LOCUS       V036457                 5916 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036457
VERSION     V036457
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..1840
                     /label="OPRM1(NM_000914)"
                     /note="OPRM1(NM_000914)"
                     /gene="OPRM1"
     CDS             1862..2581
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2704..2825
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2832..3287)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3314..3418
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3420..3777
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3812..4606
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4838..4885
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5214..5802
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggacagca gcgctgcccc
      661 cacgaacgcc agcaattgca ctgatgcctt ggcgtactca agttgctccc cagcacccag
      721 ccccggttcc tgggtcaact tgtcccactt agatggcaac ctgtccgacc catgcggtcc
      781 gaaccgcacc gacctgggcg ggagagacag cctgtgccct ccgaccggca gtccctccat
      841 gatcacggcc atcacgatca tggccctcta ctccatcgtg tgcgtggtgg ggctcttcgg
      901 aaacttcctg gtcatgtatg tgattgtcag atacaccaag atgaagactg ccaccaacat
      961 ctacattttc aaccttgctc tggcagatgc cttagccacc agtaccctgc ccttccagag
     1021 tgtgaattac ctaatgggaa catggccatt tggaaccatc ctttgcaaga tagtgatctc
     1081 catagattac tataacatgt tcaccagcat attcaccctc tgcaccatga gtgttgatcg
     1141 atacattgca gtctgccacc ctgtcaaggc cttagatttc cgtactcccc gaaatgccaa
     1201 aattatcaat gtctgcaact ggatcctctc ttcagccatt ggtcttcctg taatgttcat
     1261 ggctacaaca aaatacaggc aaggttccat agattgtaca ctaacattct ctcatccaac
     1321 ctggtactgg gaaaacctgc tgaagatctg tgttttcatc ttcgccttca ttatgccagt
     1381 gctcatcatt accgtgtgct atggactgat gatcttgcgc ctcaagagtg tccgcatgct
     1441 ctctggctcc aaagaaaagg acaggaatct tcgaaggatc accaggatgg tgctggtggt
     1501 ggtggctgtg ttcatcgtct gctggactcc cattcacatt tacgtcatca ttaaagcctt
     1561 ggttacaatc ccagaaacta cgttccagac tgtttcttgg cacttctgca ttgctctagg
     1621 ttacacaaac agctgcctca acccagtcct ttatgcattt ctggatgaaa acttcaaacg
     1681 atgcttcaga gagttctgta tcccaacctc ttccaacatt gagcaacaaa actccactcg
     1741 aattcgtcag aacactagag accacccctc cacggccaat acagtggata gaactaatca
     1801 tcagctagaa aatctggaag cagaaactgc tccgttgccc aaggatccac cggtcgccac
     1861 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1921 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1981 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2041 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2101 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2161 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2221 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2281 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2341 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2401 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2461 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2521 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2581 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2641 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2701 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2761 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2821 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2881 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2941 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     3001 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     3061 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3121 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3181 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3241 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3301 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     3421 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3601 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     3781 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     3841 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3901 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     3961 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     4021 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4081 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4141 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4201 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4261 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4321 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4381 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4441 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4501 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4561 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     4621 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     4681 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4741 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     4801 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     4861 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     4921 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     4981 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     5041 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     5101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5881 cctgattctg tggataaccg tattaccgcc atgcat
//
