LOCUS       V021659                 6194 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021659
VERSION     V021659
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1357..2811
                     /label="ZDHHC1(NM_013304)"
                     /note="ZDHHC1(NM_013304)"
                     /gene="ZDHHC1"
     polyA_signal    2982..3103
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3110..3565)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3592..3696
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3698..4055
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4090..4884
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5116..5163
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5492..6080
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagctatgt acaagatgaa catctgcaac
     1381 aagccctcca acaagacggc ccctgagaag agtgtgtgga cggcaccggc acagcccagc
     1441 ggaccctccc ctgagctgca gggccagcga tcccgccgga atgggtggag ctggccccct
     1501 cacccgctcc agattgtggc ctggctgctg tacctcttct ttgctgtgat cggctttggg
     1561 atccttgttc ccctcctgcc tcaccactgg gtgcccgctg gctacgcttg catgggcgcc
     1621 atctttgctg gccaccttgt ggtgcacctg accgccgtct ccatcgatcc agcagatgcc
     1681 aacgtgcggg acaagagcta tgcggggccc ctgcccatct tcaaccgaag ccagcacgca
     1741 catgtcattg aagacctgca ctgcaacttg tgcaacgtgg atgtgagcgc tcgctccaag
     1801 cactgcagcg cctgcaacaa gtgcgtgtgc ggtttcgacc accactgcaa gtggctcaac
     1861 aactgtgtgg gcgagcggaa ctaccggctc tttctacaca gtgttgcatc cgctttactg
     1921 ggcgtcctgc tcctggtgct ggtggccaca tatgtcttcg tggagttctt tgtcaacccc
     1981 atgcgtctgc gcaccaaccg acactttgaa gtcctgaaga atcacacgga tgtgtggttc
     2041 gtgttcctgc ctgccgcccc cgtggagacc caggcccctg ccatcctggc cctggccgcc
     2101 ctgctcatcc ttctgggcct cctgtccaca gccctcctgg ggcacctgct ctgcttccac
     2161 atttatctca tgtggcacaa gctcaccacc tatgagtaca tcgtgcagca ccgcccacca
     2221 caggaggcca agggggttca cagggagctc gagtcatgtc ctcccaagat gcggcccatt
     2281 caggagatgg agttctacat gcggaccttc agacatatgc gcccagagcc ccctggccag
     2341 gccgggccag cagcagtgaa tgccaaacac tctcgccctg cctccccgga tccgacccca
     2401 ggtaggaggg actgtgctgg gcctccggtc caggtggagt gggatagaaa gaagcctcta
     2461 ccctggcgct cgcctctgct tcttttggcg atgtggggcc ctcaggctcc cccgtgtctc
     2521 tgcagaaaaa gaggaagagg cgcgtgtata aagtgcgaac gtctgagacc tcggatccgg
     2581 cgtcggggcc tagggccccc agccgccgct ccagctcgtc gacggattcc gcggacgcca
     2641 gccctgtgca cgccgctggc cctgccggcg cctaccactc ggcgtcggca gagtccgtgg
     2701 acgagattcc agtggcgcag acgcgcctgg gcagcgccgc tctggccgcc ccgcggggcc
     2761 ggggccgaca gcccacgctg gcgcggcagg cgcgtgcgcc cgccgttttc gtgaaagctt
     2821 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
     2881 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2941 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     3001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc
     3121 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3181 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3241 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3301 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3361 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3421 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3481 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3541 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3601 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3661 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     3721 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3781 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     3841 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     3901 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3961 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4021 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     4081 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4141 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4201 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4261 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4321 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4381 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4441 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     4501 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     4561 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     4621 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     4681 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     4741 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     4801 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     4861 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     4921 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     4981 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5041 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     5101 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     5161 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     5221 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     5281 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     5341 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5401 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5461 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5521 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5581 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5641 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5701 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5761 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5821 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5881 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5941 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6001 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6061 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6121 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6181 ttaccgccat gcat
//
