LOCUS       V039052                 6203 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039052
VERSION     V039052
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             55..2190
                     /label="USP44(NM_001042403)"
                     /note="USP44(NM_001042403)"
                     /gene="USP44"
     CDS             2248..2499
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
                     /label="VC155"
     polyA_signal    2514..2648
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2759..2775)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2783..2799
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2807..2837)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2852..2873
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3161..3749)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3920..4780)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4781..4885)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5359..5375
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        5402..5705
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        5706..5909
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          6047..6143
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattatgcta
       61 gcaatggata cgtgcaaaca tgttgggcag ctgcagcttg ctcaagacca ttccagcctc
      121 aaccctcaga aatggcactg tgtggactgc aacacgaccg agtccatttg ggcttgcctt
      181 agctgctccc atgttgcctg tggaagatat attgaagagc atgcactcaa gcactttcaa
      241 gaaagcagtc atcctgttgc attggaggtg aatgagatgt acgttttttg ttacctttgt
      301 gatgattatg ttctgaatga taacacaact ggagacctga agttactacg acgtacatta
      361 agtgccatca aaagtcaaaa ttatcactgc acaactcgta gtgggaggtt tttacggtcc
      421 atgggtacag gtgatgattc ttatttctta catgacggtg cccaatctct gcttcaaagt
      481 gaagatcaac tgtatactgc tctttggcac aggagaagga tactaatggg taaaatcttt
      541 cgaacatggt ttgaacaatc acccattgga agaaaaaagc aagaagaacc atttcaggaa
      601 aaaatagtag taaaaagaga agtaaagaaa agacggcagg aattggagta tcaagttaaa
      661 gcagaattgg aaagtatgcc tccaagaaag agtttacgtt tacaagggct cgctcagtcg
      721 accataatag aaatagtttc tgttcaggtg ccagcacaaa cgccagcatc accagcaaaa
      781 gataaagtac tctctacctc agaaaatgaa atatctcaaa aagtcagtga ctcctcagtt
      841 aaacgaaggc caatagtaac tcctggtgta acaggattga gaaatttggg aaatacttgc
      901 tatatgaatt ctgttcttca ggtgttgagt catttactta tttttcgaca atgtttttta
      961 aagcttgatc tgaaccaatg gctggctatg actgctagcg agaagacaag atcttgtaag
     1021 catccaccag tcacagatac agtagtatat caaatgaatg aatgtcagga aaaagataca
     1081 ggttttgttt gctccagaca atcaagtctg tcatcaggac taagtggtgg agcatcaaaa
     1141 ggtagaaaga tggaacttat tcagccaaag gagccaactt cacagtacat ttctctttgt
     1201 catgaattgc atactttgtt ccaagtcatg tggtctggaa agtgggcgtt ggtctcacca
     1261 tttgctatgc tacactcagt gtggagactc attcctgcct ttcgtggtta cgcccaacaa
     1321 gacgctcagg aatttctttg tgaactttta gataaaatac aacgtgaatt agagacaact
     1381 ggtaccagtt taccagctct tatccccact tctcaaagga aactcatcaa acaagttctg
     1441 aatgttgtaa ataacatttt tcatggacaa cttcttagtc aggttacatg tcttgcatgt
     1501 gacaacaaat caaataccat agaacctttc tgggacttgt cattggagtt tccagaaagg
     1561 tatcaatgca gtggaaaaga tattgcttcc cagccatgtc tggttactga aatgttggcc
     1621 aaatttacag aaactgaagc tttagaagga aaaatctacg tatgtgacca gtgtaactca
     1681 aagcgtagaa ggttttcctc caaaccagtt gtactcacag aagcccagaa acaacttatg
     1741 atatgccacc tacctcaggt tctcagactg cacctcaaac gattcaggtg gtcaggacgt
     1801 aataaccgag agaagattgg tgttcatgtt ggctttgagg aaatcttaaa catggagccc
     1861 tattgctgca gggagaccct gaaatccctc agaccagaat gctttatcta tgacttgtcc
     1921 gcggtggtga tgcaccatgg gaaaggattt ggctcagggc actacactgc ctactgctat
     1981 aattctgaag gagggttctg ggtacactgc aatgattcca aactaagcat gtgcactatg
     2041 gatgaagtat gcaaggctca agcttatatc ttgttttata cccaacgagt tactgagaat
     2101 ggacattcta aacttttgcc tccagagctc ctgttgggga gccaacatcc caatgaagac
     2161 gctgatacct cgtctaatga aatccttagc ggtacccgtc cggcgtgcaa aatcccgaac
     2221 gacctgaaac agaaagtcat gaaccacgac aagcagaaga acggcatcaa ggccaacttc
     2281 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     2341 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     2401 aaactgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2461 gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc ggggatccag
     2521 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     2581 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     2641 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     2701 aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt
     2761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3601 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     4921 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     4981 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5041 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5101 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5161 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     5221 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     5281 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     5341 tttcccagtc acgacgttgt aaaacgacgg ccagtgagtt cgagcttgca tgcctgcagg
     5401 tcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     5461 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     5521 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     5581 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     5641 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     5701 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     5761 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5821 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     5881 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     5941 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc
     6001 tagaggatcc ggtactagag gaactgaaaa accagaaagt taactggtaa gtttagtctt
     6061 tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa ctgctcctca
     6121 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc
     6181 ggaattgtac ccgcgggccc acc
//
