LOCUS       V018916                 6664 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018916
VERSION     V018916
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2554
                     /label="DYNC1I2(NM_001271785)"
                     /note="DYNC1I2(NM_001271785)"
                     /gene="DYNC1I2"
     CDS             2621..3331
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    3452..3573
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3580..4035)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4062..4166
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4168..4525
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4560..5354
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5586..5633
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5962..6550
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgtcagaca aaagtgaatt
      661 aaaggctgag ttggaacgta agaagcagcg actggcccaa atcagagagg aaaagaagag
      721 aaaagaagaa gaaaggaaaa aaaaagaaac agaccagaag aaggaagctg ttgctcctgt
      781 gcaagaagaa tcagatcttg aaaaaaaaag gagagaagct gaagcattgc ttcaaagcat
      841 ggggctaact ccagaatccc ccattgtttt ttctgaatac tgggtccctc ctcctatgtc
      901 tccatcctcc aaatctgtga gcactccaag tgaagctgga agccaagact ctggagatgg
      961 cgccgtggga tctaggacgc tgcattggga tacagatcca tcagttcttc agcttcactc
     1021 agattccgat ttgggacgag gacctattaa acttggaatg gctaaaatca cgcaagtcga
     1081 ctttcctcct cgagaaattg tcacgtatac aaaggaaact cagactccag ttatggctca
     1141 acccaaagaa gatgaagagg aagatgatga tgtagtggct cctaaaccac ctattgaacc
     1201 tgaagaagag aaaactttaa agaaagatga ggaaaatgat agtaaagctc cccctcatga
     1261 gctgactgaa gaagaaaagc aacaaatctt gcactctgag gaatttttaa gtttctttga
     1321 ccattctaca agaattgtag aaagagctct ttctgagcag attaacatct tctttgacta
     1381 tagtgggaga gatttggaag acaaagaagg agagattcaa gcaggtgcta aactgtcatt
     1441 aaatcgacaa ttttttgacg aacgttggtc aaagcatcgg gtggttagtt gtttggattg
     1501 gtcatctcag tatccggagt tactcgtggc ttcctataac aacaatgaag atgcccctca
     1561 tgagcctgat ggtgtggccc ttgtatggaa tatgaaatac aaaaaaacta ccccagagta
     1621 tgtgtttcac tgccagtcag ctgtgatgtc tgccacattt gcaaaatttc atccaaatct
     1681 tgttgttggt ggtacatatt caggccaaat tgtgctttgg gataaccgta gcaataaaag
     1741 aactccagtg caaagaactc cactgtcagc agctgcacac acacaccctg tatattgtgt
     1801 aaatgttgtt ggaacacaaa atgctcacaa tctgattagc atctctactg atggaaaaat
     1861 ttgttcatgg agtctggaca tgctttccca tccacaggat agcatggagt tggttcataa
     1921 acagtcaaaa gcagtagctg tgacatctat gtccttccct gttggagatg tcaacaactt
     1981 tgttgttggg agtgaagaag gttctgtgta cacagcatgc cgccatggca gcaaagctgg
     2041 aatcagtgag atgtttgagg ggcatcaagg accaatcact ggcatccatt gtcatgcagc
     2101 tgttggagca gtagacttct cacatctttt tgtcacttca tcgtttgact ggacagtaaa
     2161 gctttggaca actaagaata acaagccttt gtattcattt gaagataatg cagactatgt
     2221 ttatgatgtt atgtggtcac ctacccaccc agccctgttt gcctgtgtgg atggcatggg
     2281 gagattggat ttgtggaatc tcaataatga cacagaggta ccaactgcca gcatttctgt
     2341 ggagggtaat cctgctctta atcgtgtgag atggacccat tctggcagag agattgctgt
     2401 gggtgattct gaaggacaga ttgttatata cgatgtggga gagcagattg ctgttccccg
     2461 caatgatgaa tgggcacggt ttggccgaac acttgcagaa attaatgcaa accgagctga
     2521 tgcagaggag gaagcagcta cccgaatacc tgctggcggc ggagggagtg gaggaggggg
     2581 aagcggaggc ggagggagtc aggatccacc ggtcgccacc atggtgagca agggcgagga
     2641 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     2701 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2761 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     2821 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     2881 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2941 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3001 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     3061 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     3121 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     3181 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     3241 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     3301 caccggcggc atggacgagc tgtacaagta gcggccgcga ctctagatca taatcagcca
     3361 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     3421 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     3481 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3541 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     3601 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3661 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3721 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3781 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3841 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3901 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     3961 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4021 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4081 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4141 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     4201 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4261 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4321 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4381 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4441 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4501 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     4561 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4621 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4681 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4741 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4801 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4861 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4921 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4981 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5041 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     5101 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5161 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5221 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5281 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5341 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5401 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5461 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5521 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5581 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     5641 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     5701 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     5761 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     5821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     6661 gcat
//
