LOCUS       V026897                 6479 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026897
VERSION     V026897
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             603..1301
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric blue fluorescent protein"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
                     /label="TagBFP"
     CDS             1353..3029
                     /label="YTHDF1(NM_017798)"
                     /note="YTHDF1(NM_017798)"
                     /gene="YTHDF1"
     CDS             3036..3101
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3267..3388
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3395..3850)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3877..3981
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3983..4340
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4375..5169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5401..5448
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5777..6365
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
      661 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
      721 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
      781 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
      841 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      901 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
      961 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     1021 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     1081 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
     1141 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     1201 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     1261 gatactgcga cctccctagc aaactggggc acaagcttaa tggcggcgga gggagtggag
     1321 gagggggaag cggaggcgga gggagtctcg agatgtcggc caccagcgtg gacacccaga
     1381 gaacaaaagg acaagataat aaagtacaaa atggttcgtt acatcagaag gatacagttc
     1441 atgacaatga ctttgagccc taccttactg gacagtcaaa tcagagtaac agttacccct
     1501 caatgagcga cccctacctg tccagctatt acccgccgtc cattggattt ccttactccc
     1561 tcaatgaggc tccgtggtct actgcagggg accctccgat tccatacctc accacctacg
     1621 gacagctcag taacggagac catcatttta tgcacgatgc tgtttttggg cagcctgggg
     1681 gcctggggaa caacatctat cagcacaggt tcaatttttt ccctgaaaac cctgcgttct
     1741 cagcatgggg gacaagtggg tctcaaggtc agcagaccca gagctccgcg tatgggagca
     1801 gctacaccta ccccccgagc tccctgggtg gcacggtggt tgatgggcag ccaggctttc
     1861 acagcgacac cctcagcaag gcccccggga tgaacagcct ggagcagggc atggttggcc
     1921 tgaagattgg ggacgtcagc tcctccgccg tcaagacggt gggctctgtc gtcagcagcg
     1981 tggcactgac tggtgtcctt tctggcaacg gtgggacaaa tgtgaacatg ccagtttcaa
     2041 agccgacctc gtgggctgcc attgccagca agcctgcaaa accacagcct aaaatgaaaa
     2101 caaagagcgg gcctgtcatg gggggtgggc tgccccctcc acccataaag cataacatgg
     2161 acattggcac ctgggataac aaggggcctg tgccgaaggc cccagtcccc cagcaggcac
     2221 cctctccaca ggctgcccca cagccccagc aggtggctca gcctctccca gcacagcccc
     2281 cagctttggc tcaaccgcag tatcagagcc ctcagcagcc accccagacc cgctgggttg
     2341 ccccacgcaa cagaaacgcg gcgtttgggc agagcggagg ggctggcagc gatagcaact
     2401 ctcctggaaa cgtccagcct aattctgccc ccagcgtcga atcccacccc gtccttgaaa
     2461 aactgaaggc tgctcacagc tacaacccga aagagtttga gtggaatctg aaaagcgggc
     2521 gtgtgttcat catcaagagc tactctgagg acgacatcca ccgctccatt aagtactcca
     2581 tctggtgtag cacagagcac ggcaacaagc gcctggacag cgccttccgc tgcatgagca
     2641 gcaaggggcc cgtctacctg ctcttcagcg tcaatgggag tgggcatttt tgtggggtgg
     2701 ccgagatgaa gtcccccgtg gactacggca ccagtgccgg ggtctggtct caggacaagt
     2761 ggaaggggaa gtttgatgtc cagtggattt ttgttaagga tgtacccaat aaccagctcc
     2821 ggcacatcag gctggagaat aacgacaaca aaccggtcac aaactcccgg gacacccagg
     2881 aggtgccctt agaaaaagcc aagcaagtgc tgaaaattat cagttcctac aagcacacaa
     2941 cctccatctt cgacgacttt gctcactacg agaagcgcca ggaggaggag gaggtggtgc
     3001 gcaaggaacg gcagagtcga aacaaacaag agctcgacta caaagaccat gacggtgatt
     3061 ataaagatca tgacatcgac tacaaggatg acgatgacaa gtgaattctg cagtcgacgg
     3121 taccgcgggc ccgggatcca ccggtgcggc cgcgactcta gatcataatc agccatacca
     3181 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3241 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3301 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3361 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     3421 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3481 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3541 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3601 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3661 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3721 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3781 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3841 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3961 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4021 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4081 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4141 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4201 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4261 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4321 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4381 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4441 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4501 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4561 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4621 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4681 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4741 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4801 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4861 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4921 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4981 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5041 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5101 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5161 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5221 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5281 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5341 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     5401 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5461 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5521 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5581 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     5641 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5701 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5761 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5821 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5881 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5941 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6001 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6061 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6421 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
