LOCUS       V028271                 4723 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028271
VERSION     V028271
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             841..870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             898..1836
                     /label="Prkra(NM_011871)"
                     /note="Prkra(NM_011871)"
                     /gene="Prkra"
     polyA_signal    1862..1996
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2107..2123)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2131..2147
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2155..2185)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2200..2221
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2509..3097)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3268..4128)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4129..4233)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4707..4723
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 gagcagaagc tgatctcaga ggaggacctg cttatggcca tggaggcccg aattcggatg
      901 tcccatagca ggcatcgtgc cgaggccccg ccgctgcagc gcgaggacag cgggaccttc
      961 agtttgggca agatgataac agctaagcct gggaaaacac cgattcaggt attgcacgag
     1021 tacggcatga agaccaagaa cattccagtt tatgaatgtg aaagatccga tgtgcaagta
     1081 cacgtgccca ctttcacctt cagagtaacc gttggtgaca taacttgcac aggtgaaggt
     1141 acaagtaaga agctggcgaa gcacagagct gcggaggccg ccataaacat tttgaaagcc
     1201 aatgcaagta tttgctttgc agttcctgac cccttaatgc ctgatccatc caaacagccg
     1261 aagaaccagc tgaatccaat tggctcatta caggaattag caattcacca tggctggcga
     1321 cttcctgaat ataccctttc ccaggaagga ggaccggctc ataagaggga atacaccacg
     1381 atctgcaggc ttgaatcatt catggaaact ggaaaggggg catcaaaaaa acaagccaag
     1441 agaaatgctg ctgagaaatt tctcgccaag tttagtaata tttctccaga gaaccacatt
     1501 tctctaacga acgtggttgg acattcccta ggatgcactt ggcactcctt gaggaattcc
     1561 cctggtgaga agatcaacct cctgaaaagg agcctcctca gtctcccgaa cacagactac
     1621 atccagctgc ttagtgagat tgccagcgag caaggcttta acataacgta tttggatata
     1681 gaggagctga gtgccaacgg acagtatcag tgtctcgcag agctgtccac cagtcctatc
     1741 accgtgtgtc acggctcagg catctcctgt ggcaatgcac agagtgacgc tgctcacaat
     1801 gctctgcagt atttaaagat aatagcagaa agaaagtagc tcgaggtacc gcggccgcgg
     1861 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1921 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1981 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     2041 ggtgtgggag gttttttcgg atcctctaga gtcgatctgc aggcatgcta gcttggcgta
     2101 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2161 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2221 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2281 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2341 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2401 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2461 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2521 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2581 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2641 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2701 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2761 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2821 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2881 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2941 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3001 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3061 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3121 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3181 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3241 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3301 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3361 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3421 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3481 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3541 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3601 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3661 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3721 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3781 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3841 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3901 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3961 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4021 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4081 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4141 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4201 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4261 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4321 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4441 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4501 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     4561 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4621 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4681 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agt
//
