LOCUS V025929 5874 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025929 VERSION V025929 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..1786 /label="APOBEC3B(NM_004900)" /note="APOBEC3B(NM_004900)" /gene="APOBEC3B" CDS 1820..2539 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2662..2783 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2790..3245) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3272..3376 /gene="bla" /label="AmpR promoter" promoter 3378..3735 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3770..4564 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4796..4843 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5172..5760 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgaatccac agatcagaaa 661 tccgatggag cggatgtatc gagacacatt ctacgacaac tttgaaaacg aacccatcct 721 ctatggtcgg agctacactt ggctgtgcta tgaagtgaaa ataaagaggg gccgctcaaa 781 tctcctttgg gacacagggg tctttcgagg ccaggtgtat ttcaagcctc agtaccacgc 841 agaaatgtgc ttcctctctt ggttctgtgg caaccagctg cctgcttaca agtgtttcca 901 gatcacctgg tttgtatcct ggaccccctg cccggactgt gtggcgaagc tggccgaatt 961 cctgtctgag caccccaatg tcaccctgac catctctgcc gcccgcctct actactactg 1021 ggaaagagat taccgaaggg cgctctgcag gctgagtcag gcaggagccc gcgtgacgat 1081 catggactat gaagaatttg catactgctg ggaaaacttt gtgtacaatg aaggtcagca 1141 attcatgcct tggtacaaat tcgatgaaaa ttatgcattc ctgcaccgca cgctaaagga 1201 gattctcaga tacctgatgg atccagacac attcactttc aactttaata atgacccttt 1261 ggtccttcga cggcgccaga cctacttgtg ctatgaggtg gagcgcctgg acaatggcac 1321 ctgggtcctg atggaccagc acatgggctt tctatgcaac gaggctaaga atcttctctg 1381 tggcttttac ggccgccatg cggagctgcg cttcttggac ctggttcctt ctttgcagtt 1441 ggacccggcc cagatctaca gggtcacttg gttcatctcc tggagcccct gcttctcctg 1501 gggctgtgcc ggggaagtgc gtgcgttcct tcaggagaac acacacgtga gactgcgcat 1561 cttcgctgcc cgcatctatg attacgaccc cctatataag gaggcgctgc aaatgctgcg 1621 ggatgctggg gcccaagtct ccatcatgac ctacgatgag tttgagtact gctgggacac 1681 ctttgtgtac cgccagggat gtcccttcca gccctgggat ggactagagg agcacagcca 1741 agccctgagt gggaggctgc gggccattct ccagaatcag ggaaacggag gtggaggttc 1801 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 1861 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 1921 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 1981 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 2041 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 2101 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 2161 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 2221 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 2281 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 2341 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 2401 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 2461 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 2521 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt 2581 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 2641 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 2701 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 2761 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt 2821 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 2881 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 2941 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 3001 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 3061 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 3121 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 3181 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 3241 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 3301 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 3361 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 3421 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3481 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3541 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 3601 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3661 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 3721 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3781 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3841 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3901 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 3961 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 4021 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 4081 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 4141 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 4201 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 4261 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 4321 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4381 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4441 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4501 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4561 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4621 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4681 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 4741 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 4801 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 4861 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 4921 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 4981 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 5041 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 5101 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 5161 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 5221 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 5281 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 5341 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 5401 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 5461 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 5521 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 5581 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 5641 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 5701 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 5761 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 5821 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat //