LOCUS V036563 6782 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036563 VERSION V036563 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 644..664 /codon_start=1 /locus_tag="" /note="" /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /protein_id="" /transl_table=1 /translation="PKKKRKV" /label="SV40 NLS" CDS 665..685 /codon_start=1 /locus_tag="" /note="" /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /protein_id="" /transl_table=1 /translation="PKKKRKV" /label="SV40 NLS" CDS 686..706 /codon_start=1 /locus_tag="" /note="" /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /protein_id="" /transl_table=1 /translation="PKKKRKV" /label="SV40 NLS" CDS 722..1429 /codon_start=1 /note="mammalian codon-optimized" /product="photostable monomeric orange derivative of DsRed fluorescent protein (Shaner et al., 2008)" /transl_table=1 /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mOrange2" CDS 1466..3373 /label="PABPC1(NM_002568)" /note="PABPC1(NM_002568)" /gene="PABPC1" CDS 3380..3445 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3570..3691 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3698..4153) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4180..4284 /gene="bla" /label="AmpR promoter" promoter 4286..4643 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4678..5472 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5704..5751 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6080..6668 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgccaaaaa agaagagaaa 661 ggtaccaaaa aagaagagaa aggtaccaaa aaagaagaga aaggtacggg atccaccggt 721 catggtgagc aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa 781 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 841 ccgcccctac gagggctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc 901 cttcgcctgg gacatcctgt cccctcattt cacctacggc tccaaggcct acgtgaagca 961 ccccgccgac atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg 1021 cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 1081 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 1141 gatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 1201 tgccctgaag ggcaagatca agatgaggct gaagctgaag gacggcggcc actacacctc 1261 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt 1321 cgacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 1381 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gtggcggtgg 1441 atcaggcggt ggaggatcta ctagtatgaa ccccagtgcc cccagctacc ccatggcctc 1501 gctctacgtg ggggacctcc accccgacgt gaccgaggcg atgctctacg agaagttcag 1561 cccggccggg cccatcctct ccatccgggt ctgcagggac atgatcaccc gccgctcctt 1621 gggctacgcg tatgtgaact tccagcagcc ggcggacgcg gagcgtgctt tggacaccat 1681 gaattttgat gttataaagg gcaagccagt acgcatcatg tggtctcagc gtgatccatc 1741 acttcgcaaa agtggagtag gcaacatatt cattaaaaat ctggacaaat ccattgataa 1801 taaagcactg tatgatacat tttctgcttt tggtaacatc ctttcatgta aggtggtttg 1861 tgatgaaaat ggttccaagg gctatggatt tgtacacttt gagacgcagg aagcagctga 1921 aagagctatt gaaaaaatga atggaatgct cctaaatgat cgcaaagtat ttgttggacg 1981 atttaagtct cgtaaagaac gagaagctga acttggagct agggcaaaag aattcaccaa 2041 tgtttacatc aagaattttg gagaagacat ggatgatgag cgccttaagg atctctttgg 2101 caagtttggg cctgccttaa gtgtgaaagt aatgactgat gaaagtggaa aatccaaagg 2161 atttggattt gtaagctttg aaaggcatga agatgcacag aaagctgtgg atgagatgaa 2221 cggaaaggag ctcaatggaa aacaaattta tgttggtcga gctcagaaaa aggtggaacg 2281 gcagacggaa cttaagcgca aatttgaaca gatgaaacaa gataggatca ccagatacca 2341 gggtgttaat ctttatgtga aaaatcttga tgatggtatt gatgatgaac gtctccggaa 2401 agagttttct ccatttggta caatcactag tgcaaaggtt atgatggagg gtggtcgcag 2461 caaagggttt ggttttgtat gtttctcctc cccagaagaa gccactaaag cagttacaga 2521 aatgaacggt agaattgtgg ccacaaagcc attgtatgta gctttagctc agcgcaaaga 2581 agagcgccag gctcacctca ctaaccagta tatgcagaga atggcaagtg tacgagctgt 2641 tcccaaccct gtaatcaacc cctaccagcc agcacctcct tcaggttact tcatggcagc 2701 tatcccacag actcagaacc gtgctgcata ctatcctcct agccaaattg ctcaactaag 2761 accaagtcct cgctggactg ctcagggtgc cagacctcat ccattccaaa atatgcccgg 2821 tgctatccgc ccagctgctc ctagaccacc atttagtact atgagaccag cttcttcaca 2881 ggttccacga gtcatgtcaa cacagcgtgt tgctaacaca tcaacacaga caatgggtcc 2941 acgtcctgca gctgcagccg ctgcagctac tcctgctgtc cgcaccgttc cacagtataa 3001 atatgctgca ggagttcgca atcctcagca acatcttaat gcacagccac aagttacaat 3061 gcaacagcct gctgttcatg tacaaggtca ggaacctttg actgcttcca tgttggcatc 3121 tgcccctcct caagagcaaa agcaaatgtt gggtgaacgg ctgtttcctc ttattcaagc 3181 catgcaccct actcttgctg gtaaaatcac tggcatgttg ttggagattg ataattcaga 3241 acttcttcat atgctcgagt ctccagagtc actccgttct aaggttgatg aagctgtagc 3301 tgtactacaa gcccaccaag ctaaagaggc tgcccagaaa gcagttaaca gtgccaccgg 3361 tgttccaact gttgatatcg actacaaaga ccatgacggt gattataaag atcatgacat 3421 cgactacaag gatgacgatg acaagtaggc ggccgcgact ctagatcata atcagccata 3481 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 3541 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 3601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 3661 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg 3721 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 3781 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 3841 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 3901 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 3961 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 4021 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 4081 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 4141 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 4201 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 4261 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc 4321 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4381 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 4441 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 4501 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 4561 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 4621 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg 4681 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 4741 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 4801 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa 4861 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 4921 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 4981 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 5041 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 5101 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 5161 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc 5221 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 5281 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 5341 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 5401 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 5461 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 5521 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 5581 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 5641 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta 5701 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg 5761 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat 5821 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc 5881 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt 5941 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 6001 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 6061 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 6121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 6181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 6241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 6301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 6361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 6421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 6481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 6541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 6601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 6661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 6721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc 6781 at //