LOCUS       V020408                 7971 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020408
VERSION     V020408
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             991..4254
                     /label="LATS2(NM_014572)"
                     /note="LATS2(NM_014572)"
                     /gene="LATS2"
     CDS             4255..4278
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(4299..4315)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    4326..4948
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     promoter        4977..5306
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5345..5363)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(5377..5393)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5401..5417
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5425..5455)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5470..5491
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5779..6367)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6538..7398)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7399..7503)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      7530..7958
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccgcga attcgccacc atgaggccaa agacttttcc tgccacgact
     1021 tattctggaa atagccggca gcgactgcaa gagattcgtg aggggttaaa acagccatcc
     1081 aagtcttcgg ttcaggggct acccgcagga ccaaacagtg acacttccct ggatgccaaa
     1141 gtcctgggga gcaaagatgc caccaggcag cagcagcaga tgagagccac cccaaagttc
     1201 ggaccttatc agaaagcctt gagggaaatc agatattcct tgttgccttt tgctaatgaa
     1261 tcgggcacct ctgcagctgc agaagtgaac cggcaaatgc tgcaggaact ggtgaacgca
     1321 ggatgcgacc aggagatggc tggccgagct ctcaagcaga ctggcagcag gagcatcgag
     1381 gccgccctgg agtacatcag caagatgggc tacctggacc cgaggaatga gcagattgtg
     1441 cgggtcatta agcagacctc cccaggaaag gggctcatgc caaccccagt gacgcggagg
     1501 cccagcttcg aaggaaccgg cgattcgttt gcgtcctacc accagctgag cggtaccccc
     1561 tacgagggcc caagcttcgg cgctgacggc cccacggcgc tggaggagat gccgcggccg
     1621 tacgtggact accttttccc cggagtcggc ccccacgggc ccggccacca gcaccagcac
     1681 ccacccaagg gctacggtgc cagcgtagag gcagcagggg cacacttccc gctgcagggc
     1741 gcgcactacg ggcggccgca cctgctggtg cctggggaac ccctgggcta cggagtgcag
     1801 cgcagcccct ccttccagag caagacgccg ccggagaccg ggggttacgc cagcctgccc
     1861 acgaagggcc agggaggacc gccaggcgcc ggcctcgctt tcccaccccc tgccgccggg
     1921 ctctacgtgc cgcacccaca ccacaagcag gccggtcccg cggcccacca gctgcatgtg
     1981 ctgggctccc gcagccaggt gttcgccagc gacagccccc cgcagagcct gctcactccc
     2041 tcgcggaaca gcctcaacgt ggacctgtat gaattgggca gcacctccgt ccagcagtgg
     2101 ccggctgcca ccctggcccg ccgggactcc ctgcagaagc cgggcctgga ggcgccgccg
     2161 cgcgcgcacg tggccttccg gcctgactgc ccagtgccca gcaggaccaa ctccttcaac
     2221 agccaccagc cgcggcccgg tccgcctggc aaggccgagc cctccctgcc cgcccccaac
     2281 accgtgacgg ctgtcacggc cgcgcacatc ttgcacccgg tgaagagcgt gcgtgtgctg
     2341 aggccggagc cgcagacggc tgtggggccc tcgcaccccg cctgggtgcc cgcgcctgcc
     2401 ccggcccccg cccccgcccc cgccccggct gcggagggct tggacgccaa ggaggagcat
     2461 gccctggcgc tgggcggcgc aggcgccttc ccgctggacg tggagtacgg aggcccagac
     2521 cggaggtgcc cgcctccgcc ctacccgaag cacctgctgc tgcgcagcaa gtcggagcag
     2581 tacgacctgg acagcctgtg cgcaggcatg gagcagagcc tccgtgcggg ccccaacgag
     2641 cccgagggcg gcgacaagag ccgcaaaagc gccaaggggg acaaaggcgg aaaggataaa
     2701 aagcagattc agacctctcc cgttcccgtc cgcaaaaaca gcagagacga agagaagaga
     2761 gagtcacgca tcaagagcta ctcgccatac gcctttaagt tcttcatgga gcagcacgtg
     2821 gagaatgtca tcaaaaccta ccagcagaag gttaaccgga ggctgcagct ggagcaagaa
     2881 atggccaaag ctggactctg tgaagctgag caggagcaga tgcggaagat cctctaccag
     2941 aaagagtcta attacaacag gttaaagagg gccaagatgg acaagtctat gtttgtcaag
     3001 atcaaaaccc tggggatcgg tgcctttgga gaagtgtgcc ttgcttgtaa ggtggacact
     3061 cacgccctgt acgccatgaa gaccctaagg aaaaaggatg tcctgaaccg gaatcaggtg
     3121 gcccacgtca aggccgagag ggacatcctg gccgaggcag acaatgagtg ggtggtcaaa
     3181 ctctactact ccttccaaga caaagacagc ctgtactttg tgatggacta catccctggt
     3241 ggggacatga tgagcctgct gatccggatg gaggtcttcc ctgagcacct ggcccggttc
     3301 tacatcgcag agctgacttt ggccattgag agtgtccaca agatgggctt catccaccga
     3361 gacatcaagc ctgataacat tttgatagat ctggatggtc acattaaact cacagatttc
     3421 ggcctctgca ctgggttcag gtggactcac aattccaaat attaccagaa agggagccat
     3481 gtcagacagg acagcatgga gcccagcgac ctctgggatg atgtgtctaa ctgtcggtgt
     3541 ggggacaggc tgaagaccct agagcagagg gcgcggaagc agcaccagag gtgcctggca
     3601 cattcactgg tggggactcc aaactacatc gcacccgagg tgctcctccg caaagggtac
     3661 actcaactct gtgactggtg gagtgttgga gtgattctct tcgagatgct ggtggggcag
     3721 ccgccctttt tggcacctac tcccacagaa acccagctga aggtgatcaa ctgggagaac
     3781 acgctccaca ttccagccca ggtgaagctg agccctgagg ccagggacct catcaccaag
     3841 ctgtgctgct ccgcagacca ccgcctgggg cggaatgggg ccgatgacct gaaggcccac
     3901 cccttcttca gcgccattga cttctccagt gacatccgga agcagccagc cccctacgtt
     3961 cccaccatca gccaccccat ggacacctcg aatttcgacc ccgtagatga agaaagccct
     4021 tggaacgatg ccagcgaagg tagcaccaag gcctgggaca cactcacctc gcccaataac
     4081 aagcatcctg agcacgcatt ttacgaattc accttccgaa ggttctttga tgacaatggc
     4141 tacccctttc gatgcccaaa gccttcagga gcagaagctt cacaggctga gagctcagat
     4201 ttagaaagct ctgatctggt ggatcagact gaaggctgcc agcctgtgta cgtggattac
     4261 aaggacgacg atgacaagta gtctagattg cggccgcggt catagctgtt tcctgaacag
     4321 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     4381 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     4441 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     4501 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     4561 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     4621 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     4681 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     4741 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     4801 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
     4861 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
     4921 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt
     4981 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5041 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5101 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5161 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5221 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5281 tttttggagg cctaggcttt tgcaaaaagc tcctcgagga actgaaaaac cagaaagtta
     5341 attccctata gtgagtcgta ttaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     5401 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5461 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5521 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5581 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5941 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6181 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6241 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6301 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6361 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6421 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6481 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6541 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6601 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6661 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6721 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6781 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6841 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6901 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6961 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7021 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7081 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7141 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7201 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7261 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7321 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7381 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7441 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7501 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     7561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7741 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7861 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7921 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat t
//
