LOCUS V041547 6541 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041547 VERSION V041547 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 141..157 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 318..697 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 698..901 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 931..996 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" CDS 1015..2871 /label="SLC27A6(NM_014031)" /note="SLC27A6(NM_014031)" /gene="SLC27A6" polyA_signal 2882..3504 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" promoter 3533..3862 /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(3901..3919) /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" primer_bind complement(3933..3949) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3957..3973 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3981..4011) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4026..4047 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4335..4923) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5094..5954) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5955..6059) /gene="bla" /label="AmpR promoter" rep_origin 6086..6514 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa 961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcgatgctt 1021 ctgtcatggc taacagttct aggggctgga atggtcgtcc tgcacttctt gcagaaactc 1081 ctgttccctt acttttggga tgacttctgg ttcgtgttga aggtggtgct cattataatt 1141 cggctgaaga agtatgaaaa gagaggggag ctggtgactg tgctggataa attcttgagt 1201 catgccaaaa gacaacctcg gaaacctttc atcatctatg agggagacat ctacacctat 1261 caggatgtag acaaaaggag cagcagagtg gcccatgtct tcctgaacca ttcctctctg 1321 aaaaaggggg acacggtggc tctgctgatg agcaatgagc cggacttcgt tcacgtgtgg 1381 ttcggcctcg ccaagctggg ctgcgtggtg gcctttctca acaccaacat tcgctccaac 1441 tccctcctga attgcatccg cgcctgtggg cccagagccc tagtggtggg cgcagatttg 1501 cttggaacgg tagaagaaat ccttccaagc ctctcagaaa atatcagtgt ttgggggatg 1561 aaagattctg ttccacaagg tgtaatttca ctcaaagaaa aactgagcac ctcacctgat 1621 gagcccgtgc cacgcagcca ccatgttgtc tcactcctca agtctacttg tctttacatt 1681 tttacctctg gaacaacagg tctaccaaaa gcagctgtga ttagtcagct gcaggtttta 1741 aggggttctg ctgtcctgtg ggcttttggt tgtactgctc atgacattgt ttatataacc 1801 cttcctctgt atcatagttc agcagctatc ctgggaattt ctggatgtgt tgagttgggt 1861 gccacttgtg tgttaaagaa gaaattttca gcaagccagt tttggagtga ctgcaagaag 1921 tatgatgtga ctgtgtttca gtatattgga gaactttgtc gctacctttg caaacaatct 1981 aagagagaag gagaaaagga tcataaggtg cgtttggcaa ttggaaatgg catacggagt 2041 gatgtatgga gagaattttt agacagattt ggaaatataa aggtgtgtga actttatgca 2101 gctaccgaat caagcatatc tttcatgaac tacactggga gaattggagc aattgggaga 2161 acaaatttgt tttacaaact tctttccact tttgacttaa taaagtatga ctttcagaaa 2221 gatgaaccca tgagaaatga gcagggttgg tgtattcatg tgaaaaaagg agaacctgga 2281 cttctcattt ctcgagtgaa tgcaaaaaat cccttctttg gctatgctgg gccttataag 2341 cacacaaaag acaaattgct ttgtgatgtt tttaagaagg gagatgttta ccttaatact 2401 ggagacttaa tagtccagga tcaggacaat ttcctttatt tttgggaccg tactggagac 2461 actttcagat ggaaaggaga aaatgtcgca accactgagg ttgctgatgt tattggaatg 2521 ttggatttca tacaggaagc aaacgtctat ggtgtggcta tatcaggtta tgaaggaaga 2581 gcaggaatgg cttctattat tttaaaacca aatacatctt tagatttgga aaaagtttat 2641 gaacaagttg taacatttct accagcttat gcttgtccac gatttttaag aattcaggaa 2701 aaaatggaag caacaggaac attcaaacta ttgaagcatc agttggtgga agatggattt 2761 aatccactga aaatttctga accactttac ttcatggata acttgaaaaa gtcttatgtt 2821 ctactgacca gggaacttta tgatcaaata atgttagggg aaataaaact ttaaggatcc 2881 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc 2941 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc 3001 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac 3061 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg 3121 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt 3181 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg 3241 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc 3301 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct 3361 gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca 3421 tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc 3481 cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca 3541 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3601 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3661 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 3721 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 3781 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 3841 tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga aagttaattc 3901 cctatagtga gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt 3961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 4021 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 4081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 4141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 4561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 4801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 4921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 4981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 5041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 6001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6061 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 6121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 6181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 6241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 6301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 6361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 6421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 6481 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt 6541 t //