LOCUS       V041985                 8245 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041985
VERSION     V041985
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             937..4197
                     /label="SUPT5H(NM_003169)"
                     /note="SUPT5H(NM_003169)"
                     /gene="SUPT5H"
     CDS             4210..4275
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    4310..4534
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4580..5008
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5022..5351
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5363..5761
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     polyA_signal    5921..6042
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6091..6107)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6115..6131
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6139..6169)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6184..6205
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6493..7078)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7249..8109)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8110..8214)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcga tatcggatcc gccaccatgt cggacagcga ggacagcaac
      961 ttttccgagg aggaggacag cgagcgcagc agtgacggcg aggaggccga ggtagacgaa
     1021 gagcggcgga gtgcagcggg cagtgagaaa gaagaagagc ctgaggacga agaggaggag
     1081 gaagaggagg aggaatacga tgaggaagag gaggaagaag atgatgaccg accccccaag
     1141 aaaccccgcc atggaggctt cattctggac gaggctgatg ttgacgatga gtatgaggac
     1201 gaggaccagt gggaggatgg agcagaggac attctagaga aagaagagat tgaagcctcc
     1261 aatatcgata atgttgtcct ggatgaagat cgttctgggg ctcgccgcct gcaaaacctc
     1321 tggagggacc agcgagaaga agaactgggc gagtattaca tgaagaaata cgccaagtca
     1381 tctgtgggag agacggtgta tggaggatct gatgagctct cagacgacat cacccagcag
     1441 cagctgctcc caggagtcaa ggatcccaat ctgtggactg tcaaatgtaa gattggggag
     1501 gaacgggcca cggccatttc cttgatgcgc aagttcattg cctaccagtt cacagacacg
     1561 cccctgcaga tcaagtcagt agtggcacca gagcatgtga agggctacat ctacgtggag
     1621 gcctacaagc agacccacgt gaagcaggcc attgaggggg tgggcaacct gcggcttggc
     1681 tactggaacc agcagatggt gcccatcaag gagatgacag acgtgctcaa agtggtgaag
     1741 gaggtggcca acctgaaacc aaagtcctgg gtccgcctca agcggggcat ctacaaggat
     1801 gacattgctc aggtggacta cgtggagccc agccagaaca ccatctccct gaagatgatc
     1861 ccacgcatcg actacgatcg catcaaggcc cgcatgagct tgaaagactg gtttgccaaa
     1921 aggaagaagt ttaagcggcc tccacagagg ctgtttgatg ctgagaagat caggtccctg
     1981 gggggtgatg ttgcctctga tggtgacttc ctcatctttg aggggaaccg ttacagccgg
     2041 aagggctttc tgttcaagag cttcgccatg tctgctgtga tcacggaggg tgtgaagcca
     2101 acactctctg agctggaaaa gtttgaggac cagccagagg gcattgacct ggaggtggtg
     2161 actgagagca cagggaagga gcgggagcac aacttccaac ctggggacaa cgtggaggtc
     2221 tgtgagggtg agctcatcaa cctgcagggc aagatcctca gcgtggatgg caacaagatc
     2281 accatcatgc ccaagcatga ggacctcaag gacatgttgg agttcccagc ccaggaactt
     2341 agaaaatact tcaagatggg ggaccacgtg aaggtgattg ctggccgatt cgagggcgac
     2401 acaggcctca ttgtgcgggt ggaggagaat ttcgttatcc tgttctctga cctcaccatg
     2461 catgagctga aggtgctccc ccgggacctg cagctctgct cagagacagc atcaggtgtg
     2521 gatgttgggg gccagcatga atggggcgag ctggtgcagc tggatcccca gactgtgggt
     2581 gtcatcgtgc gactagaacg ggagaccttc caggtgctga acatgtacgg gaaggtggtg
     2641 actgtcagac atcaggctgt gacccggaag aaggacaacc gctttgctgt ggccttggac
     2701 tcagagcaga acaacatcca tgtgaaagac atcgttaagg tcattgatgg cccccactca
     2761 ggccgagaag gggagattcg ccatctcttc cgaagcttcg ccttcctaca ttgcaagaaa
     2821 ctggtggaga acgggggcat gtttgtctgc aagacccgcc acctggtgct ggctgggggc
     2881 tcaaagcccc gtgatgtgac caacttcacc gtgggtggct ttgcgcctat gagtccccgg
     2941 atcagcagcc ccatgcaccc cagtgctgga ggtcagcgtg gcggctttgg tagcccaggt
     3001 ggcggcagtg gtggcatgag caggggccgg ggccggaggg acaacgaact catcggccag
     3061 accgtgcgca tctcccaggg gccctacaaa ggctacatcg gtgtggtgaa agatgccaca
     3121 gagtccacgg cccgtgtgga gctgcactcc acctgccaga ccatctctgt ggaccgtcag
     3181 cggctcacca cggtgggctc acggcgcccg ggcggcatga cctcgaccta tgggaggacg
     3241 cccatgtatg gctcccagac gcccatgtat ggctctggct cccgaacacc catgtacggc
     3301 tcacagacac ccctccagga tggtagccgc accccacact acggctcaca gacgcccctg
     3361 catgatggca gccgcactcc tgcccagagt ggggcctggg accccaacaa ccccaacacg
     3421 ccgtcacggg ctgaggaaga atatgagtat gctttcgatg atgagcccac cccgtccccg
     3481 caggcctatg ggggaacccc caatccccaa acacctggct acccagaccc ctcgtcccca
     3541 caggtcaacc cacaatacaa cccgcagacg ccagggacgc cggccatgta caacacagac
     3601 cagttctctc cctatgctgc cccctcccca caaggttcct accagcccag ccccagcccc
     3661 cagagctacc accaggtggc gccaagccca gcaggctacc agaataccca ctccccagcc
     3721 agctaccacc ctacaccgtc gcccatggcc tatcaggcta gccccagccc gagccccgtt
     3781 ggctacagtc ctatgacacc tggagctccc tcccctggtg gctacaaccc acacacgcca
     3841 ggctcaggca tcgagcagaa ctccagcgac tgggtaacca ctgacattca ggtgaaggtg
     3901 cgggacacct acctggatac acaggtggtg ggacagacag gtgtcatccg cagtgtcacg
     3961 gggggcatgt gctctgtgta cctgaaggac agtgagaagg ttgtcagcat ttccagtgag
     4021 cacctggagc ctatcacccc caccaagaac aacaaggtga aagtgatcct gggcgaggat
     4081 cgggaagcca cgggcgtcct actgagcatt gatggtgagg atggcattgt ccgtatggac
     4141 cttgatgagc agctcaagat cctcaacctc cgcttcctgg ggaagctcct ggaagccctc
     4201 gagaccggtg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     4261 gatgacgatg acaagtgacc ggtaagttta aaccgctgat cagcctcgac tgtgccttct
     4321 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4381 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4441 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4501 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4561 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4621 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4681 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4741 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4801 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4861 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4921 gtctattctt ttgatttata agggattttg gccatttcgg cctattggtt aaaaaatgag
     4981 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5041 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5101 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5161 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5221 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5281 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5341 gcttttgcaa aaagctcccg ggatggccaa gcctttgtct caagaagaat ccaccctcat
     5401 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     5461 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     5521 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     5581 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5641 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5701 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     5761 attcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5821 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5881 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     6061 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6121 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6181 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6241 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6301 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6361 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6901 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6961 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7021 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7681 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8221 tccccgaaaa gtgccacctg acgtc
//
