LOCUS       V047109                11185 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047109
VERSION     V047109
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             925..6936
                     /label="KAT6A(NM_006766)"
                     /note="KAT6A(NM_006766)"
                     /gene="KAT6A"
     CDS             6949..7014
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    7049..7273
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      7319..7747
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7761..8090
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8102..8701
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    8861..8982
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9031..9047)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9055..9071
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9079..9109)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9124..9145
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9433..10018)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10189..11049)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11050..11154)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcgc caccatggta aaactcgcaa acccgcttta tactgagtgg
      961 attttggagg ccatcaaaaa agtgaaaaag cagaaacagc gtccttcaga agaaaggata
     1021 tgcaatgctg tgtcttcatc ccatggcttg gatcgtaaaa ctgttttaga acaattggag
     1081 ttgagtgtta aagatggaac aattttaaaa gtctcaaata aaggactcaa ttcctataaa
     1141 gatcctgata atcctgggcg aatagcactt cctaagcctc ggaaccatgg aaaattggat
     1201 aataaacaaa atgtggattg gaataaactg ataaagcggg cagttgaggg cttggcagag
     1261 tctggtggct caactttgaa aagcattgaa cgttttttga aaggtcagaa ggatgtgtct
     1321 gcattattcg gaggcagtgc tgcctctggc tttcaccagc agttacgatt ggctatcaaa
     1381 cgtgccattg gccacggcag actccttaaa gatggacctc tttatcggct caacactaaa
     1441 gcaaccaacg tggatgggaa agagagttgt gagtctcttt cctgtttacc tccagtgtcc
     1501 cttcttccac atgaaaagga taagccggtt gctgaaccaa tccccatctg tagtttctgt
     1561 cttggtacaa aagaacaaaa ccgagaaaag aagccagagg aactcatctc ctgtgccgac
     1621 tgtggcaaca gtggccatcc atcctgttta aagttttccc ctgaactaac ggttcgagtg
     1681 aaggccttac ggtggcagtg catcgagtgt aaaacatgca gctcctgtcg agatcaaggc
     1741 aaaaatgcgg ataacatgct cttttgtgat tcatgtgacc gaggttttca catggagtgt
     1801 tgtgatccgc cactcacccg tatgccaaaa ggcatgtgga tatgtcaaat atgtcgacct
     1861 aggaaaaaag gacgaaaact tctacaaaag aaggcagcac agataaaacg gcgctatact
     1921 aatccaatag gacgtccaaa aaacaggtta aagaaacaaa acacggtatc aaaaggtccc
     1981 ttcagcaaag ttcgaactgg ccctggaagg ggtaggaaac gaaaaatcac tctttccagc
     2041 caatcagcat catcatcatc agaagaagga tatttagagc ggatagatgg cttggacttc
     2101 tgcagagata gcaatgtctc cttgaagttc aacaagaaaa ccaaagggct cattgatggc
     2161 cttaccaaat tttttacccc ttcccctgat gggcggaaag ctcgggggga agtggtggac
     2221 tactctgagc aatatcgaat cagaaagagg ggcaacagga aatcaagcac ttcagattgg
     2281 cccacagaca atcaggatgg ctgggatggc aaacaagaaa atgaggagcg actttttggg
     2341 agccaggaaa tcatgactga gaaagatatg gaattatttc gtgatatcca agaacaagca
     2401 ctgcagaaag ttggagtgac tggtccccct gatccacaag tccgctgtcc ctctgtcatt
     2461 gagtttggga agtatgaaat tcacacctgg tactcctccc catatcctca agaatactca
     2521 aggctgccca aattgtatct ttgtgaattt tgtctaaaat atatgaaaag tagaactatt
     2581 ctgcagcagc acatgaagaa atgtggttgg ttccatcctc ctgccaatga gatttacaga
     2641 aagaataata tttctgtctt tgaggttgat gggaatgtga gtaccattta ttgtcaaaac
     2701 ctgtgtcttt tggcaaagtt gtttcttgac cacaaaaccc tctattacga tgtggagcca
     2761 tttctttttt atgtactaac acagaatgat gtcaagggct gccaccttgt tggctacttt
     2821 tctaaggaaa agcactgcca acagaagtac aatgtttcct gtataatgat tcttcctcaa
     2881 taccagcgta agggctatgg caggtttctc atcgatttca gttatttgtt atcaaagcgt
     2941 gaaggccaag cagggtctcc agagaaaccg ttatctgatc tgggtcgtct ttcctacatg
     3001 gcatattgga aaagtgtaat attggagtgc ctttatcacc aaaatgacaa gcagatcagc
     3061 attaagaagt taagcaagtt gactggaatc tgccctcaag acatcacttc cacactccac
     3121 cacctacgaa tgctggactt ccgtagtgac caatttgtga ttatccgccg ggaaaaactt
     3181 atccaggatc acatggcaaa gcttcagctg aatttgcgac ctgtagatgt agatccagaa
     3241 tgtttgcgct ggactccagt catagtgtcc aactctgtgg tctcagagga ggaagaagag
