LOCUS       V027591                 8809 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027591
VERSION     V027591
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..4717
                     /label="NPC1L1(NM_013389)"
                     /note="NPC1L1(NM_013389)"
                     /gene="NPC1L1"
     CDS             4766..5476
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    5597..5718
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5725..6180)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6207..6311
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6313..6670
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6705..7499
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7731..7778
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8107..8695
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggcggagg ccggcctgag
      661 gggctggctg ctgtgggccc tgctcctgcg cttggcccag agtgagcctt acacaaccat
      721 ccaccagcct ggctactgcg ccttctatga cgaatgtggg aagaacccag agctgtctgg
      781 aagcctcatg acactctcca acgtgtcctg cctgtccaac acgccggccc gcaagatcac
      841 aggtgatcac ctgatcctat tacagaagat ctgcccccgc ctctacaccg gccccaacac
      901 ccaagcctgc tgctccgcca agcagctggt atcactggaa gcgagtctgt cgatcaccaa
      961 ggccctcctc acccgctgcc cagcctgctc tgacaatttt gtgaacctgc actgccacaa
     1021 cacgtgcagc cccaatcaga gcctcttcat caatgtgacc cgcgtggccc agctaggggc
     1081 tggacaactc ccagctgtgg tggcctatga ggccttctac cagcatagct ttgccgagca
     1141 gagctatgac tcctgcagcc gtgtgcgcgt ccctgcagct gccacgctgg ctgtgggcac
     1201 catgtgtggc gtgtatggct ctgccctttg caatgcccag cgctggctca acttccaggg
     1261 agacacaggc aatggtctgg ccccactgga catcaccttc cacctcttgg agcctggcca
     1321 ggccgtgggg agtgggattc agcctctgaa tgagggggtt gcacgttgca atgagtccca
     1381 aggtgacgac gtggcgacct gctcctgcca agactgtgct gcatcctgtc ctgccatagc
     1441 ccgcccccag gccctcgact ccaccttcta cctgggccag atgccgggca gtctggtcct
     1501 catcatcatc ctctgctctg tcttcgctgt ggtcaccatc ctgcttgtgg gattccgtgt
     1561 ggcccccgcc agggacaaaa gcaagatggt ggaccccaag aagggcacca gcctctctga
     1621 caagctcagc ttctccaccc acaccctcct tggccagttc ttccagggct ggggcacgtg
     1681 ggtggcttcg tggcctctga ccatcttggt gctatctgtc atcccggtgg tggccttggc
     1741 agcgggcctg gtctttacag aactcactac ggaccccgtg gagctgtggt cggcccccaa
     1801 cagccaagcc cggagtgaga aagctttcca tgaccagcat ttcggcccct tcttccgaac
     1861 caaccaggtg atcctgacgg ctcctaaccg gtccagctac aggtatgact ctctgctgct
     1921 ggggcccaag aacttcagcg gaatcctgga cctggacttg ctgctggagc tgctagagct
     1981 gcaggagagg ctgcggcacc tccaggtatg gtcgcccgaa gcacagcgca acatctccct
     2041 gcaggacatc tgctacgccc ccctcaatcc ggacaatacc agtctctacg actgctgcat
     2101 caacagcctc ctgcagtatt tccagaacaa ccgcacgctc ctgctgctca cagccaacca
     2161 gacactgatg gggcagacct cccaagtcga ctggaaggac cattttctgt actgtgccaa
     2221 tgccccgctc accttcaagg atggcacagc cctggccctg agctgcatgg ctgactacgg
     2281 ggcccctgtc ttccccttcc ttgccattgg ggggtacaaa ggaaaggact attctgaggc
     2341 agaggccctg atcatgacgt tctccctcaa caattaccct gccggggacc cccgtctggc
     2401 ccaggccaag ctgtgggagg aggccttctt agaggaaatg cgagccttcc agcgtcggat
     2461 ggctggcatg ttccaggtca cgttcatggc tgagcgctct ctggaagacg agatcaatcg
     2521 caccacagct gaagacctgc ccatctttgc caccagctac attgtcatat tcctgtacat
     2581 ctctctggcc ctgggcagct attccagctg gagccgagtg atggtggact ccaaggccac
     2641 gctgggcctc ggcggggtgg ccgtggtcct gggagcagtc atggctgcca tgggcttctt
     2701 ctcctacttg ggtatccgct cctccctggt catcctgcaa gtggttcctt tcctggtgct
     2761 gtccgtgggg gctgataaca tcttcatctt tgttctcgag taccagaggc tgccccggag
     2821 gcctggggag ccacgagagg tccacattgg gcgagcccta ggcagggtgg ctcccagcat
     2881 gctgttgtgc agcctctctg aggccatctg cttcttccta ggggccctga cccccatgcc
     2941 agctgtgcgg acctttgccc tgacctctgg ccttgcagtg atccttgact tcctcctgca
     3001 gatgtcagcc tttgtggccc tgctctccct ggacagcaag aggcaggagg cctcccggtt
     3061 ggacgtctgc tgctgtgtca agccccagga gctgcccccg cctggccagg gagaggggct
     3121 cctgcttggc ttcttccaaa aggcttatgc ccccttcctg ctgcactgga tcactcgagg
     3181 tgttgtgctg ctgctgtttc tcgccctgtt cggagtgagc ctctactcca tgtgccacat
     3241 cagcgtggga ctggaccagg agctggccct gcccaaggac tcgtacctgc ttgactattt
     3301 cctctttctg aaccgctact tcgaggtggg ggccccggtg tactttgtta ccaccttggg
     3361 ctacaacttc tccagcgagg ctgggatgaa tgccatctgc tccagtgcag gctgcaacaa
     3421 cttctccttc acccagaaga tccagtatgc cacagagttc cctgagcagt cttacctggc
     3481 catccctgcc tcctcctggg tggatgactt cattgactgg ctgaccccgt cctcctgctg
     3541 ccgcctttat atatctggcc ccaataagga caagttctgc ccctcgaccg tcaactctct
     3601 gaactgccta aagaactgca tgagcatcac gatgggctct gtgaggccct cggtggagca
     3661 gttccataag tatcttccct ggttcctgaa cgaccggccc aacatcaaat gtcccaaagg
     3721 cggcctggca gcatacagca cctctgtgaa cttgacttca gatggccagg ttttagacac
     3781 agttgccatt ctgtcaccca ggctggagta cagtggcaca atctcggctc actgcaacct
     3841 ctacctcctg gattcaacct ccaggttcat ggcctatcac aagcccctga aaaactcaca
     3901 ggattacaca gaagctctgc gggcagctcg agagctggca gccaacatca ctgctgacct
