LOCUS       V020600                 4936 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020600
VERSION     V020600
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             634..1584
                     /label="NFKBIA(NM_020529)"
                     /note="NFKBIA(NM_020529)"
                     /gene="NFKBIA"
     polyA_signal    1724..1845
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(1852..2307)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        2334..2438
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        2440..2797
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             2832..3626
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    3858..3905
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4234..4822
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttgcc accatgttcc aggcggccga gcgcccccag
      661 gagtgggcca tggagggccc ccgcgacggg ctgaagaagg agcggctact ggacgaccgc
      721 cacgacagcg gcctggactc catgaaagac gaggagtacg agcagatggt caaggagctg
      781 caggagatcc gcctcgagcc gcaggaggtg ccgcgcggct cggagccctg gaagcagcag
      841 ctcaccgagg acggggactc gttcctgcac ttggccatca tccatgaaga aaaggcactg
      901 accatggaag tgatccgcca ggtgaaggga gacctggcct tcctcaactt ccagaacaac
      961 ctgcagcaga ctccactcca cttggctgtg atcaccaacc agccagaaat tgctgaggca
     1021 cttctgggag ctggctgtga tcctgagctc cgagactttc gaggaaatac ccccctacac
     1081 cttgcctgtg agcagggctg cctggccagc gtgggagtcc tgactcagtc ctgcaccacc
     1141 ccgcacctcc actccatcct gaaggctacc aactacaatg gccacacgtg tctacactta
     1201 gcctctatcc atggctacct gggcatcgtg gagcttttgg tgtccttggg tgctgatgtc
     1261 aatgctcagg agccctgtaa tggccggact gcccttcacc tcgcagtgga cctgcaaaat
     1321 cctgacctgg tgtcactcct gttgaagtgt ggggctgatg tcaacagagt tacctaccag
     1381 ggctattctc cctaccagct cacctggggc cgcccaagca cccggataca gcagcagctg
     1441 ggccagctga cactagaaaa ccttcagatg ctgccagaga gtgaggatga ggagagctat
     1501 gacacagagt cagagttcac ggagttcaca gaggacgagc tgccctatga tgactgtgtg
     1561 tttggaggcc agcgtctgac gttatgagga tccatcgcca ccgcggccgc gactctagat
     1621 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     1681 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     1741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     1861 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1921 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1981 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2041 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2101 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2161 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2221 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2281 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2401 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     2461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     2581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     2641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     2701 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     2761 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     2821 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     2881 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     2941 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3001 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3061 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3121 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3181 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3241 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3301 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     3361 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3421 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3481 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3541 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3601 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     3661 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     3721 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     3781 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     3841 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     3901 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     3961 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     4021 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     4081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4381 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4921 tattaccgcc atgcat
//
