LOCUS       V026696                 6561 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026696
VERSION     V026696
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2455
                     /label="NFE2L2(NM_006164)"
                     /note="NFE2L2(NM_006164)"
                     /gene="NFE2L2"
     CDS             2507..3226
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3349..3470
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3477..3932)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3959..4063
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4065..4422
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4457..5251
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5483..5530
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5859..6447
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgatggact tggagctgcc
      661 gccgccggga ctcccgtccc agcaggacat ggatttgatt gacatacttt ggaggcaaga
      721 tatagatctt ggagtaagtc gagaagtatt tgacttcagt cagcgacgga aagagtatga
      781 gctggaaaaa cagaaaaaac ttgaaaagga aagacaagaa caactccaaa aggagcaaga
      841 gaaagccttt ttcgctcagt tacaactaga tgaagagaca ggtgaatttc tcccaattca
      901 gccagcccag cacatccagt cagaaaccag tggatctgcc aactactccc aggttgccca
      961 cattcccaaa tcagatgctt tgtactttga tgactgcatg cagcttttgg cgcagacatt
     1021 cccgtttgta gatgacaatg aggtttcttc ggctacgttt cagtcacttg ttcctgatat
     1081 tcccggtcac atcgagagcc cagtcttcat tgctactaat caggctcagt cacctgaaac
     1141 ttctgttgct caggtagccc ctgttgattt agacggtatg caacaggaca ttgagcaagt
     1201 ttgggaggag ctattatcca ttcctgagtt acagtgtctt aatattgaaa atgacaagct
     1261 ggttgagact accatggttc caagtccaga agccaaactg acagaagttg acaattatca
     1321 tttttactca tctataccct caatggaaaa agaagtaggt aactgtagtc cacattttct
     1381 taatgctttt gaggattcct tcagcagcat cctctccaca gaagacccca accagttgac
     1441 agtgaactca ttaaattcag atgccacagt caacacagat tttggtgatg aattttattc
     1501 tgctttcata gctgagccca gtatcagcaa cagcatgccc tcacctgcta ctttaagcca
     1561 ttcactctct gaacttctaa atgggcccat tgatgtttct gatctatcac tttgcaaagc
     1621 tttcaaccaa aaccaccctg aaagcacagc agaattcaat gattctgact ccggcatttc
     1681 actaaacaca agtcccagtg tggcatcacc agaacactca gtggaatctt ccagctatgg
     1741 agacacacta cttggcctca gtgattctga agtggaagag ctagatagtg cccctggaag
     1801 tgtcaaacag aatggtccta aaacaccagt acattcttct ggggatatgg tacaaccctt
     1861 gtcaccatct caggggcaga gcactcacgt gcatgatgcc caatgtgaga acacaccaga
     1921 gaaagaattg cctgtaagtc ctggtcatcg gaaaacccca ttcacaaaag acaaacattc
     1981 aagccgcttg gaggctcatc tcacaagaga tgaacttagg gcaaaagctc tccatatccc
     2041 attccctgta gaaaaaatca ttaacctccc tgttgttgac ttcaacgaaa tgatgtccaa
     2101 agagcagttc aatgaagctc aacttgcatt aattcgggat atacgtagga ggggtaagaa
     2161 taaagtggct gctcagaatt gcagaaaaag aaaactggaa aatatagtag aactagagca
     2221 agatttagat catttgaaag atgaaaaaga aaaattgctc aaagaaaaag gagaaaatga
     2281 caaaagcctt cacctactga aaaaacaact cagcacctta tatctcgaag ttttcagcat
     2341 gctacgtgat gaagatggaa aaccttattc tcctagtgaa tactccctgc agcaaacaag
     2401 agatggcaat gttttccttg ttcccaaaag taagaagcca gatgttaaga aaaacggtgg
     2461 aggcggatca ggaggcggtg gatctgggga tccaccggtc gccaccatgg tgagcaaggg
     2521 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2581 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2641 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2701 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2761 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2821 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2881 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2941 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3001 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3061 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3121 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3181 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     3241 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3301 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3361 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3421 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     3481 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3541 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3601 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3661 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3721 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3781 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3841 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3901 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     4081 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4141 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4201 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4261 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4321 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4381 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4441 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4501 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4561 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4621 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4681 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4741 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4801 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4861 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4921 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4981 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5041 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5101 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5161 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5221 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5281 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5341 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5401 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5461 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5521 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5581 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5641 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5701 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5761 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5821 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5881 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5941 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6001 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6061 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6121 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6181 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6241 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6301 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6361 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6421 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6481 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6541 aaccgtatta ccgccatgca t
//
