LOCUS       V040378                 7912 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040378
VERSION     V040378
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..3040
                     /label="USP10(NM_001272075)"
                     /note="USP10(NM_001272075)"
                     /gene="USP10"
     polyA_signal    3081..3305
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        3321..3844
                     /label="mCMV promoter"
     CDS             3858..4577
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4700..4821
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4828..5283)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5310..5414
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5416..5773
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5808..6602
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6834..6881
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7210..7798
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatgccc tggttgccct ctcctggaat
      661 agggcagtat atttttggag attttagccc tgatgaattc aatcaattct ttgtgactcc
      721 tcgatcttca gttgagcttc ctccatacag tggaacagtt ctgtgtggca cacaggctgt
      781 ggataaacta cctgatggac aagaatatca gagaattgag tttggtgtcg atgaagtcat
      841 tgaacccagt gacactttgc cgagaacccc cagctacagt atttcaagca cactgaaccc
      901 tcaggcccct gaatttattc tcggttgtac agcttccaaa ataacccctg atggtatcac
      961 taaagaagca agctatggct ccatcgactg ccagtaccca ggctctgccc tcgctttgga
     1021 tggaagttct aatgtggagg cggaagtttt ggaaaatgat ggtgtctcag gtggtcttgg
     1081 acaaagggag cgtaaaaaga agaaaaagcg gccacctgga tattacagct atttgaaaga
     1141 tggtggcgat gatagtatct ccacagaagc cctggtcaat ggccatgcca attcagcagt
     1201 cccgaacagt gtcagtgcag aggatgcaga atttatgggt gacatgcccc cgtcagttac
     1261 gcccaggact tgtaacagcc cccagaactc cacagactct gtcagtgaca ttgtgcctga
     1321 cagtcctttc cccggagcac tcggcagtga caccaggact gcagggcagc cagagggggg
     1381 ccccggggct gattttggtc agtcctgctt ccctgcagag gctggcagag acaccctgtc
     1441 aaggacagct ggggctcagc cctgcgttgg taccgatact actgaaaacc ttggagttgc
     1501 taatggacaa atacttgaat cctcgggtga gggcacagct accaacgggg tggagttgca
     1561 caccacggaa agcatagact tggacccaac caaacccgag agtgcatcac ctcctgctga
     1621 cggcacgggc tctgcatcag gcacccttcc tgtcagccag cccaagtcct gggccagcct
     1681 ctttcatgat tctaagccct cttcctcctc gccggtggcc tatgtggaaa ctaagtattc
     1741 ccctcccgcc atatctcccc tggtttctga aaagcaggtt gaagtcaaag aagggcttgt
     1801 tccggtttca gaggatcctg tagccataaa gattgcagag ttgctggaga atgtaaccct
     1861 aatccataaa ccagtgtcgt tgcaaccccg tgggctgatc aataaaggga actggtgcta
     1921 cattaatgct acactgcagg cattggttgc ttgcccgccg atgtaccacc tgatgaagtt
     1981 cattcctctg tattccaaag tgcaaaggcc ttgtacgtca acacccatga tagacagctt
     2041 tgttcggcta atgaatgagt tcactaatat gccagtacct ccaaaacccc gacaagctct
     2101 tggagataaa atcgtgaggg atattcgccc tggagctgcc tttgagccca catatattta
     2161 cagactcctg acagttaaca agtcaagcct gtctgaaaag ggtcgacaag aagatgctga
     2221 ggaatactta ggcttcattc taaatggact tcatgaggaa atgttgaacc taaagaagct
     2281 tctctcacca agtaatgaaa aacttacgat ttccaacggc cccaaaaacc actcggtcaa
     2341 tgaagaagag caggaagaac aaggtgaagg aagcgaggat gaatgggaac aagtgggccc
     2401 ccggaacaag acttccgtca cccgccaggc ggattttgtt cagactccaa tcaccggcat
     2461 ttttggtgga cacatcaggt ctgtggttta ccagcagagt tcaaaagaat ctgccacttt
     2521 gcagccattt ttcacgttgc agttggatat ccagtcagac aagatacgca cagtccagga
     2581 tgcactggag agcttggtgg caagagaatc tgtccaaggt tataccacaa aaaccaaaca
     2641 agaggttgag ataagtcgaa gagtgactct ggaaaaactc cctcctgtcc tcgtgctgca
     2701 cctgaaacga ttcgtttatg agaagactgg tgggtgccag aagcttatca aaaatattga
     2761 atatcctgtg gacttggaaa ttagtaaaga actgctttct ccaggggtta aaaataagaa
     2821 ttttaaatgc caccgaacct atcggctctt tgcagtggtc taccatcacg gcaacagtgc
     2881 gacgggcggc cattacacta cagacgtctt ccagatcggt ctgaatggct ggctgcgcat
     2941 cgatgaccag acagtcaagg tgatcaacca gtaccaggtg gtgaaaccaa ctgctgaacg
     3001 cacagcctac ctcctgtatt accgccgagt ggacctgctg taaggatccg ggcccgttta
     3061 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3121 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3181 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3241 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     3301 tatggtctag agatatactg agtcattagg gactttccaa tgggttttgc ccagtacata
     3361 aggtcaatag gggtgaatca acaggaaagt cccattggag ccaagtacac tgagtcaata
     3421 gggactttcc attgggtttt gcccagtaca aaaggtcaat agggggtgag tcaatgggtt
     3481 tttcccatta ttggcacgta cataaggtca ataggggtga gtcattgggt ttttccagcc
     3541 aatttaatta aaacgccatg tactttccca ccattgacgt caatgggcta ttgaaactaa
     3601 tgcaacgtga cctttaaacg gtactttccc atagctgatt aatgggaaag taccgttctc
     3661 gagccaatac acgtcaatgg gaagtgaaag ggcagccaaa acgtaacacc gccccggttt
     3721 tcccctggaa attccatatt ggcacgcatt ctattggctg agctgcgttc tacgtgggta
     3781 taagaggcgc gaccagcgtc ggtaccgtcg cagtcttcgg tctgaccacc gtagaacgca
     3841 gatcaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3901 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3961 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4021 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4081 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4141 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4201 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4261 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4321 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4381 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4441 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     4501 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4561 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     4621 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     4681 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     4741 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4801 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     4861 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     4921 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     4981 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5041 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5101 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     5161 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     5221 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     5281 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5341 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5401 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     5461 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5521 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5581 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5641 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5701 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5761 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5821 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5881 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5941 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     6001 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6061 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6121 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6181 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6241 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6301 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     6361 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6421 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6481 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6541 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6601 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6661 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6721 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     6781 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     6841 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     6901 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     6961 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     7021 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     7081 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7141 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7261 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7321 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7381 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7441 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7501 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7561 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7621 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7681 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7741 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7801 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7861 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
