LOCUS       V034220                 5860 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034220
VERSION     V034220
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        67..370
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        371..574
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        620..638
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     regulatory      673..682
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             721..2265
                     /label="G6PD(NM_001042351)"
                     /note="G6PD(NM_001042351)"
                     /gene="G6PD"
     primer_bind     complement(2289..2305)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="KS primer"
     promoter        complement(2362..2380)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     polyA_signal    2654..2775
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2782..3237)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3264..3368
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3370..3727
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3762..4556
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4788..4835
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5164..5752
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
      721 atggcagagc aggtggccct gagccggacc caggtgtgcg ggatcctgcg ggaagagctt
      781 ttccagggcg atgccttcca tcagtcggat acacacatat tcatcatcat gggtgcatcg
      841 ggtgacctgg ccaagaagaa gatctacccc accatctggt ggctgttccg ggatggcctt
      901 ctgcccgaaa acaccttcat cgtgggctat gcccgttccc gcctcacagt ggctgacatc
      961 cgcaaacaga gtgagccctt cttcaaggcc accccagagg agaagctcaa gctggaggac
     1021 ttctttgccc gcaactccta tgtggctggc cagtacgatg atgcagcctc ctaccagcgc
     1081 ctcaacagcc acatgaatgc cctccacctg gggtcacagg ccaaccgcct cttctacctg
     1141 gccttgcccc cgaccgtcta cgaggccgtc accaagaaca ttcacgagtc ctgcatgagc
     1201 cagataggct ggaaccgcat catcgtggag aagcccttcg ggagggacct gcagagctct
     1261 gaccggctgt ccaaccacat ctcctccctg ttccgtgagg accagatcta ccgcatcgac
     1321 cactacctgg gcaaggagat ggtgcagaac ctcatggtgc tgagatttgc caacaggatc
     1381 ttcggcccca tctggaaccg ggacaacatc gcctgcgtta tcctcacctt caaggagccc
     1441 tttggcactg agggtcgcgg gggctatttc gatgaatttg ggatcatccg ggacgtgatg
     1501 cagaaccacc tactgcagat gctgtgtctg gtggccatgg agaagcccgc ctccaccaac
     1561 tcagatgacg tccgtgatga gaaggtcaag gtgttgaaat gcatctcaga ggtgcaggcc
     1621 aacaatgtgg tcctgggcca gtacgtgggg aaccccgatg gagagggcga ggccaccaaa
     1681 gggtacctgg acgaccccac ggtgccccgc gggtccacca ccgccacttt tgcagccgtc
     1741 gtcctctatg tggagaatga gaggtgggat ggggtgccct tcatcctgcg ctgcggcaag
     1801 gccctgaacg agcgcaaggc cgaggtgagg ctgcagttcc atgatgtggc cggcgacatc
     1861 ttccaccagc agtgcaagcg caacgagctg gtgatccgcg tgcagcccaa cgaggccgtg
     1921 tacaccaaga tgatgaccaa gaagccgggc atgttcttca accccgagga gtcggagctg
     1981 gacctgacct acggcaacag atacaagaac gtgaagctcc ctgacgccta tgagcgcctc
     2041 atcctggacg tcttctgcgg gagccagatg cacttcgtgc gcagcgacga gctccgtgag
     2101 gcctggcgta ttttcacccc actgctgcac cagattgagc tggagaagcc caagcccatc
     2161 ccctatattt atggcagccg aggccccacg gaggcagacg agctgatgaa gagagtgggt
     2221 ttccagtatg agggcaccta caagtgggtg aacccccaca agctctgaga attcgatatc
     2281 aagcttatcg ataccgtcga cctcgagggg gggcccggta ccttaattaa ttaaggtacc
     2341 aggtaagtgt acccaattcg ccctatagtg agtcgtatta caattcactc gatcgccctt
     2401 cccaacagtt gcgcagcctg aatggcgaat ggagatccaa tttttaagtg tataatgtgt
     2461 taaactactg attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag
     2521 gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt
     2581 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2641 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2701 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2761 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     2821 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     2881 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     2941 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3001 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3061 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3121 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3181 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     3241 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3301 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3361 ggaagaatcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     3421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3481 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3541 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3601 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3661 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3721 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     3781 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     3841 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     3901 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     3961 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4021 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4081 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4141 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4201 ggatggaagc cggtcttgtc gatcaggatg atctggacga agaacatcag gggctcgcgc
     4261 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     4321 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4381 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4441 atattgctga agaacttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4501 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     4561 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     4621 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     4681 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     4741 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     4801 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     4861 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     4921 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     4981 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     5041 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5101 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5161 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5221 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5281 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5341 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5401 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5461 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5521 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5581 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5641 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5701 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5761 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5821 tgcgttatcc cctgattctg tggataaccg tattaccgcc
//