     3961 gcggaaagtg cctggaacag acccggcttt tgaggtcttc ccctacacga tcaccaatgt
     4021 gttttatgag cagtacctga ccatcctccc tgaggggctc ttcatgctca gcctctgcct
     4081 tgtgcccacc ttcgctgtct cctgcctcct gctgggcctg gacctgcgct ccggcctcct
     4141 caacctgctc tccattgtca tgatcctcgt ggacactgtc ggcttcatgg ccctgtgggg
     4201 catcagttac aatgctgtgt ccctcatcaa cctggtctcg gcggtgggca tgtctgtgga
     4261 gtttgtgtcc cacattaccc gctcctttgc catcagcacc aagcccacct ggctggagag
     4321 ggccaaagag gccaccatct ctatgggaag tgcggtgttt gcaggtgtgg ccatgaccaa
     4381 cctgcctggc atccttgtcc tgggcctcgc caaggcccag ctcattcaga tcttcttctt
     4441 ccgcctcaac ctcctgatca ctctgctggg cctgctgcat ggcttggtct tcctgcccgt
     4501 catcctcagc tacgtggggc ctgacgttaa cccggctctg gcactggagc agaagcgggc
     4561 tgaggaggcg gtggcagcag tcatggtggc ctcttgccca aatcacccct cccgagtctc
     4621 cacagctgac aacatctatg tcaaccacag ctttgaaggt tctatcaaag gtgctggtgc
     4681 catcagcaac ttcttgccca acaatgggcg gcagttcggt ggaggcggat caggaggcgg
     4741 tggatcggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat
     4801 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     4861 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4921 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     4981 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     5041 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     5101 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     5161 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     5221 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     5281 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     5341 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     5401 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     5461 cgagctgtac aagtagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     5521 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5581 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5641 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5701 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     5761 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5821 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5881 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5941 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6001 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6061 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6121 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6181 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6241 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6301 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     6361 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6421 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6481 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6541 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6601 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6661 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     6721 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     6781 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     6841 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     6901 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6961 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7021 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7081 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7141 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7201 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     7261 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7321 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7381 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7441 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     7501 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     7561 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7621 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     7681 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     7741 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     7801 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     7861 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     7921 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     7981 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8041 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8101 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8161 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8221 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8281 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8341 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8401 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8461 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8521 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8581 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8641 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8701 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8761 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
