LOCUS V025538 7868 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025538 VERSION V025538 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 872..1579 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1616..3499 /label="SNX18(NM_052870)" /note="SNX18(NM_052870)" /gene="SNX18" CDS 3506..3532 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3536..3562 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3566..3592 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 3630..3854 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3900..4328 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4342..4671 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4738..5337 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 5511..5632 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5681..5697) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5705..5721 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5729..5759) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5774..5795 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6083..6668) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6839..7699) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7700..7804) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagccac catggtgagc aagggcgagg aggataacat 901 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 961 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 1021 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 1081 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 1141 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 1201 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg 1261 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 1321 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 1381 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 1441 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 1501 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 1561 catggacgag ctgtacaagg gaggtggagg tagtggagga ggcggatcaa gcttcatggc 1621 gctgcgcgcc cgggcgctgt acgacttcag gtcggagaac ccaggagaga tctcgctgcg 1681 agagcacgag gtgctgagcc tgtgcagcga gcaggacatc gagggctggc tcgagggggt 1741 caacagccgc ggcgaccgcg gcctcttccc ggcctcctat gtgcaggtga tccgcgcccc 1801 cgagcctggc ccggcgggag acggcggccc gggcgccccg gcccgctacg ccaatgtgcc 1861 ccccgggggc ttcgagcccc tgcctgtcgc gccccccgcc tccttcaagc cgccgcctga 1921 cgccttccag gcgctgctgc agccacagca ggcgccgcct ccgagcacct tccagccgcc 1981 cggcgcgggc ttcccgtacg gcgggggcgc cctgcagccg tcgcctcagc agctctacgg 2041 cggctaccag gccagccaag gcagcgatga tgactgggac gacgagtggg acgacagctc 2101 cacggtggcg gacgagccgg gcgctctggg cagcggagca tacccggacc tcgacggctc 2161 gtcttcggcg ggtgtgggcg cagccggccg ctaccgcctg tccacgcgct ccgacctgtc 2221 cctgggttcc cgcggcggct cggtcccccc gcagcaccac ccgtcggggc ccaagagctc 2281 ggccaccgtg agccgcaacc tcaatcgctt ctccaccttc gtcaagtccg gcggggaggc 2341 cttcgtgctg ggggaggcgt caggcttcgt gaaggacggg gacaagctgt gcgtggtgct 2401 ggggccctat ggccccgagt ggcaggagaa cccctacccg ttccagtgca ccatcgacga 2461 ccccaccaag cagaccaagt tcaagggcat gaagagctac atctcctaca agctggtgcc 2521 cacgcacacg caggtgccgg tgcatcggcg ctacaagcac ttcgactggc tgtacgcgcg 2581 cctggcggag aagttcccgg tcatctccgt gccccacctg cccgagaagc aggccaccgg 2641 ccgcttcgag gaggacttca tctctaagcg caggaagggc ctgatctggt ggatgaacca 2701 catggccagc cacccagtgc tggcgcagtg cgacgtcttc cagcacttcc tgacgtgccc 2761 cagcagcacc gacgagaaag cctggaagca gggcaagagg aaggccgaga aggacgagat 2821 ggtgggcgcc aacttcttcc tgacccttag cacgcccccc gccgctgccc ttgacctgca 2881 ggaggtggag agcaagatcg acggcttcaa gtgcttcacc aagaagatgg acgacagcgc 2941 gctgcagctc aaccacacgg ccaacgagtt cgcgcgcaag caggtgaccg gcttcaaaaa 3001 ggagtatcag aaggtgggcc agtccttccg cggcctcagc caggcctttg agctggacca 3061 gcaggccttc tcggtgggcc tgaaccaggc tatcgccttc accggagatg cctatgacgc 3121 cattggcgag ctcttcgcgg agcagcccag gcaggacctg gatcccgtca tggacctatt 3181 agcgctgtat caggggcatc tggctaactt cccggacatc atccacgttc agaaaggtaa 3241 agcctggccc ttagagcagg tgatatggag tgtattgtgc aggctgaaag gggcgacttt 3301 gacagcagta ccactgtggg tttcagaatc atattctaca ggtgaggaag cgagcagaga 3361 cgtggacgcc tgggtctttt ccctagagtg taagttggat tgctcgacag gcagcttcct 3421 cctcgagtat cttgcattag ggaatgagta ctctttctcg aaggttcaaa gagtaccttt 3481 gatgacagtg ctatcatttc tcgagtaccc atacgacgta cctgattatg caacttatcc 3541 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg catagtgagg 3601 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 3661 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 3721 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 3781 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 3841 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 3901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 3961 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 4021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 4081 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 4141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 4201 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 4261 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 4321 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4381 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4501 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4561 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 4621 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 4681 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 4741 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc 4801 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc 4861 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 4921 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 4981 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5041 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5101 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5161 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5221 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5281 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg 5341 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 5401 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 5461 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 5521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5641 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 5701 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 5761 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 5821 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5881 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 5941 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 6001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 6061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 6121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 6181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 6241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 6301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 6361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 6421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 6541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 6601 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 7021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7801 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 7861 cctatggt //