LOCUS V027366 5595 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027366 VERSION V027366 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..1492 /label="Icmt(NM_133310)" /note="Icmt(NM_133310)" /gene="Icmt" CDS 1541..2260 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2383..2504 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2511..2966) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2993..3097 /gene="bla" /label="AmpR promoter" promoter 3099..3456 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3491..4285 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4517..4564 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4893..5481 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atggcgggct gcgcggcgcg 661 ggttccgccg ggctccgagg cgcgcctcag cctcgctacc ttcctccttg gtgcctcggt 721 gctcgctctg cctctgctca cgcgcgccgg cctgcagggc cgcaccgggc tggcgctgta 781 cgtggccgga ctcaacgcgc tgttgctgct tctctaccgg ccgccgcgct accagatagc 841 catccgagct tgtttccttg gctttgtgtt tggctgcggc gtgctgctaa gtttcagcca 901 gtcctcctgg aatcacttcg gctggtacgt gtgctcgctc tccctgttcc actactctga 961 gtacctagtg acagctgtca ataatcccaa aagtctgtcc ttggactcct tcctcctgaa 1021 ccacagtctg gagtacacag tggctgccct gtcttcctgg atagagttca cccttgaaaa 1081 catcttttgg ccagaactga agcagattac ctggctcagt gccgctgggc tgttgatggt 1141 gatctttgga gaatgcctga ggaaagtggc catgtttaca gccggctcca acttcaacca 1201 cgtggtacag agtgaaaagt cagacaccca cactctcgtg acaagtgggg tatacgcttg 1261 gtgccggcac ccctcctatg tgggctggtt ttactggagt attggaaccc aggtgatgct 1321 gtgtaatccc atctgtggtg tggtctatgc cctgacggtg tggcgcttct tccgtgatcg 1381 cacagaggag gaggagatct cgctgattca cttctttggg gaagaatact tggactataa 1441 gaagagggtg cccacaggcc tgcctttcat aaagggggtc aaggtggggc taggtggagg 1501 cggatcagga ggcggtggat cggatccacc ggtcgccacc atggtgagca agggcgagga 1561 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 1621 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 1681 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 1741 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 1801 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 1861 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 1921 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 1981 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 2041 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 2101 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 2161 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 2221 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc 2281 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 2341 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 2401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 2461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag 2521 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca 2581 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 2641 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 2701 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 2761 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 2821 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 2881 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 2941 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac 3001 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 3061 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg 3121 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3181 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3241 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3301 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3361 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 3421 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg 3481 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 3541 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 3601 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 3661 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 3721 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 3781 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 3841 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 3901 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 3961 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag 4021 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat 4081 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 4141 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 4201 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 4261 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 4321 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc 4381 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 4441 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa 4501 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt 4561 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc 4621 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt 4681 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac 4741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 4801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 4861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 4921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 5041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 5101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 5161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 5221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 5281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 5341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 5401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 5461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 5521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 5581 attaccgcca tgcat //