LOCUS       V028091                 9148 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028091
VERSION     V028091
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..5032
                     /label="GRIN2A(NM_001134407)"
                     /note="GRIN2A(NM_001134407)"
                     /gene="GRIN2A"
     CDS             5039..5065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5069..5095
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5099..5125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5138..5815
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /transl_table=1
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL*"
                     /label="DsRed2"
     polyA_signal    5936..6057
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(6064..6519)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6546..6650
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6652..7009
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7044..7838
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8070..8117
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8446..9034
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgggcagag tgggctattg
      661 gaccctgctg gtgctgccgg cccttctggt ctggcgcggt ccggcgccga gcgcggcggc
      721 ggagaagggt ccccccgcgc taaatattgc ggtgatgctg ggtcacagcc acgacgtgac
      781 agagcgcgaa cttcgaacac tgtggggccc cgagcaggcg gcggggctgc ccctggacgt
      841 gaacgtggta gctctgctga tgaaccgcac cgaccccaag agcctcatca cgcacgtgtg
      901 cgacctcatg tccggggcac gcatccacgg cctcgtgttt ggggacgaca cggaccagga
      961 ggccgtagcc cagatgctgg attttatctc ctcccacacc ttcgtcccca tcttgggcat
     1021 tcatgggggc gcatctatga tcatggctga caaggatccg acgtctacct tcttccagtt
     1081 tggagcgtcc atccagcagc aagccacggt catgctgaag atcatgcagg attatgactg
     1141 gcatgtcttc tccctggtga ccactatctt ccctggctac agggaattca tcagcttcgt
     1201 caagaccaca gtggacaaca gctttgtggg ctgggacatg cagaatgtga tcacactgga
     1261 cacttccttt gaggatgcaa agacacaagt ccagctgaag aagatccact cttctgtcat
     1321 cttgctctac tgttccaaag acgaggctgt tctcattctg agtgaggccc gctcccttgg
     1381 cctcaccggg tatgatttct tctggattgt ccccagcttg gtctctggga acacggagct
     1441 catcccaaaa gagtttccat cgggactcat ttctgtctcc tacgatgact gggactacag
     1501 cctggaggcg agagtgaggg acggcattgg catcctaacc accgctgcat cttctatgct
     1561 ggagaagttc tcctacatcc ccgaggccaa ggccagctgc tacgggcaga tggagaggcc
     1621 agaggtcccg atgcacacct tgcacccatt tatggtcaat gttacatggg atggcaaaga
     1681 cttatccttc actgaggaag gctaccaggt gcaccccagg ctggtggtga ttgtgctgaa
     1741 caaagaccgg gaatgggaaa aggtgggcaa gtgggagaac catacgctga gcctgaggca
     1801 cgccgtgtgg cccaggtaca agtccttctc cgactgtgag ccggatgaca accatctcag
     1861 catcgtcacc ctggaggagg ccccattcgt catcgtggaa gacatagacc ccctgaccga
     1921 gacgtgtgtg aggaacaccg tgccatgtcg gaagttcgtc aaaatcaaca attcaaccaa
     1981 tgaggggatg aatgtgaaga aatgctgcaa ggggttctgc attgatattc tgaagaagct
     2041 ttccagaact gtgaagttta cttacgacct ctatctggtg accaatggga agcatggcaa
     2101 gaaagttaac aatgtgtgga atggaatgat cggtgaagtg gtctatcaac gggcagtcat
     2161 ggcagttggc tcgctcacca tcaatgagga acgttctgaa gtggtggact tctctgtgcc
     2221 ctttgtggaa acgggaatca gtgtcatggt ttcaagaagt aatggcaccg tctcaccttc
     2281 tgcttttcta gaaccattca gcgcctctgt ctgggtgatg atgtttgtga tgctgctcat
     2341 tgtttctgcc atagctgttt ttgtctttga atacttcagc cctgttggat acaacagaaa
     2401 cttagccaaa gggaaagcac cccatgggcc ttcttttaca attggaaaag ctatatggct
     2461 tctttggggc ctggtgttca ataactccgt gcctgtccag aatcctaaag ggaccaccag
     2521 caagatcatg gtatctgtat gggccttctt cgctgtcata ttcctggcta gctacacagc
     2581 caatctggct gccttcatga tccaagagga atttgtggac caagtgaccg gcctcagtga
     2641 caaaaagttt cagagacctc atgactattc cccacctttt cgatttggga cagtgcctaa
     2701 tggaagcacg gagagaaaca ttcggaataa ctatccctac atgcatcagt acatgaccaa
     2761 atttaatcag aaaggagtag aggacgcctt ggtcagcctg aaaacgggga agctggacgc
     2821 tttcatctac gatgccgcag tcttgaatta caaggctggg agggatgaag gctgcaagct
     2881 ggtgaccatc gggagtgggt acatctttgc caccaccggt tatggaattg cccttcagaa
     2941 aggctctcct tggaagaggc agatcgacct ggccttgctt cagtttgtgg gtgatggtga
     3001 gatggaggag ctggagaccc tgtggctcac tgggatctgc cacaacgaga agaacgaggt
     3061 gatgagcagc cagctggaca ttgacaacat ggcgggcgta ttctacatgc tggctgccgc
     3121 catggccctt agcctcatca ccttcatctg ggagcacctc ttctactgga agctgcgctt
     3181 ctgtttcacg ggcgtgtgct ccgaccggcc tgggttgctc ttctccatca gcaggggcat
     3241 ctacagctgc attcatggag tgcacattga agaaaagaag aagtctccag acttcaatct
     3301 gacgggatcc cagagcaaca tgttaaaact cctccggtca gccaaaaaca tttccagcat
     3361 gtccaacatg aactcctcaa gaatggactc acccaaaaga gctgctgact tcatccaaag
     3421 aggttccctc atcatggaca tggtttcaga taaggggaat ttgatgtact cagacaacag
     3481 gtcctttcag gggaaagaga gcatttttgg agacaacatg aacgaactcc aaacatttgt
     3541 ggccaaccgg cagaaggata acctcaataa ctatgtattc cagggacaac atcctcttac
     3601 tctcaatgag tccaacccta acacggtgga ggtggccgtg agcacagaat ccaaagcgaa
     3661 ctctagaccc cggcagctgt ggaagaaatc cgtggattcc atacgccagg attcactatc
     3721 ccagaatcca gtctcccaga gggatgaggc aacagcagag aataggaccc actccctaaa
     3781 gagccctagg tatcttccag aagagatggc ccactctgac atttcagaaa cgtcaaatcg
     3841 ggccacgtgc cacagggaac ctgacaacag taagaaccac aaaaccaagg acaactttaa
     3901 aaggtcagtg gcctccaaat accccaagga ctgtagtgag gtcgagcgca cctacctgaa
     3961 aaccaaatca agctccccta gagacaagat ctacactata gatggtgaga aggagcctgg
