 atcgtgcgta aggagtgggt gctggactgt caccgcatgc gtcggcggct gccctcccag
     2041 aggtacctca tggcagggcc aggttccagc agtgaggagg atgaggcctc tcacagcggt
     2101 ggcagcggag atgaagcccc caagcttcct cagaagcaac cccagaccaa aaccaagccc
     2161 actcaggcag ctggacccag ctcaccccag aagcccccaa cccctgaaga gaccaaagca
     2221 gcctcaccag tgctccagga agatatagac attgaggggg tacagtcaga aggacaggac
     2281 aatggggcgg aagattctgg ggacacagag gatgagctga ggagggtggc agagcagaag
     2341 gaacacagac tgccccctgg ccaggaggag aatggggaag acccgtatgc aggctccacg
     2401 gatgagaaca cggacagtga ggaacaccag gagcctcctg atctgccagt ccctgagctc
     2461 ccagatttct tccagggcaa gcacttcttt ctttacgggg agttccctgg ggacgagcgg
     2521 cggaaactca tccgatacgt cacagccttc aatggggagc tcgaggacta tatgagtgac
     2581 cgggttcagt ttgtgatcac agcacaggaa tgggatccca gctttgagga ggccctgatg
     2641 gacaacccct ccctggcatt cgttcgtccc cgatggatct acagttgcaa tgagaagcag
     2701 aagttacttc ctcaccagct ctatggggtg gtgccgcaag cctctcgagg taccggctac
     2761 ccatacgatg ttccagatta cgcttaagcg gccgcgggga tccagacatg ataagataca
     2821 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2881 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2941 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcggatc
     3001 ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg
     3061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     5221 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     5281 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5341 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5401 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     5461 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     5521 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     5581 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     5641 gttgtaaaac gacggccagt
//
