LOCUS       V032354                 8605 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032354
VERSION     V032354
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..4504
                     /label="CNTNAP3(NM_033655)"
                     /note="CNTNAP3(NM_033655)"
                     /gene="CNTNAP3"
     CDS             4562..5272
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    5393..5514
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5521..5976)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6003..6107
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6109..6466
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6501..7295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7527..7574
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7903..8491
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggcttcag tggcctgggc
      661 cgtcctcaag gtgctgctgc ttctccccac tcagacttgg agccccgtgg gagcaggaaa
      721 tccacctgac tgtgatgccc cactggcctc tgccttgcct aggtcatcct tcagcagctc
      781 ctcagagctg tccagcagcc acggcccggg gttttcaagg cttaatcgaa gagatggagc
      841 tggtggctgg actccacttg tgtcaaataa ataccaatgg ctgcaaattg accttggaga
      901 gagaatggag gtcactgctg tcgccaccca aggaggatat gggagctctg actgggtgac
      961 cagctacctc ctgatgttca gtgatggtgg gagaaactgg aagcagtatc gccgagaaga
     1021 aagcatctgg ggttttccag gaaacacaaa cgcagacagt gtggtgcact acagactcca
     1081 gcctcccttt gaagccaggt tcctgcgctt tctcccttta gcctggaacc ctaggggcag
     1141 gattgggatg cggatcgaag tgtacggatg tgcatataaa tctgaggtgg tttattttga
     1201 tggacaaagt gctctgctgt atagacttga taaaaaacct ttaaaaccaa taagagacgt
     1261 tatttctttg aaatttaaag ccatgcagag caatggaatt ctacttcaca gagaaggaca
     1321 acatggaaat cacattactc tggaattaat taaaggaaag cttgtctttt ttcttaattc
     1381 aggcaatgct aagctgcctt ccactattgc tcctgtgacc ctcaccctgg gcagcctgct
     1441 ggacgaccag cactggcatt ccgtcctcat cgagctcctc gacacgcagg tcaacttcac
     1501 cgtggacaaa cacactcatc atttccaagc aaagggagat tccagttact tggatcttaa
     1561 ttttgagatc agctttgggg gaattccgac acccggaaga tcgcgggcat tcagacgtaa
     1621 aagctttcat gggtgtttag aaaatcttta ttataatgga gtggatgtta ccgaattagc
     1681 caagaaacac aaaccacaga tcctcatgat gggaaatgtg tccttctcat gtccacagcc
     1741 acagactgtc cctgtgactt ttctgagctc caggagttat ctggctctgc caggcaactc
     1801 tggggaggac aaagtgtctg tcacttttca atttcgaacg tggaacagag caggacattt
     1861 gcttttcggc gaacttcgac gtggttcagg gagtttcgtc ctctttctta aggatggcaa
     1921 gctcaaactg agtctcttcc agccgggaca gtcaccaagg aatgtcacag caggtgctgg
     1981 attaaacgat gggcagtggc actctgtgtc cttctctgcc aagtggagcc atatgaatgt
     2041 ggtggtggac gatgacacag ctgttcagcc cctggtggct gtgctcattg attcaggtga
     2101 cacctattat tttggaggct gcctggacaa cagctctggc tctggatgta aaagccccct
     2161 gggagggttt cagggctgcc taaggctcat caccattggt gacaaagcgg tggatcccat
     2221 cttagtacag cagggggcgc tggggagttt cagggacctc cagatagact cctgcggcat
     2281 cacagacagg tgcttgccca gctactgtga gcatgggggc gagtgttccc agtcgtggga
     2341 caccttctcc tgtgactgtc taggcacagg ctatacgggc gagacctgcc attcctctct
     2401 ctacgagcag tcttgtgaag cccacaagca ccgagggaac ccgtctgggc tttactatat
     2461 tgatgcagat ggaagtggcc ccctgggacc atttcttgtg tactgcaata tgacagcaga
     2521 cgccgcgtgg acggtggtgc agcacggtgg ccccgacgcg gtgaccctcc gaggtgcccc
     2581 cagcgggcac ccgcgctcgg ctgtgtcctt cgcgtacgca gcgggcgcgg ggcagctgcg
     2641 gtccgcggtg aacctggcgg agcgctgcga gcagcggctg gctctgcgct gcgggacggc
     2701 gcggcgcccg gactcacgag atggaacccc actgagctgg tgggttggaa gaaccaatga
     2761 aacacacact tcctggggag gttctctgcc tgatgctcaa aagtgtactt gtggattaga
     2821 ggggaactgc attgattctc agtattactg caactgtgat gctggccgga atgaatggac
     2881 tagtgacaca atagtccttt cccaaaagga gcacctgcca gtcactcaga ttgtgatgac
     2941 agacgcaggc cgaccacatt ccgaagcagc ttatacactg gggccactgc tctgccgcgg
     3001 agatcagtca ttctggaatt cagcttcctt caacactgag acttcatacc ttcatttccc
     3061 tgctttccac ggagaactca ctgctgacgt gtgcttcttt tttaagacca cagtttcctc
     3121 cggggtgttt atggagaacc tggggatcac agatttcatc aggattgagc tgcgtgctcc
     3181 cacagaagtg accttttcct tcgatgtggg gaatggacct tgtgaggtca cggtgcagtc
     3241 acccactccc tttaatgaca atcagtggca ccacgtgagg gcagagagaa atgttaaagg
     3301 agcgtctctt caagttgatc agcttcctca gaagatgcag cctgcccctg ctgatgggca
     3361 cgttcgttta cagctcaaca gccagctctt cattggtgga acggccacca gacagagagg
     3421 ctttctagga tgcattcggt ctctgcagtt gaacggggtg gccctggatc tggaagaaag
     3481 agccacagtg acgccaggag tggagccagg gtgtgcagga cactgcagca cctatggaca
     3541 cttgtgtcgc aatggaggga gatgcagaga gaaacgcagg ggggtcacct gtgactgtgc
     3601 cttctcagcc tatgatgggc cgttctgctc caatgagatt tccgcatatt ttgcaactgg
     3661 ctcctcaatg acataccatt ttcaagaaca ttacacttta agtgaaaact ccagctctct