     3301 gaggctgagg aaggagaaaa cgaagagcca cagtgccagg aaagagaatt agagatcagt
     3361 gtgggaaagt ctgtgtctca tgagaacaaa gaacaagatt cttattcagt agaaagtgaa
     3421 aagaaaccag aagttatggc tccagtcagt tctacacgtt tgagcaaaca agtccttcct
     3481 catgatagtc ttcctgcaaa tagccagcca tctcggaggg gccgctgggg gaggaagaac
     3541 agaaaaaccc aggaacgttt tggtgataaa gattctaaac tgctcttgga agagacgtct
     3601 tcagctcctc aggaacaata tggagaatgt ggggagaaat cagaagccac ccaggaacaa
     3661 tacactgaaa gtgaagaaca gctggtggct tctgaggagc agccaagcca ggacgggaaa
     3721 cctgaccttc ccaagagaag actcagtgag ggggttgagc cctggcgagg acagctcaag
     3781 aaaagccctg aggctctgaa gtgcagatta acagaaggaa gtgagaggct gccccgtcgc
     3841 tacagtgagg gtgacagggc tgtcctcagg ggcttcagtg agagcagcga ggaggaggag
     3901 gagccggaaa gccctcggtc aagctcgcca ccaattctca caaagcccac gctgaagcga
     3961 aagaaaccat ttctccaccg aaggaggaga gtccgaaagc gcaaacacca caatagcagt
     4021 gtagtcacag aaactatttc tgagaccact gaagtgttag atgaaccttt tgaagattct
     4081 gactccgaga ggccaatgcc aagattagaa cccacgtttg agatcgatga agaagaggag
     4141 gaagaggatg aaaatgaact tttccctaga gaatacttcc gtcgtttgtc ttcgcaggat
     4201 gtactcaggt gtcagtcctc ttctaagagg aagtctaaag atgaagaaga agatgaagag
     4261 tcagatgatg ctgatgacac tcctatctta aagccagtat ctcttttgcg aaaacgtgat
     4321 gtgaagaatt ctcctcttga gccagataca tccacacctt tgaaaaagaa aaagggatgg
     4381 cccaaaggca agagccgcaa accaatccac tggaagaaaa gacctggtcg aaaaccagga
     4441 tttaagttga gtcgggaaat catgccagtt tctactcaag catgcgtcat tgagcccatc
     4501 gtttccattc ctaaagctgg acgtaaaccc aagatccagg agagtgaaga aactgttgag
     4561 ccaaaagaag acatgcccct acccgaggag aggaaggagg aggaggagat gcaagcagag
     4621 gcagaagagg ctgaagaggg tgaggaagag gatgcagcca gcagtgaagt cccagcagcc
     4681 tctccagcag acagcagcaa tagtcctgag accgaaacca aggagcctga ggtggaggag
     4741 gaagaagaga agccccgtgt ctcagaggag cagaggcagt cagaggagga gcagcaggaa
     4801 ttagaggagc cagagccaga ggaggaggaa gatgcagctg cagagactgc ccagaatgac
     4861 gaccacgacg ctgatgatga ggatgatggc cacctggagt ccacaaagaa aaaggagcta
     4921 gaggaacagc ccacgaggga agatgtcaag gaggagcctg gtgttcaaga gtctttttta
     4981 gatgctaata tgcagaagag tagggaaaag ataaaggata aagaggaaac cgagctggat
     5041 tccgaagagg agcagccttc ccatgacacg tccgtggtgt cagagcagat ggctgggtct
     5101 gaggacgacc acgaagaaga ctcccacact aaggaagagt taatcgaatt aaaagaggag
     5161 gaagagattc ctcatagtga gctggatctg gaaactgtac aggcagtgca gtctttgact
     5221 caagaagaaa gcagtgagca tgagggcgcc taccaggact gtgaggaaac tcttgcggcg
     5281 tgtcagaccc tgcagagtta cacccaggct gacgaggacc ctcagatgtc catggttgaa
     5341 gactgtcatg cgtcagaaca taatagccct atctcctccg ttcagtctca ccccagccag
     5401 tcagtccgtt cggtcagcag tcccaacgtg cctgcccttg agagtggcta cacccagatc
     5461 agcccagaac aaggatccct gtccgcaccc tctatgcaga acatggagac cagccccatg
     5521 atggatgtgc cttccgtatc agaccactct cagcaggtgg tggacagcgg cttcagtgac
     5581 ctgggcagca ttgagagcac cactgaaaac tatgagaacc caagcagtta cgactccacg
     5641 atgggcggca gcatctgtgg gaacagctct tcccagagca gctgctccta cggtgggctg
     5701 tcgtcctcca gcagcctcac ccagagcagc tgtgtggtca ctcagcagat ggccagcatg
     5761 ggcagcagct gcagcatgat gcagcagagc agcgtccagc ctgctgccaa ctgcagcatc
     5821 aagtcacctc agagctgcgt ggtggagagg cctcccagta accagcagca gcagccgcca
     5881 ccaccgcctc cacagcagcc acagccgccg ccgccacaac cacaaccagc accacagcct
     5941 ccaccacccc agcagcagcc gcaacagcag ccgcagcctc agccccagca gcctccaccc
     6001 ccaccccctc cccagcagca gcccccgctg tcacagtgta gtatgaataa cagtttcacc
     6061 ccagctccta tgatcatgga gataccagaa tctggaagca ctgggaacat aagtatctat
     6121 gagaggattc caggggattt tggtgccggc agctactctc aaccatcagc caccttcagc
     6181 ctagccaagc tgcagcagct gaccaacacc attatggacc ctcatgccat gccttatagc
     6241 cattctcctg ctgtgacttc ctatgcaacc agtgtttctc tgtccaatac aggactggct
     6301 cagctggctc catctcatcc cttagctggg actcctcaag cacaagccac catgacgcca
     6361 cccccaaact tggcatccac taccatgaac ctcacatctc ctctgcttca gtgcaacatg
     6421 tctgccacca acattggcat tcctcacacg cagagattgc aagggcaaat gccagtgaag
     6481 gggcacattt ccatccgctc caagtctgcg ccactgccct ctgcggctgc tcaccagcag
     6541 cagctgtatg gccgtagccc atcggcagtt gccatgcagg ctggccctcg cgcactggct
     6601 gttcagcgtg gcatgaacat gggggttaat ctgatgccta ctcccgccta taatgtcaat
     6661 tccatgaata tgaacacctt gaatgccatg aacagctatc gaatgacaca gcccatgatg
     6721 aacagcagtt accatagtaa ccctgcctac atgaaccaga cagcacagta tcctatgcag
     6781 atgcagatgg gaatgatggg gagccaggcc tatacccagc agcctatgca gcctaaccct
     6841 catgggaaca tgatgtacac aggcccctcc catcacagct acatgaacgc tgctggcgtg
     6901 cccaagcagt cactcaacgg accttacatg agaagactcg agaccggtga ctacaaagac
     6961 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagtgaccg
     7021 gtaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7081 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7141 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7201 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7261 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     7321 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7381 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7441 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7501 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7561 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7621 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7681 gggattttgg ccatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7741 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7801 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7861 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7921 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7981 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     8041 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     8101 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     8161 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     8221 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8281 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     8341 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8401 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8461 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8521 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8581 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8641 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     8701 attcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     8761 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     8821 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     8881 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8941 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9001 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9061 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9121 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9181 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9241 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9841 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9901 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9961 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10021 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10081 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10141 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10201 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10261 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10321 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10381 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10441 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10501 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10561 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10621 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10681 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10741 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10801 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10861 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10921 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10981 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11041 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11101 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11161 tccccgaaaa gtgccacctg acgtc
//
