LOCUS       V039138                 7370 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039138
VERSION     V039138
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..2998
                     /label="PRKCG(NM_002739)"
                     /note="PRKCG(NM_002739)"
                     /gene="PRKCG"
     CDS             3005..3031
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3035..3061
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3065..3091
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3132..3356
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3402..3830
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3844..4173
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4240..4839
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    5013..5134
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5183..5199)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5207..5223
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5231..5261)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5276..5297
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5585..6170)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6341..7201)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7202..7306)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gctggtctgg gccccggcgt aggcgattca gaggggggac cccggcccct
      961 gttttgcaga aagggggccc tgaggcagaa ggtggtccac gaagtcaaga gccacaagtt
     1021 caccgctcgc ttcttcaagc agcccacctt ctgcagccac tgcaccgact tcatctgggg
     1081 tatcggaaag cagggcctgc aatgtcaagt ctgcagcttt gtggttcatc gacgatgcca
     1141 cgaatttgtg accttcgagt gtccaggcgc tgggaagggc ccccagacgg acgacccccg
     1201 gaacaaacac aagttccgcc tgcatagcta cagcagcccc accttctgcg accactgtgg
     1261 ctccctcctc tacgggcttg tgcaccaggg catgaaatgc tcctgctgcg agatgaacgt
     1321 gcaccggcgc tgtgtgcgta gcgtgccctc cctgtgcggt gtggaccaca ccgagcgccg
     1381 cgggcgcctg cagctggaga tccgggctcc cacagcagat gagatccacg taactgttgg
     1441 cgaggcccgt aacctaattc ctatggaccc caatggtctc tctgatccct atgtgaaact
     1501 gaagctcatc ccagaccctc ggaacctgac gaaacagaag acccgaacgg tgaaagccac
     1561 gctaaaccct gtgtggaatg agacctttgt gttcaacctg aagccagggg atgtggagcg
     1621 ccggctcagc gtggaggtgt gggactggga ccggacctcc cgcaacgact tcatgggggc
     1681 catgtccttt ggcgtctcgg agctgctcaa ggcgcccgtg gatggctggt acaagttact
     1741 gaaccaggag gagggcgagt attacaatgt gccggtggcc gatgctgaca actgcagcct
     1801 cctccagaag tttgaggctt gtaactaccc cctggaattg tatgagcggg tgcggatggg
     1861 cccctcttcc tctcccatcc cctccccttc ccctagtccc accgacccca agcgctgctt
     1921 cttcggggcg agtccaggac gcctgcacat ctccgacttc agcttcctca tggttctagg
     1981 aaaaggcagt tttgggaagg tgatgctggc cgagcgcagg ggctctgatg agctctacgc
     2041 catcaagatc ttgaaaaagg acgtgatcgt ccaggacgac gatgtggact gcacgctggt
     2101 ggagaaacgt gtgctggcgc tggggggccg gggtcctggc ggccggcccc acttcctcac
     2161 ccagctccac tccaccttcc agaccccgga ccgcctgtat ttcgtgatgg agtacgtcac
     2221 cgggggagac ttgatgtacc acattcaaca gctgggcaag tttaaggagc cccatgcagc
     2281 gttctacgcg gcagaaatcg ctatcggcct cttcttcctt cacaatcagg gcatcatcta
     2341 cagggacctg aagctggaca atgtgatgct ggatgctgag ggacacatca agatcactga
     2401 ctttggcatg tgtaaggaga acgtcttccc cgggacgaca acccgcacct tctgcgggac
     2461 cccggactac atagccccgg agatcattgc ctaccagccc tatgggaagt ctgtcgattg
     2521 gtggtccttt ggagttctgc tgtatgagat gttggcagga cagcctccct tcgatgggga
     2581 ggacgaggag gagctgtttc aggccatcat ggaacaaact gtcacctacc ccaagtcgct
     2641 ttcccgggaa gccgtggcca tctgcaaggg gttcctgacc aagcacccag ggaagcgcct
     2701 gggctcaggg cctgatgggg aacctaccat ccgtgcacat ggctttttcc gctggattga
     2761 ctgggagcgg ctggaacgat tggagatccc gcctcctttc agaccccgcc cgtgtggccg
     2821 cagcggcgag aactttgaca agttcttcac gcgggcggcg ccagcgctga cccctccaga
     2881 ccgcctagtc ctggccagca tcgaccaggc cgatttccag ggcttcacct acgtgaaccc
     2941 cgacttcgtg cacccggatg cccgcagccc caccagccca gtgcctgtgc ccgtcatgct
     3001 cgagtaccca tacgacgtac ctgattatgc aacttatcct tatgatgtac ctgattatgc
     3061 tagctatcct tatgatgtcc cagactacgc atagtctaga gggcccgttt aaacccgctg
     3121 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3181 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3241 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3301 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3361 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3421 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3481 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3541 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3601 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3661 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3721 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3781 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     3841 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3901 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3961 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4021 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4081 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4141 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4201 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgaccgagta caagcccacg
     4261 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     4321 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     4381 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4441 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4501 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4561 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4621 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4681 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4741 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4801 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag cgggactctg gggttcgaaa
     4861 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     4921 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     4981 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5041 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5101 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5161 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5221 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5281 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5341 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5401 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5461 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6001 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6121 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6181 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6241 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6301 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6361 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6421 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6481 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6541 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6601 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6661 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6721 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6781 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6841 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6901 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6961 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7021 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7081 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7141 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7201 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7261 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7321 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
//