     3721 cgtttcttca ttacacagag atgtaacatt gaccagagaa atgatcacac tgagcttccg
     3781 aaccacacga actccgagct tattgctgta tgtgagctct ttctatgagg aatacctttc
     3841 agttatcctc gccaacaatg gaagtttgca gattaggtac aagctagata gacatcaaaa
     3901 tcctgatgca tttacctttg attttaaaaa catggctgat gggcaacttc accaagtgaa
     3961 gattaacaga gaagaagctg tggtcatggt agaggttaac cagagcacaa agaaacaagt
     4021 catcttgtcc tcagggacag aattcaacgc cgtcaaatct ctcatattgg gaaaggtttt
     4081 agaggctgcc ggcgcggacc cggacacaag gcgggcggcg actagtggct tcactggctg
     4141 cctctcggcg gtgcgcttcg gccgcgctgc tcccctgaag gcggcgctgc gccccagcgg
     4201 cccctcccgg gtcaccgtcc gcggccacgt ggcccctatg gcccgctgcg cagcgggggc
     4261 ggcgtccggc tccccggcgc gggaactggc tccccgactc gcggggggcg caggtcgttc
     4321 tggaccagcg gatgagggag agcccttggt taatgcagac agaagagact ctgctgtcat
     4381 cggaggtgtg atagcagtgg tgatatttat tttgctttgc atcactgcca tagccatacg
     4441 catctatcaa cagagaaagt tacgcaaaga aaatgagtca aaagtctcaa aaaaagaaga
     4501 gtgcgatatc ggcggtggag gaagcggcgg aggtggatct atggatccac cggtcgccac
     4561 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4621 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4681 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4741 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4801 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4861 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4921 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4981 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     5041 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     5101 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     5161 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5221 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt agcggccgcg
     5281 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     5341 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     5401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     5521 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5581 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5641 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5701 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5761 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5821 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5881 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5941 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     6001 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6061 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     6121 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     6481 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6541 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6601 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6661 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     6721 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6781 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6841 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6901 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6961 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7021 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7081 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7141 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7201 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7261 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     7321 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     7381 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7441 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     7501 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     7561 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     7621 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     7681 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     7741 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7801 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7861 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7921 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7981 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8041 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8101 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8161 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8221 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8281 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8341 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8401 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8461 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8521 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8581 ggataaccgt attaccgcca tgcat
//
