LOCUS V036457 5916 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036457 VERSION V036457 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..1840 /label="OPRM1(NM_000914)" /note="OPRM1(NM_000914)" /gene="OPRM1" CDS 1862..2581 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2704..2825 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2832..3287) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3314..3418 /gene="bla" /label="AmpR promoter" promoter 3420..3777 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3812..4606 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4838..4885 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5214..5802 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atggacagca gcgctgcccc 661 cacgaacgcc agcaattgca ctgatgcctt ggcgtactca agttgctccc cagcacccag 721 ccccggttcc tgggtcaact tgtcccactt agatggcaac ctgtccgacc catgcggtcc 781 gaaccgcacc gacctgggcg ggagagacag cctgtgccct ccgaccggca gtccctccat 841 gatcacggcc atcacgatca tggccctcta ctccatcgtg tgcgtggtgg ggctcttcgg 901 aaacttcctg gtcatgtatg tgattgtcag atacaccaag atgaagactg ccaccaacat 961 ctacattttc aaccttgctc tggcagatgc cttagccacc agtaccctgc ccttccagag 1021 tgtgaattac ctaatgggaa catggccatt tggaaccatc ctttgcaaga tagtgatctc 1081 catagattac tataacatgt tcaccagcat attcaccctc tgcaccatga gtgttgatcg 1141 atacattgca gtctgccacc ctgtcaaggc cttagatttc cgtactcccc gaaatgccaa 1201 aattatcaat gtctgcaact ggatcctctc ttcagccatt ggtcttcctg taatgttcat 1261 ggctacaaca aaatacaggc aaggttccat agattgtaca ctaacattct ctcatccaac 1321 ctggtactgg gaaaacctgc tgaagatctg tgttttcatc ttcgccttca ttatgccagt 1381 gctcatcatt accgtgtgct atggactgat gatcttgcgc ctcaagagtg tccgcatgct 1441 ctctggctcc aaagaaaagg acaggaatct tcgaaggatc accaggatgg tgctggtggt 1501 ggtggctgtg ttcatcgtct gctggactcc cattcacatt tacgtcatca ttaaagcctt 1561 ggttacaatc ccagaaacta cgttccagac tgtttcttgg cacttctgca ttgctctagg 1621 ttacacaaac agctgcctca acccagtcct ttatgcattt ctggatgaaa acttcaaacg 1681 atgcttcaga gagttctgta tcccaacctc ttccaacatt gagcaacaaa actccactcg 1741 aattcgtcag aacactagag accacccctc cacggccaat acagtggata gaactaatca 1801 tcagctagaa aatctggaag cagaaactgc tccgttgccc aaggatccac cggtcgccac 1861 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 1921 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 1981 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 2041 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 2101 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 2161 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 2221 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 2281 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 2341 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 2401 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 2461 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 2521 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 2581 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 2641 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 2701 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 2761 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 2821 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 2881 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 2941 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 3001 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 3061 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 3121 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 3181 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 3241 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 3301 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 3361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 3421 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3601 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 3721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 3781 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 3841 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 3901 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 3961 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 4021 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 4081 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 4141 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 4201 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 4261 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 4321 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 4381 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4441 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4501 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 4561 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 4621 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 4681 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 4741 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 4801 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 4861 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 4921 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 4981 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 5041 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 5101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 5401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 5881 cctgattctg tggataaccg tattaccgcc atgcat //