LOCUS V026789 5650 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026789 VERSION V026789 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 657..1574 /label="PABPN1(NM_004643)" /note="PABPN1(NM_004643)" /gene="PABPN1" CDS 1596..2315 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2438..2559 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2566..3021) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3048..3152 /gene="bla" /label="AmpR promoter" promoter 3154..3511 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3546..4340 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4572..4619 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4948..5536 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gccaccatgg 661 cggcggcggc ggcggcggca gcagcagcgg gggctgcggg cggtcggggc tccgggccgg 721 ggcggcggcg ccatcttgtg cccggggccg gtggggaggc cggggagggg gccccggggg 781 gcgcagggga ctacgggaac ggcctggagt ctgaggaact ggagcctgag gagctgctgc 841 tggagcccga gccggagccc gagcccgaag aggagccgcc ccggccccgc gcccccccgg 901 gagctccggg ccctgggcct ggttcgggag cccccggcag ccaagaggag gaggaggagc 961 cgggactggt cgagggtgac ccgggggacg gcgccattga ggacccggag ctggaagcta 1021 tcaaagctcg agtcagggag atggaggaag aagctgagaa gctaaaggag ctacagaacg 1081 aggtagagaa gcagatgaat atgagtccac ctccaggcaa tgctggcccg gtgatcatgt 1141 ccattgagga gaagatggag gctgatgccc gttccatcta tgttggcaat gtggactatg 1201 gtgcaacagc agaagagctg gaagctcact ttcatggctg tggttcagtc aaccgtgtta 1261 ccatactgtg tgacaaattt agtggccatc ccaaagggtt tgcgtatata gagttctcag 1321 acaaagagtc agtgaggact tccttggcct tagatgagtc cctatttaga ggaaggcaaa 1381 tcaaggtgat cccaaaacga accaacagac caggcatcag cacaacagac cggggttttc 1441 cacgagcccg ctaccgcgcc cggaccacca actacaacag ctcccgctct cgattctaca 1501 gtggttttaa cagcaggccc cggggtcgcg tctacagggg ccgggctaga gcgacatcat 1561 ggtattcccc ttacacggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt 1621 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 1681 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 1741 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 1801 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 1861 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 1921 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 1981 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 2041 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 2101 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 2161 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 2221 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 2281 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat 2341 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 2401 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 2461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 2521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta 2581 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 2641 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 2701 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 2761 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 2821 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 2881 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 2941 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 3001 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 3061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 3121 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 3181 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 3241 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 3301 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 3361 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 3421 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 3481 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 3541 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 3601 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 3661 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 3721 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 3781 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 3841 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 3901 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 3961 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 4021 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 4081 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 4141 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 4201 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 4261 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 4321 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 4381 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 4441 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 4501 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 4561 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 4621 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 4681 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 4741 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 4801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 4861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 4921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 4981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 5041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 5101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 5161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 5221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 5281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 5341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 5401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 5461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 5521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 5581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 5641 cgccatgcat //