LOCUS V025751 7677 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025751 VERSION V025751 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 67..370 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 371..574 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 620..638 /note="promoter for bacteriophage T3 RNA polymerase" /label="T3 promoter" regulatory 669..678 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 678..719 /codon_start=1 /product="epitope tag from simian virus 5" /transl_table=1 /translation="GKPIPNPLLGLDST" /label="V5 tag" CDS 726..4082 /label="WWC1(NM_001161661)" /note="WWC1(NM_001161661)" /gene="WWC1" promoter complement(4179..4197) /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" polyA_signal 4471..4592 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4599..5054) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5081..5185 /gene="bla" /label="AmpR promoter" promoter 5187..5544 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5579..6373 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6605..6652 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6981..7569 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacg 661 cggtggcggc cgccatgggc aagccgatcc ctaaccctct gctgggcctg gacagcactg 721 gatccatgcc ccggccggag ctgcccctgc cggagggctg ggaggaggcg cgcgacttcg 781 acggcaaggt ctactacata gaccacacga accgcaccac cagctggatc gacccgcggg 841 acaggtacac caaaccgctc acctttgctg actgcattag tgatgagttg ccgctaggat 901 gggaagaggc atatgaccca caggttggag attacttcat agaccacaac accaaaacca 961 ctcagattga ggatcctcga gtacaatggc ggcgggagca ggaacatatg ctgaaggatt 1021 acctggtggt ggcccaggag gctctgagtg cacaaaagga gatctaccag gtgaagcagc 1081 agcgcctgga gcttgcacag caggagtacc agcaactgca tgccgtctgg gagcataagc 1141 tgggctccca ggtcagcttg gtctctggtt catcatccag ctccaagtat gaccctgaga 1201 tcctgaaagc tgaaattgcc actgcaaaat cccgggtcaa caagctgaag agagagatgg 1261 ttcacctcca gcacgagctg cagttcaaag agcgtggctt tcagaccctg aagaaaatcg 1321 ataagaaaat gtctgatgct cagggcagct acaaactgga tgaagctcag gctgtcttga 1381 gagaaacaaa agccatcaaa aaggctatta cctgtgggga aaaggaaaag caagatctca 1441 ttaagagcct tgccatgttg aaggacggct tccgcactga cagggggtct cactcagacc 1501 tgtggtccag cagcagctct ctggagagtt cgagtttccc gctaccgaaa cagtacctgg 1561 atgtgagctc ccagacagac atctcgggaa gcttcggcat caacagcaac aatcagttgg 1621 cagagaaggt cagattgcgc cttcgatatg aagaggctaa gagaaggatc gccaacctga 1681 agatccagct ggccaagctt gacagtgagg cctggcctgg ggtgctggac tcagagaggg 1741 accggctgat ccttatcaac gagaaggagg agctgctgaa ggagatgcgc ttcatcagcc 1801 cccgcaagtg gacccagggg gaggtggagc agctggagat ggcccggaag cggctggaaa 1861 aggacctgca ggcagcccgg gacacccaga gcaaggcgct gacggagagg ttaaagttaa 1921 acagtaagag gaaccagctt gtgagagaac tggaggaagc cacccggcag gtggcaactc 1981 tgcactccca gctgaaaagt ctctcaagca gcatgcagtc cctgtcctca ggcagcagcc 2041 ccggatccct cacgtccagc cggggctccc tggttgcatc cagcctggac tcctccactt 2101 cagccagctt cactgacctc tactatgacc cctttgagca gctggactca gagctgcaga 2161 gcaaggtgga gttcctgctc ctggaggggg ccaccggctt ccggccctca ggctgcatca 2221 ccaccatcca cgaggatgag gtggccaaga cccagaaggc agagggaggt ggccgcctgc 2281 aggctctgcg ttccctgtct ggcaccccaa agtccatgac ctccctatcc ccacgttcct 2341 ctctctcctc cccctcccca ccctgttccc ctctcatggc tgaccccctc ctggctggtg 2401 atgccttcct caactccttg gagtttgaag acccggagct gagtgccact ctttgtgaac 2461 tgagccttgg taacagcgcc caggaaagat accggctgga ggaaccagga acggagggca 2521 agcagctggg ccaagctgtg aatacggccc aggggtgtgg cctgaaagtg gcctgtgtct 2581 cagccgccgt atcggacgag tcagtggctg gagacagtgg tgtgtacgag gcttccgtgc 2641 agagactggg tgcttcagaa gctgctgcat ttgacagtga cgaatcggaa gcagtgggtg 2701 cgacccgaat tcagattgcc ctgaagtatg atgagaagaa taagcaattt gcaatattaa 2761 tcatccagct gagtaacctt tctgctctgt tgcagcaaca agaccagaaa gtgaatatcc 2821 gcgtggctgt ccttccttgc tctgaaagca caacctgcct gttccggacc cggcctctgg 2881 acgcctcaga cactctagtg ttcaatgagg tgttctgggt atccatgtcc tatccagccc 2941 ttcaccagaa gaccttaaga gtcgatgtct gtaccaccga caggagccat ctggaagagt 3001 gcctgggagg cgcccagatc agcctggcgg aggtctgccg gtctggggag aggtcgactc 3061 gctggtacaa ccttctcagc tacaaatact tgaagaaaca gagcagggag ctcaagccag 3121 tgggagtcat ggcccctgcc tcagggcctg ccagcacgga cgctgtgtct gctctgttgg 3181 aacagacagc agtggagctg gagaagaggc aggagggcag gagcagcaca cagacactgg 3241 aagacagctg gaggtatgag gagaccagtg agaatgaggc agtagccgag gaagaggagg 3301 aggaggtgga ggaggaggag ggagaagagg atgttttcac cgagaaagcc tcacctgata 3361 tggatgggta cccagcatta aaggtggaca aagagaccaa