LOCUS       V036563                 6782 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036563
VERSION     V036563
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             644..664
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             665..685
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             686..706
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             722..1429
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="photostable monomeric orange derivative of DsRed
                     fluorescent protein (Shaner et al., 2008)"
                     /transl_table=1
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mOrange2"
     CDS             1466..3373
                     /label="PABPC1(NM_002568)"
                     /note="PABPC1(NM_002568)"
                     /gene="PABPC1"
     CDS             3380..3445
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3570..3691
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3698..4153)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4180..4284
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4286..4643
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4678..5472
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5704..5751
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6080..6668
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgccaaaaa agaagagaaa
      661 ggtaccaaaa aagaagagaa aggtaccaaa aaagaagaga aaggtacggg atccaccggt
      721 catggtgagc aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa
      781 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      841 ccgcccctac gagggctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc
      901 cttcgcctgg gacatcctgt cccctcattt cacctacggc tccaaggcct acgtgaagca
      961 ccccgccgac atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg
     1021 cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1081 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1141 gatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     1201 tgccctgaag ggcaagatca agatgaggct gaagctgaag gacggcggcc actacacctc
     1261 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt
     1321 cgacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     1381 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gtggcggtgg
     1441 atcaggcggt ggaggatcta ctagtatgaa ccccagtgcc cccagctacc ccatggcctc
     1501 gctctacgtg ggggacctcc accccgacgt gaccgaggcg atgctctacg agaagttcag
     1561 cccggccggg cccatcctct ccatccgggt ctgcagggac atgatcaccc gccgctcctt
     1621 gggctacgcg tatgtgaact tccagcagcc ggcggacgcg gagcgtgctt tggacaccat
     1681 gaattttgat gttataaagg gcaagccagt acgcatcatg tggtctcagc gtgatccatc
     1741 acttcgcaaa agtggagtag gcaacatatt cattaaaaat ctggacaaat ccattgataa
     1801 taaagcactg tatgatacat tttctgcttt tggtaacatc ctttcatgta aggtggtttg
     1861 tgatgaaaat ggttccaagg gctatggatt tgtacacttt gagacgcagg aagcagctga
     1921 aagagctatt gaaaaaatga atggaatgct cctaaatgat cgcaaagtat ttgttggacg
     1981 atttaagtct cgtaaagaac gagaagctga acttggagct agggcaaaag aattcaccaa
     2041 tgtttacatc aagaattttg gagaagacat ggatgatgag cgccttaagg atctctttgg
     2101 caagtttggg cctgccttaa gtgtgaaagt aatgactgat gaaagtggaa aatccaaagg
     2161 atttggattt gtaagctttg aaaggcatga agatgcacag aaagctgtgg atgagatgaa
     2221 cggaaaggag ctcaatggaa aacaaattta tgttggtcga gctcagaaaa aggtggaacg
     2281 gcagacggaa cttaagcgca aatttgaaca gatgaaacaa gataggatca ccagatacca
     2341 gggtgttaat ctttatgtga aaaatcttga tgatggtatt gatgatgaac gtctccggaa
     2401 agagttttct ccatttggta caatcactag tgcaaaggtt atgatggagg gtggtcgcag
     2461 caaagggttt ggttttgtat gtttctcctc cccagaagaa gccactaaag cagttacaga
     2521 aatgaacggt agaattgtgg ccacaaagcc attgtatgta gctttagctc agcgcaaaga
     2581 agagcgccag gctcacctca ctaaccagta tatgcagaga atggcaagtg tacgagctgt
     2641 tcccaaccct gtaatcaacc cctaccagcc agcacctcct tcaggttact tcatggcagc
     2701 tatcccacag actcagaacc gtgctgcata ctatcctcct agccaaattg ctcaactaag
     2761 accaagtcct cgctggactg ctcagggtgc cagacctcat ccattccaaa atatgcccgg
     2821 tgctatccgc ccagctgctc ctagaccacc atttagtact atgagaccag cttcttcaca
     2881 ggttccacga gtcatgtcaa cacagcgtgt tgctaacaca tcaacacaga caatgggtcc
     2941 acgtcctgca gctgcagccg ctgcagctac tcctgctgtc cgcaccgttc cacagtataa
     3001 atatgctgca ggagttcgca atcctcagca acatcttaat gcacagccac aagttacaat
     3061 gcaacagcct gctgttcatg tacaaggtca ggaacctttg actgcttcca tgttggcatc
     3121 tgcccctcct caagagcaaa agcaaatgtt gggtgaacgg ctgtttcctc ttattcaagc
     3181 catgcaccct actcttgctg gtaaaatcac tggcatgttg ttggagattg ataattcaga
     3241 acttcttcat atgctcgagt ctccagagtc actccgttct aaggttgatg aagctgtagc
     3301 tgtactacaa gcccaccaag ctaaagaggc tgcccagaaa gcagttaaca gtgccaccgg
     3361 tgttccaact gttgatatcg actacaaaga ccatgacggt gattataaag atcatgacat
     3421 cgactacaag gatgacgatg acaagtaggc ggccgcgact ctagatcata atcagccata
     3481 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3541 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3661 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
     3721 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     3781 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     3841 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     3901 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     3961 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     4021 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     4081 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     4141 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4201 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4261 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     4321 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4381 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4441 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4501 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4561 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     4621 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
     4681 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4741 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4801 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     4861 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4921 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4981 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5041 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5101 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5161 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     5221 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5281 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5341 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5401 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5461 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5521 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5581 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5641 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     5701 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
     5761 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5821 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5881 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
     5941 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6001 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6061 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
     6781 at
//
