LOCUS V028246 5707 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028246 VERSION V028246 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 937..1458 /label="RBM8A(NM_005105)" /note="RBM8A(NM_005105)" /gene="RBM8A" CDS 1471..1536 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1571..1795 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1841..2269 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2283..2612 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2624..3223 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 3383..3504 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3553..3569) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3577..3593 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3601..3631) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3646..3667 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(3955..4540) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4711..5571) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5572..5676) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 aagctttcta gagaattcga tatcggatcc gccaccatgg cggacgtgct agatcttcac 961 gaggctgggg gcgaagattt cgccatggat gaggatgggg acgagagcat tcacaaactg 1021 aaagaaaaag cgaagaaacg gaagggtcgc ggctttggct ccgaagaggg gtcccgagcg 1081 cggatgcgtg aggattatga cagcgtggag caggatggcg atgaacccgg accacaacgc 1141 tctgttgaag gctggattct ctttgtaact ggagtccatg aggaagccac cgaagaagac 1201 atacacgaca aattcgcaga atatggggaa attaaaaaca ttcatctcaa cctcgacagg 1261 cgaacaggat atctgaaggg gtatactcta gttgaatatg aaacatacaa ggaagcccag 1321 gctgctatgg agggactcaa tggccaggat ttgatgggac agcccatcag cgttgactgg 1381 tgttttgttc ggggtccacc aaaaggcaag aggagaggtg gccgaagacg cagcagaagt 1441 ccagaccgga gacgtcgcct cgagaccggt gactacaaag accatgacgg tgattataaa 1501 gatcatgaca tcgattacaa ggatgacgat gacaagtgac cggtaagttt aaaccgctga 1561 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1621 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1681 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1741 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 1801 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 1861 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1921 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1981 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 2041 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 2101 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 2161 acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggccatttcg 2221 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 2281 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2341 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 2401 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2521 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 2581 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggatgaccg agtacaagcc 2641 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 2701 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 2761 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 2821 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 2881 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 2941 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 3001 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 3061 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 3121 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 3181 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgattcgaaa tgaccgacca 3241 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 3301 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 3361 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 3421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 3481 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 3541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 3601 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 3661 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 3721 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 3781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4381 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt 4501 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 4561 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 4621 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 4681 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 4741 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 4801 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 4861 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 4921 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 4981 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 5041 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 5101 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 5161 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 5221 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 5281 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 5341 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 5401 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 5461 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 5521 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 5581 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 5641 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 5701 tgacgtc //