LOCUS       V040614                 5335 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040614
VERSION     V040614
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             841..870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             895..2448
                     /label="RIPK3(NM_006871)"
                     /note="RIPK3(NM_006871)"
                     /gene="RIPK3"
     polyA_signal    2474..2608
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2719..2735)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2743..2759
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2767..2797)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2812..2833
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3121..3709)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3880..4740)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4741..4845)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5319..5335
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 gagcagaagc tgatctcaga ggaggacctg cttatggcca tggaggcccg aattatgtcg
      901 tgcgtcaagt tatggcccag cggtgccccc gcccccttgg tgtccatcga ggaactggag
      961 aaccaggagc tcgtcggcaa aggcgggttc ggcacagtgt tccgggcgca acataggaag
     1021 tggggctacg atgtggcggt caagatcgta aactcgaagg cgatatccag ggaggtcaag
     1081 gccatggcaa gtctggataa cgaattcgtg ctgcgcctag aaggggttat cgagaaggtg
     1141 aactgggacc aagatcccaa gccggctctg gtgactaaat tcatggagaa cggctccttg
     1201 tcggggctgc tgcagtccca gtgccctcgg ccctggccgc tcctttgccg cctgctgaaa
     1261 gaagtggtgc ttgggatgtt ttacctgcac gaccagaacc cggtgctcct gcaccgggac
     1321 ctcaagccat ccaacgtcct gctggaccca gagctgcacg tcaagctggc agattttggc
     1381 ctgtccacat ttcagggagg ctcacagtca gggacagggt ccggggagcc agggggcacc
     1441 ctgggctact tggccccaga actgtttgtt aacgtaaacc ggaaggcctc cacagccagt
     1501 gacgtctaca gcttcgggat cctaatgtgg gcagtgcttg ctggaagaga agttgagttg
     1561 ccaaccgaac catcactcgt gtacgaagca gtgtgcaaca ggcagaaccg gccttcattg
     1621 gctgagctgc cccaagccgg gcctgagact cccggcttag aaggactgaa ggagctaatg
     1681 cagctctgct ggagcagtga gcccaaggac agaccctcct tccaggaatg cctaccaaaa
     1741 actgatgaag tcttccagat ggtggagaac aatatgaatg ctgctgtctc cacggtaaag
     1801 gatttcctgt ctcagctcag gagcagcaat aggagatttt ctatcccaga gtcaggccaa
     1861 ggagggacag aaatggatgg ctttaggaga accatagaaa accagcactc tcgtaatgat
     1921 gtcatggttt ctgagtggct aaacaaactg aatctagagg agcctcccag ctctgttcct
     1981 aaaaaatgcc cgagccttac caagaggagc agggcacaag aggagcaggt tccacaagcc
     2041 tggacagcag gcacatcttc agattcgatg gcccaacctc cccagactcc agagacctca
     2101 actttcagaa accagatgcc cagccctacc tcaactggaa caccaagtcc tggaccccga
     2161 gggaatcagg gggctgagag acaaggcatg aactggtcct gcaggacccc ggagccaaat
     2221 ccagtaacag ggcgaccgct cgttaacata tacaactgct ctggggtgca agttggagac
     2281 aacaactact tgactatgca acagacaact gccttgccca catggggctt ggcaccttcg
     2341 ggcaagggga ggggcttgca gcacccccca ccagtaggtt cgcaagaagg ccctaaagat
     2401 cctgaagcct ggagcaggcc acagggttgg tataatcata gcgggaaata actcgaggta
     2461 ccgcggccgc ggggatccag acatgataag atacattgat gagtttggac aaaccacaac
     2521 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2581 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2641 ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct gcaggcatgc
     2701 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2761 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2821 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2881 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2941 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3001 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3061 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3121 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3181 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3301 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3421 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3481 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3541 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3601 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4141 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4201 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4261 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4321 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4381 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4441 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4501 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4561 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4621 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4681 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4741 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4801 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4861 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4921 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4981 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5041 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5101 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5161 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5221 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     5281 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagt
//
