LOCUS       V041547                 6541 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041547
VERSION     V041547
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     141..157
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        318..697
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        698..901
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             931..996
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             1015..2871
                     /label="SLC27A6(NM_014031)"
                     /note="SLC27A6(NM_014031)"
                     /gene="SLC27A6"
     polyA_signal    2882..3504
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     promoter        3533..3862
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(3901..3919)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(3933..3949)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3957..3973
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3981..4011)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4026..4047
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4335..4923)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5094..5954)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5955..6059)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6086..6514
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcgatgctt
     1021 ctgtcatggc taacagttct aggggctgga atggtcgtcc tgcacttctt gcagaaactc
     1081 ctgttccctt acttttggga tgacttctgg ttcgtgttga aggtggtgct cattataatt
     1141 cggctgaaga agtatgaaaa gagaggggag ctggtgactg tgctggataa attcttgagt
     1201 catgccaaaa gacaacctcg gaaacctttc atcatctatg agggagacat ctacacctat
     1261 caggatgtag acaaaaggag cagcagagtg gcccatgtct tcctgaacca ttcctctctg
     1321 aaaaaggggg acacggtggc tctgctgatg agcaatgagc cggacttcgt tcacgtgtgg
     1381 ttcggcctcg ccaagctggg ctgcgtggtg gcctttctca acaccaacat tcgctccaac
     1441 tccctcctga attgcatccg cgcctgtggg cccagagccc tagtggtggg cgcagatttg
     1501 cttggaacgg tagaagaaat ccttccaagc ctctcagaaa atatcagtgt ttgggggatg
     1561 aaagattctg ttccacaagg tgtaatttca ctcaaagaaa aactgagcac ctcacctgat
     1621 gagcccgtgc cacgcagcca ccatgttgtc tcactcctca agtctacttg tctttacatt
     1681 tttacctctg gaacaacagg tctaccaaaa gcagctgtga ttagtcagct gcaggtttta
     1741 aggggttctg ctgtcctgtg ggcttttggt tgtactgctc atgacattgt ttatataacc
     1801 cttcctctgt atcatagttc agcagctatc ctgggaattt ctggatgtgt tgagttgggt
     1861 gccacttgtg tgttaaagaa gaaattttca gcaagccagt tttggagtga ctgcaagaag
     1921 tatgatgtga ctgtgtttca gtatattgga gaactttgtc gctacctttg caaacaatct
     1981 aagagagaag gagaaaagga tcataaggtg cgtttggcaa ttggaaatgg catacggagt
     2041 gatgtatgga gagaattttt agacagattt ggaaatataa aggtgtgtga actttatgca
     2101 gctaccgaat caagcatatc tttcatgaac tacactggga gaattggagc aattgggaga
     2161 acaaatttgt tttacaaact tctttccact tttgacttaa taaagtatga ctttcagaaa
     2221 gatgaaccca tgagaaatga gcagggttgg tgtattcatg tgaaaaaagg agaacctgga
     2281 cttctcattt ctcgagtgaa tgcaaaaaat cccttctttg gctatgctgg gccttataag
     2341 cacacaaaag acaaattgct ttgtgatgtt tttaagaagg gagatgttta ccttaatact
     2401 ggagacttaa tagtccagga tcaggacaat ttcctttatt tttgggaccg tactggagac
     2461 actttcagat ggaaaggaga aaatgtcgca accactgagg ttgctgatgt tattggaatg
     2521 ttggatttca tacaggaagc aaacgtctat ggtgtggcta tatcaggtta tgaaggaaga
     2581 gcaggaatgg cttctattat tttaaaacca aatacatctt tagatttgga aaaagtttat
     2641 gaacaagttg taacatttct accagcttat gcttgtccac gatttttaag aattcaggaa
     2701 aaaatggaag caacaggaac attcaaacta ttgaagcatc agttggtgga agatggattt
     2761 aatccactga aaatttctga accactttac ttcatggata acttgaaaaa gtcttatgtt
     2821 ctactgacca gggaacttta tgatcaaata atgttagggg aaataaaact ttaaggatcc
     2881 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     2941 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     3001 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     3061 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     3121 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     3181 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     3241 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     3301 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     3361 gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca
     3421 tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc
     3481 cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca
     3541 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3601 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3661 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3721 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3781 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3841 tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga aagttaattc
     3901 cctatagtga gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     3961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4021 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6061 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6541 t
//
