LOCUS       V018225                 6750 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018225
VERSION     V018225
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1036..3051
                     /label="SLC5A2(NM_003041)"
                     /note="SLC5A2(NM_003041)"
                     /gene="SLC5A2"
     primer_bind     complement(3078..3094)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3105..3727
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     promoter        3756..4085
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4124..4142)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(4156..4172)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4180..4196
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4204..4234)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4249..4270
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4558..5146)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5317..6177)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6178..6282)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6309..6737
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccgcga attcgagctc ggtacctcgc gaatgcatct agatatcgga
     1021 tcccgacacg aattcatgga ggagcacaca gaggcaggct cggcaccaga gatgggggcc
     1081 cagaaggccc tgattgacaa tcctgctgac atcctagtca ttgctgcata tttcctgctg
     1141 gtcattggcg ttggcttgtg gtccatgtgc agaaccaaca gaggcactgt gggcggctac
     1201 ttcctggcag gacgcagcat ggtgtggtgg ccggttgggg cctctctctt cgccagcaac
     1261 atcggcagtg gccactttgt gggcctggca gggactggcg ctgcaagtgg cttggctgtt
     1321 gctggattcg agtggaatgc gctcttcgtg gtgctgctac tgggctggct gtttgcaccc
     1381 gtgtacctga cagcgggggt catcacgatg ccacagtacc tgcgcaagcg cttcggcggc
     1441 cgccgcatcc gcctctacct gtctgtgctc tcccttttcc tgtacatctt caccaagatc
     1501 tcagtggaca tgttctccgg agctgtattc atccagcagg ctctgggctg gaacatctat
     1561 gcctccgtca tcgcgcttct gggcatcacc atgatttaca cggtgacagg agggctggcc
     1621 gcgctgatgt acacggacac ggtacagacc ttcgtcattc tggggggcgc ctgcatcctc
     1681 atgggttacg ccttccacga ggtgggcggg tattcgggtc tcttcgacaa atacctggga
     1741 gcagcgactt cgctgacggt gtccgaggat ccagccgtgg gaaacatctc cagcttctgc
     1801 tatcgacccc ggcccgactc ctaccacctg ctccggcacc ccgtgaccgg ggatctgccg
     1861 tggcccgcgc tgctcctcgg actcacaatc gtctcgggct ggtactggtg cagcgaccag
     1921 gtcatcgtgc agcgctgcct ggccgggaag agcctgaccc acatcaaggc gggctgcatc
     1981 ctgtgtgggt acctgaagct gacgcccatg tttctcatgg tcatgccagg catgatcagc
     2041 cgcattctgt acccagacga ggtggcgtgc gtggtgcctg aggtgtgcag gcgcgtgtgc
     2101 ggcacggagg tgggctgctc caacatcgcc tacccgcggc tcgtcgtgaa gctcatgccc
     2161 aacggtctgc gcggactcat gctggcggtc atgctggccg cgctcatgtc ctcgctggcc
     2221 tccatcttca acagcagcag cacgctcttc accatggaca tctacacgcg cctgcggcca
     2281 cgcgccggcg accgcgagct gctgctggtg ggacggctct gggtggtgtt catcgtggta
     2341 gtgtcggtgg cctggcttcc cgtggtgcag gcggcacagg gcgggcagct cttcgattac
     2401 atccaggcag tctctagcta cctggcaccg cccgtgtccg ccgtcttcgt gctggcgctc
     2461 ttcgtgccgc gcgttaatga gcagggcgcc ttctggggac tcatcggggg cctgctgatg
     2521 ggcctggcac gcctgattcc cgagttctcc ttcggctcgg gcagctgtgt gcagccctcg
     2581 gcgtgcccag ctttcctctg cggcgtgcac tacctctact tcgccattgt gctgttcttc
     2641 tgctctggcc tcctcaccct cacggtctcc ctgtgcaccg cgcccatccc cagaaagcac
     2701 ctccaccgcc tggtcttcag tctccggcat agcaaggagg aacgggagga cctggatgct
     2761 gatgagcagc aaggctcctc actccctgta cagaatgggt gcccagagag tgccatggag
     2821 atgaatgagc cccaggcccc ggcaccaagc ctcttccgcc agtgcctgct ctggttttgt
     2881 ggaatgagca gaggtggggt gggcagtcct ccgcccctta cccaggagga ggcagcggca
     2941 gcagccaggc ggctggagga catcagcgag gacccgagct gggcccgtgt ggtcaacctc
     3001 aatgccctgc tcatgatggc agtggccgtg ttcctctggg gcttctatgc ctaactcgac
     3061 tctagattgc ggccgcggtc atagctgttt cctgaacaga tcccgggtgg catccctgtg
     3121 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     3181 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     3241 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     3301 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     3361 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     3421 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     3481 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     3541 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat
     3601 accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac
     3661 atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg
     3721 ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3781 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3841 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3901 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3961 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4021 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4081 gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa ttccctatag tgagtcgtat
     4141 taaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6301 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6361 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6421 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6481 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6541 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6601 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6661 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6721 aaaatttaac gcgaatttta acaaaatatt
//