cacggagacc ccggccccat 3421 cccccacagt ggtgcgacct aaggaccgga gagtgggcac cccgtcccag gggccatttc 3481 ttcgagggag caccatcatc cgctctaaga ccttctcccc aggaccccag agccagtacg 3541 tgtgccggct gaatcggagt gatagtgaca gctccactct gtccaaaaag ccaccttttg 3601 ttcgaaactc cctggagcga cgcagcgtcc ggatgaagcg gccgtcccca cccccacagc 3661 cttcctcggt caagtcgctg cgctccgagc gtctgatccg tacctcgctg gacctggagt 3721 tagacctgca ggcgacaaga acctggcaca gccaattgac ccaggagatc tcggtgctga 3781 aggagctcaa ggagcagctg gaacaagcca agagccacgg ggagaaggag ctgccacagt 3841 ggttgcgtga ggacgagcgt ttccgcctgc tgctgaggat gctggagaag cggcagatgg 3901 accgagcgga gcacaagggt gagcttcaga cagacaagat gatgagggca gctgccaagg 3961 atgtgcacag gctccgaggc cagagctgta aggaaccccc agaagttcag tctttcaggg 4021 agaagatggc atttttcacc cggcctcgga tgaatatccc agctctctct gcagatgacg 4081 tctaagaatt cgatatcgtc gacagatctc tcgagtctag aactagtggg cccggtacct 4141 taattaatta aggtaccagg taagtgtacc caattcgccc tatagtgagt cgtattacaa 4201 ttcactcgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga gatccaattt 4261 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattcacagt 4321 cccaaggctc atttcaggcc cctcagtcct cacagtctgt tcatgatcat aatcagccat 4381 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 4441 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 4501 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 4561 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 4621 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 4681 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 4741 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 4801 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 4861 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 4921 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 4981 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 5041 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 5101 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 5161 tcaataatat tgaaaaagga agaatcctga ggcggaaaga accagctgtg gaatgtgtgt 5221 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 5281 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 5341 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 5401 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 5461 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 5521 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 5581 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 5641 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 5701 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 5761 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 5821 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 5881 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 5941 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 6001 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 6061 acatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 6121 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 6181 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 6241 agcgttggct acccgtgata ttgctgaaga acttggcggc gaatgggctg accgcttcct 6301 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 6361 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 6421 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 6481 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 6541 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 6601 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 6661 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 6721 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 6781 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 6841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 6901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 6961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 7021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 7081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 7141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 7201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 7261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 7321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 7381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 7441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 7501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 7561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 7621 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgcc //