LOCUS V019236 5475 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019236 VERSION V019236 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..1354 /label="Tspan4(NM_053082)" /note="Tspan4(NM_053082)" /gene="Tspan4" CDS 1421..2140 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2263..2384 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2391..2846) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2873..2977 /gene="bla" /label="AmpR promoter" promoter 2979..3336 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3371..4165 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4397..4444 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4773..5361 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atggcgcgcg gctgcctcca 661 gggcgtcaag tacctcatgt tcgccttcaa cctgctcttc tggctgggtg gctgtggtgt 721 cctgggtgtt ggcatctggt tggctgccac acagggaaac tttgccacct tatcatcctc 781 atttccatcc ttgtcggctg ccaacctgct catcgtcacc gggaccttcg tcatggccat 841 cggcttcgtg ggctgcattg gggccctcaa ggagaacaag tgcctactgc tcactttctt 901 tgtgctgctg ctgctagtgt tcctgctgga agccaccatt gctgtgctct tctttgccta 961 cagtgacaag attgacagtt atgcccaaca agacctgaag aagggcctgc atctgtatgg 1021 cacacagggc aacgtgggcc tcaccaatgc ctggagcatc atccagactg atttccgatg 1081 ctgtggagtt tccaattaca ctgattggtt tgaggtatac aatgccactc gtgtgcctga 1141 ctcctgctgt ctggagttca gtgatagctg tgggttacat gaacctggta cctggtggaa 1201 gtcgccctgt tatgagacag tgaaggcctg gctccaggag aacctgctag ctgtgggcat 1261 ctttggactg tgcacggcac tggtgcagat tctgggcctc accttcgcta tgaccatgta 1321 ctgccaggtg gtaaaggcgg acacctactg tgcaggcggc ggagggagtg gaggaggggg 1381 aagcggaggc ggagggagta cggatccacc ggtcgccacc atggtgagca agggcgagga 1441 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 1501 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 1561 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 1621 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 1681 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 1741 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 1801 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 1861 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 1921 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 1981 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 2041 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 2101 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc 2161 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 2221 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 2281 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 2341 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag 2401 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 2461 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 2521 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 2581 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 2641 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 2701 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 2761 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 2821 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac 2881 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 2941 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg 3001 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3061 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3181 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3241 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 3301 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg 3361 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3421 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3481 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3541 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3601 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3661 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3721 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3781 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3841 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag 3901 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 3961 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4021 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4081 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4141 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4201 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4261 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4321 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa 4381 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt 4441 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc 4501 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt 4561 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac 4621 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 4681 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 4741 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 4801 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 4861 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 4921 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 4981 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 5041 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 5101 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 5161 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 5221 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 5281 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 5341 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 5401 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 5461 attaccgcca tgcat //