LOCUS       V039337                 6405 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039337
VERSION     V039337
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2293
                     /label="RELA(NM_021975)"
                     /note="RELA(NM_021975)"
                     /gene="RELA"
     CDS             2351..3070
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    3193..3314
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3321..3776)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3803..3907
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3909..4266
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4301..5095
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5327..5374
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5703..6291
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggacgaac tgttccccct
      661 catcttcccg gcagagccag cccaggcctc tggcccctat gtggagatca ttgagcagcc
      721 caagcagcgg ggcatgcgct tccgctacaa gtgcgagggg cgctccgcgg gcagcatccc
      781 aggcgagagg agcacagata ccaccaagac ccaccccacc atcaagatca atggctacac
      841 aggaccaggg acagtgcgca tctccctggt caccaaggac cctcctcacc ggcctcaccc
      901 ccacgagctt gtaggaaagg actgccggga tggcttctat gaggctgagc tctgcccgga
      961 ccgctgcatc cacagtttcc agaacctggg aatccagtgt gtgaagaagc gggacctgga
     1021 gcaggctatc agtcagcgca tccagaccaa caacaacccc ttccaagttc ctatagaaga
     1081 gcagcgtggg gactacgacc tgaatgctgt gcggctctgc ttccaggtga cagtgcggga
     1141 cccatcaggc aggcccctcc gcctgccgcc tgtcctttct catcccatct ttgacaatcg
     1201 tgcccccaac actgccgagc tcaagatctg ccgagtgaac cgaaactctg gcagctgcct
     1261 cggtggggat gagatcttcc tactgtgtga caaggtgcag aaagaggaca ttgaggtgta
     1321 tttcacggga ccaggctggg aggcccgagg ctccttttcg caagctgatg tgcaccgaca
     1381 agtggccatt gtgttccgga cccctcccta cgcagacccc agcctgcagg ctcctgtgcg
     1441 tgtctccatg cagctgcggc ggccttccga ccgggagctc agtgagccca tggaattcca
     1501 gtacctgcca gatacagacg atcgtcaccg gattgaggag aaacgtaaaa ggacatatga
     1561 gaccttcaag agcatcatga agaagagtcc tttcagcgga cccaccgacc cccggcctcc
     1621 acctcgacgc attgctgtgc cttcccgcag ctcagcttct gtccccaagc cagcacccca
     1681 gccctatccc tttacgtcat ccctgagcac catcaactat gatgagtttc ccaccatggt
     1741 gtttccttct gggcagatca gccaggcctc ggccttggcc ccggcccctc cccaagtcct
     1801 gccccaggct ccagcccctg cccctgctcc agccatggta tcagctctgg cccaggcccc
     1861 agcccctgtc ccagtcctag ccccaggccc tcctcaggct gtggccccac ctgcccccaa
     1921 gcccacccag gctggggaag gaacgctgtc agaggccctg ctgcagctgc agtttgatga
     1981 tgaagacctg ggggccttgc ttggcaacag cacagaccca gctgtgttca cagacctggc
     2041 atccgtcgac aactccgagt ttcagcagct gctgaaccag ggcatacctg tggcccccca
     2101 cacaactgag cccatgctga tggagtaccc tgaggctata actcgcctag tgacaggggc
     2161 ccagaggccc cccgacccag ctcctgctcc actgggggcc ccggggctcc ccaatggcct
     2221 cctttcagga gatgaagact tctcctccat tgcggacatg gacttctcag ccctgctgag
     2281 tcagatcagc tccgaattcg gtggaggcgg atcaggaggc ggtggatctc cggatccacc
     2341 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2401 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2461 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2521 ctggcccacc ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga
     2581 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2641 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2701 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2761 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     2821 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2881 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2941 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3001 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3061 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     3121 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3181 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3241 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3301 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3361 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3421 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3481 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3541 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3601 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3661 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3721 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     3781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3901 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     3961 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4021 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4081 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4141 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4201 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4261 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4321 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4381 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4441 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     4501 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4561 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4621 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4681 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4741 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4801 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     4861 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4921 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4981 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5041 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5101 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5161 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5221 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     5281 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     5341 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5401 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     5461 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     5521 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     5581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5821 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6361 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
