LOCUS pCMV-Skp1(mouse)-HA 4262 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045942 VERSION V045942 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 27..330 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 331..534 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 672..768 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" CDS 832..858 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 892..1380 /label="Skp1(NM_011543)" /note="Skp1(NM_011543)" /gene="Skp1" polyA_signal 1401..1535 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(1646..1662) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 1670..1686 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(1694..1724) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 1739..1760 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(2048..2636) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(2807..3667) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(3668..3772) /gene="bla" /label="AmpR promoter" primer_bind 4246..4262 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc 61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag 661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg 721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa 781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac 841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggtcgac catgcctacg 901 ataaagttgc agagttctga tggagagata tttgaagttg atgtagaaat tgccaaacaa 961 tctgtgacta tcaagaccat gctggaagat ttgggaatgg atgatgaagg agatgatgat 1021 cctgttcctt taccaaatgt taatgcagca attctaaaga aggtcattca gtggtgcacc 1081 caccacaaag atgaccctcc tcctcctgag gatgatgaaa acaaagaaaa gcggacagat 1141 gatattcctg tttgggacca agaattcctg aaagttgacc aaggaacact ttttgaactt 1201 attctggctg caaactactt agacatcaaa ggtttgcttg atgtcacatg caagactgtg 1261 gccaatatga ttaaggggaa aactcctgag gagattcgta aaaccttcaa tatcaaaaat 1321 gactttactg aagaggaaga ggcccaggta cgcaaagaga accaatggtg tgaagagaag 1381 tgaggtaccg cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa 1441 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 1501 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 1561 tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca 1621 ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 1681 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 1741 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 1801 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 1861 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 1921 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 1981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 2041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 2101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 2161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 2221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 2281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 2341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 2401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 2461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 2521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 2581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 2641 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 2701 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 2761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 2821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 2881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 2941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 3001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 3061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 3121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 3181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 3241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 3301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 3361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 3421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 3481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 3541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 3601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 3661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 3721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 3781 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 3841 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 3901 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 3961 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 4021 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 4081 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 4141 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 4201 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 4261 gt //