LOCUS V039565 5932 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039565 VERSION V039565 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 925..1683 /label="C1QB(NM_000491)" /note="C1QB(NM_000491)" /gene="C1QB" CDS 1696..1761 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1796..2020 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2066..2494 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2508..2837 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2849..3448 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 3608..3729 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3778..3794) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3802..3818 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3826..3856) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3871..3892 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4180..4765) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4936..5796) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5797..5901) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 aagctttcta gagaattcgc caccatgatg atgaagatcc catggggcag catcccagta 961 ctgatgttgc tcctgctcct gggcctaatc gatatctccc aggcccagct cagctgcacc 1021 gggcccccag ccatccctgg catcccgggt atccctggga cacctggccc cgatggccaa 1081 cctgggaccc cagggataaa aggagagaaa gggcttccag ggctggctgg agaccatggt 1141 gagttcggag agaagggaga cccagggatt cctgggaatc caggaaaagt cggccccaag 1201 ggccccatgg gccctaaagg tggcccaggg gcccctggag ccccaggccc caaaggtgaa 1261 tcgggagact acaaggccac ccagaaaatc gccttctctg ccacaagaac catcaacgtc 1321 cccctgcgcc gggaccagac catccgcttc gaccacgtga tcaccaacat gaacaacaat 1381 tatgagcccc gcagtggcaa gttcacctgc aaggtgcccg gtctctacta cttcacctac 1441 cacgccagct ctcgagggaa cctgtgcgtg aacctcatgc gtggccggga gcgtgcacag 1501 aaggtggtca ccttctgtga ctatgcctac aacaccttcc aggtcaccac cggtggcatg 1561 gtcctcaagc tggagcaggg ggagaacgtc ttcctgcagg ccaccgacaa gaactcacta 1621 ctgggcatgg agggtgccaa cagcatcttt tccgggttcc tgctctttcc agatatggag 1681 gccctcgaga ccggtgacta caaagaccat gacggtgatt ataaagatca tgacatcgat 1741 tacaaggatg acgatgacaa gtgaccggta agtttaaacc gctgatcagc ctcgactgtg 1801 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 1861 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 1921 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 1981 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 2041 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 2101 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 2161 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 2221 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 2281 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 2341 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 2401 atctcggtct attcttttga tttataaggg attttggcca tttcggccta ttggttaaaa 2461 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 2521 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2581 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2641 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 2701 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 2761 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 2821 gcctaggctt ttgcaaaaag ctcccgggat gaccgagtac aagcccacgg tgcgcctcgc 2881 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 2941 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 3001 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 3061 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 3121 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 3181 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 3241 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 3301 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 3361 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 3421 gtgcatgacc cgcaagcccg gtgcctgatt cgaaatgacc gaccaagcga cgcccaacct 3481 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 3541 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 3601 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 3661 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 3721 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 3781 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 3841 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 3901 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 3961 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 4021 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 4081 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 4141 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 4201 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 4261 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 4321 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 4381 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4441 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4501 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4561 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4621 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4681 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat 4741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 4801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 4861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 4921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 4981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 5041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 5101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 5161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 5221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 5281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 5341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 5401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 5461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 5521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 5581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 5641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 5701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 5761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 5821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 5881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //