LOCUS V039315 7317 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039315 VERSION V039315 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 71..3112 /label="PARP1(NM_001618)" /note="PARP1(NM_001618)" /gene="PARP1" CDS 3149..3613 /codon_start=1 /product="improved N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA" /label="VN155(I152L)" polyA_signal 3628..3762 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3873..3889) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3897..3913 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3921..3951) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3966..3987 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4275..4863) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5034..5894) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5895..5999) /gene="bla" /label="AmpR promoter" primer_bind 6473..6489 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 6516..6819 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 6820..7023 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 7161..7257 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" ORIGIN 1 atggcatcaa tgcagaagct gatctcagag gaggacctgc ttatggccat ggaggcccga 61 attcgccacc atggcggagt cttcggataa gctctatcga gtcgagtacg ccaagagcgg 121 gcgcgcctct tgcaagaaat gcagcgagag catccccaag gactcgctcc ggatggccat 181 catggtgcag tcgcccatgt ttgatggaaa agtcccacac tggtaccact tctcctgctt 241 ctggaaggtg ggccactcca tccggcaccc tgacgttgag gtggatgggt tctctgagct 301 tcggtgggat gaccagcaga aagtcaagaa gacagcggaa gctggaggag tgacaggcaa 361 aggccaggat ggaattggta gcaaggcaga gaagactctg ggtgactttg cagcagagta 421 tgccaagtcc aacagaagta cgtgcaaggg gtgtatggag aagatagaaa agggccaggt 481 gcgcctgtcc aagaagatgg tggacccgga gaagccacag ctaggcatga ttgaccgctg 541 gtaccatcca ggctgctttg tcaagaacag ggaggagctg ggtttccggc ccgagtacag 601 tgcgagtcag ctcaagggct tcagcctcct tgctacagag gataaagaag ccctgaagaa 661 gcagctccca ggagtcaaga gtgaaggaaa gagaaaaggc gatgaggtgg atggagtgga 721 tgaagtggcg aagaagaaat ctaaaaaaga aaaagacaag gatagtaagc ttgaaaaagc 781 cctaaaggct cagaacgacc tgatctggaa catcaaggac gagctaaaga aagtgtgttc 841 aactaatgac ctgaaggagc tactcatctt caacaagcag caagtgcctt ctggggagtc 901 ggcgatcttg gaccgagtag ctgatggcat ggtgttcggt gccctccttc cctgcgagga 961 atgctcgggt cagctggtct tcaagagcga tgcctattac tgcactgggg acgtcactgc 1021 ctggaccaag tgtatggtca agacacagac acccaaccgg aaggagtggg taaccccaaa 1081 ggaattccga gaaatctctt acctcaagaa attgaaggtt aaaaaacagg accgtatatt 1141 ccccccagaa accagcgcct ccgtggcggc cacgcctccg ccctccacag cctcggctcc 1201 tgctgctgtg aactcctctg cttcagcaga taagccatta tccaacatga agatcctgac 1261 tctcgggaag ctgtcccgga acaaggatga agtgaaggcc atgattgaga aactcggggg 1321 gaagttgacg gggacggcca acaaggcttc cctgtgcatc agcaccaaaa aggaggtgga 1381 aaagatgaat aagaagatgg aggaagtaaa ggaagccaac atccgagttg tgtctgagga 1441 cttcctccag gacgtctccg cctccaccaa gagccttcag gagttgttct tagcgcacat 1501 cttgtcccct tggggggcag aggtgaaggc agagcctgtt gaagttgtgg ccccaagagg 1561 gaagtcaggg gctgcgctct ccaaaaaaag caagggccag gtcaaggagg aaggtatcaa 1621 caaatctgaa aagagaatga aattaactct taaaggagga gcagctgtgg atcctgattc 1681 tggactggaa cactctgcgc atgtcctgga gaaaggtggg aaggtcttca gtgccaccct 1741 tggcctggtg gacatcgtta aaggaaccaa ctcctactac aagctgcagc ttctggagga 1801 cgacaaggaa aacaggtatt ggatattcag gtcctggggc cgtgtgggta cggtgatcgg 1861 tagcaacaaa ctggaacaga tgccgtccaa ggaggatgcc attgagcact tcatgaaatt 1921 atatgaagaa aaaaccggga acgcttggca ctccaaaaat ttcacgaagt atcccaaaaa 1981 gttctacccc ctggagattg actatggcca ggatgaagag gcagtgaaga agctgacagt 2041 aaatcctggc accaagtcca agctccccaa gccagttcag gacctcatca agatgatctt 2101 tgatgtggaa agtatgaaga aagccatggt ggagtatgag atcgaccttc agaagatgcc 2161 cttggggaag ctgagcaaaa ggcagatcca ggccgcatac tccatcctca gtgaggtcca 2221 gcaggcggtg tctcagggca gcagcgactc tcagatcctg gatctctcaa atcgctttta 2281 caccctgatc ccccacgact ttgggatgaa gaagcctccg ctcctgaaca atgcagacag 2341 tgtgcaggcc aaggtggaaa tgcttgacaa cctgctggac atcgaggtgg cctacagtct 2401 gctcagggga gggtctgatg atagcagcaa ggatcccatc gatgtcaact atgagaagct 2461 caaaactgac attaaggtgg ttgacagaga ttctgaagaa gccgagatca tcaggaagta 2521 tgttaagaac actcatgcaa ccacacacaa tgcgtatgac ttggaagtca tcgatatctt 2581 taagatagag cgtgaaggcg aatgccagcg ttacaagccc tttaagcagc ttcataaccg 2641 aagattgctg tggcacgggt ccaggaccac caactttgct gggatcctgt cccagggtct 2701 tcggatagcc ccgcctgaag cgcccgtgac aggctacatg tttggtaaag ggatctattt 2761 cgctgacatg gtctccaaga gtgccaacta ctgccatacg tctcagggag acccaatagg 2821 cttaatcctg ttgggagaag ttgcccttgg aaacatgtat gaactgaagc acgcttcaca 2881 tatcagcaag ttacccaagg gcaagcacag tgtcaaaggt ttgggcaaaa ctacccctga 2941 tccttcagct aacattagtc tggatggtgt agacgttcct cttgggaccg ggatttcatc 3001 tggtgtgaat gacacctctc tactatataa cgagtacatt gtctatgata ttgctcaggt 3061 aaatctgaag tatctgctga aactgaaatt caattttaag acctccctgt ggggtaccgg 3121 aggtggcggg agcggaggtg gcgggagtat ggtgagcaag ggcgaggagc tgttcaccgg 3181 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 3241 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac 3301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg 3361 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 3421 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 3481 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 3541 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 3601 ctatctcacc gcctgagcgg ccgcggggat ccagacatga taagatacat tgatgagttt 3661 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 3721 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 3781 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcggatcc tctagagtcg 3841 atctgcaggc atgctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 3901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 3961 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 4021 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 4081 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 4501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 4861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 4921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 4981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 5461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 5521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 5941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6001 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 6061 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 6121 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 6181 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 6241 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 6301 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt 6361 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 6421 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 6481 acggccagtg agttcgagct tgcatgcctg caggtcgtta cataacttac ggtaaatggc 6541 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc 6601 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact 6661 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat 6721 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact 6781 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac 6841 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac 6901 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac 6961 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga 7021 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat 7081 agaagacacc gggaccgatc cagcctccgg actctagagg atccggtact agaggaactg 7141 aaaaaccaga aagttaactg gtaagtttag tctttttgtc ttttatttca ggtcccggat 7201 ccggtggtgg tgcaaatcaa agaactgctc ctcagtggat gttgccttta cttctaggcc 7261 tgtacggaag tgttacttct gctctaaaag ctgcggaatt gtacccgcgg gcccacc //