     4021 tttccactta gatccacccc agtttgttga aaatgtgacc ctgcccgaga acgtggactt
     4081 cccggacccc taccaggatc ccagtgaaaa cttccgcaag ggggactcca cgctgccaat
     4141 gaaccggaac cccttgcata atgaagaggg gctttccaac aacgaccagt ataaactcta
     4201 ctccaagcac ttcaccttga aagacaaggg ttccccgcac agtgagacca gcgagcgata
     4261 ccggcagaac tccacgcact gcagaagctg cctttccaac atgcccacct attcaggcca
     4321 cttcaccatg aggtccccct tcaagtgcga tgcctgcctg cggatgggga acctctatga
     4381 catcgatgaa gaccagatgc ttcaggagac aggtaaccca gccaccgggg agcaggtcta
     4441 ccagcaggac tgggcacaga acaatgccct tcaattacaa aagaacaagc taaggattag
     4501 ccgtcagcat tcctacgata acattgtcga caaacctagg gagctagacc ttagcaggcc
     4561 ctcccggagc ataagcctca aggacaggga acggcttctg gagggaaatt tttacggcag
     4621 cctgtttagt gtcccctcaa gcaaactctc ggggaaaaaa agctcccttt tcccccaagg
     4681 tctggaggac agcaagagga gcaagtctct cttgccagac cacacctccg ataacccttt
     4741 cctccactcc cacagggatg accaacgctt ggttattggg agatgcccct cggaccctta
     4801 caaacactcg ttgccatccc aggcggtgaa tgacagctat cttcggtcgt ccttgaggtc
     4861 aacggcatcg tactgttcca gggacagtcg gggccacaat gatgtgtata tttcggagca
     4921 tgttatgcct tatgctgcaa ataagaataa tatgtactct acccccaggg ttttaaattc
     4981 ctgcagcaat agacgcgtgt acaagaaaat gcctagtatc gaatctgatg ttggatccta
     5041 cccatacgac gtacctgatt atgcaactta tccttatgat gtacctgatt atgctagcta
     5101 tccttatgat gtcccagact acgcaccggt cgccaccatg gcctcctccg agaacgtcat
     5161 caccgagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga
     5221 gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt
     5281 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg
     5341 ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc
     5401 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac
     5461 ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa
     5521 cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctccaccga
     5581 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa
     5641 ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca
     5701 gctgcccggc tactactacg tggacgccaa gctggacatc acctcccaca acgaggacta
     5761 caccatcgtg gagcagtacg agcgcaccga gggccgccac cacctgttcc tgtagcggcc
     5821 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5881 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5941 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6001 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     6061 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6121 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6181 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6241 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6301 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6361 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6421 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     6481 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     6541 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6601 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     6661 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6841 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6901 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6961 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     7021 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     7081 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     7141 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     7201 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     7261 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     7321 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7381 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     7441 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     7501 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     7561 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     7621 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     7681 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     7741 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     7801 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     7861 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     7921 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7981 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     8041 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     8101 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     8161 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     8221 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     8281 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8341 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8401 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8461 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8521 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8581 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8641 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8701 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8761 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8821 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8881 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8941 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9001 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9061 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9121 tgtggataac cgtattaccg ccatgcat
//
